2 research outputs found
Influence of breastfeeding versus formula feeding on lymphocyte subsets in infants at risk of coeliac disease: the PROFICEL study.
10 páginas.Purpose: In addition to genetic risk, environmental factors might influence celiac disease (CD)
development. We sought to assess the effect of the interaction between milk-feeding practices and the
HLA-DQ genotype, on peripheral lymphocyte subsets and their activation markers in infants at familial
risk for CD.
Methods: 170 newborns were classified in 3 different genetic risk groups (high risk, HR; intermediate
risk, IR; and low risk, LR) after DQB1 and DQA1 typing. Lymphocyte subsets were studied at the age of
4 months by flow cytometry analysis.
Results: 79 infants were receiving exclusive breast-feeding (BF), and 91 partial breast-feeding or
formula-feeding (FF). Regarding genetic risk, 40 infants were classified in HR group, 75 in IR group and
55 in LR group. Two-way ANOVA did not show significant interactions between the type of milkfeeding
and genetic risk group on the lymphocyte subsets analysed. One-way ANOVA for milk-feeding
practice alone showed that the percentage of CD4+CD25+ cells was significantly higher in BF group than
in FF group (BF, 10.92±2.71; FF, 9.94±2.96; p=0.026), and absolute counts of CD4+CD38+ cells were
significantly higher in FF group than in BF group (FF, 2881.23±973.48; BF, 2557.95±977.06; p=0.038).
One-way ANOVA for genetic risk alone showed that absolute counts of NK cells were significantly
higher in IR group than HR and LR groups (IR, 539.24±340.63; HR, 405.01±239.53; LR, 419.86±262.85;
p=0.028).
Conclusion: Lymphocyte subset profiles in the early stages of life could be modulated by milk-feeding
practices and genetic risk separately. Breast-feeding might have a positive immunomodulatory effect on
lymphocyte subsets in infants at risk for CD.supported by grants AGL2007-66126-C03-01/ALI, AGL2007-66126-C03-02/ALI and
AGL2007-66126-C03-03/ALI, from the Spanish Ministry of Science and Innovation and grants
200570F0091 and 200570F0093 from CSIC.Peer reviewe