Synthesis and characterization of a paramagnetic sialic acid conjugate as probe for magnetic resonance applications

11 páginas, 8 figuras, 4 tablas, 3 esquemas -- PAGS nros. 21-31Magnetic Resonance Imaging (MRI) using paramagnetic systems as contrast agents is receiving increased attention as diagnostic tool in the clinic. At the same time, NMR of paramagnetic systems can also be applied in biochemical fields; for example, the use of Paramagnetic Relaxation Enhancement (PRE) allows structure refinement and the analysis of transient dynamic processes involved in macromolecular complex formation. Herein we report the synthesis and computational characterization of a new DOTA-like sialic acid conjugate, which can be used both in MRI and PRE applications when coordinated to a suitable paramagnetic metalWe gratefully acknowledge financial supports from the University of Milano-Bicocca (FAR2008) and computational facilities from CILEA (Consorzio Interuniversitario Lomabardo per l’Elaborazione Automatica), Programma Operativo Regione Lombardia Ob. 3 Fondo Sociale Europeo 2000–2006, Sovvenzione Globale Progetto Ingenio A0001127/2007 and Consorzio Interuniversitario Nazionale ‘Metodologie e Processi Innovativi di Sintesi’ (C.I.N.M.P.I.S.).Peer reviewe

Synthesis and characterization of a paramagnetic sialic acid conjugate as probe for magnetic resonance applications

11 páginas, 8 figuras, 4 tablas, 3 esquemas -- PAGS nros. 21-31Magnetic Resonance Imaging (MRI) using paramagnetic systems as contrast agents is receiving increased attention as diagnostic tool in the clinic. At the same time, NMR of paramagnetic systems can also be applied in biochemical fields; for example, the use of Paramagnetic Relaxation Enhancement (PRE) allows structure refinement and the analysis of transient dynamic processes involved in macromolecular complex formation. Herein we report the synthesis and computational characterization of a new DOTA-like sialic acid conjugate, which can be used both in MRI and PRE applications when coordinated to a suitable paramagnetic metalWe gratefully acknowledge financial supports from the University of Milano-Bicocca (FAR2008) and computational facilities from CILEA (Consorzio Interuniversitario Lomabardo per l’Elaborazione Automatica), Programma Operativo Regione Lombardia Ob. 3 Fondo Sociale Europeo 2000–2006, Sovvenzione Globale Progetto Ingenio A0001127/2007 and Consorzio Interuniversitario Nazionale ‘Metodologie e Processi Innovativi di Sintesi’ (C.I.N.M.P.I.S.).Peer reviewe