1 research outputs found
Spectroscopic study of the interaction of actinomycin D with oligonucleotides carrying the central base sequences -XGCY- and -XGGCCY- using multivariate methods
10 pages, 5 figures, 1 table.-- PMID: 17123067 [PubMed].-- Published online Nov 23, 2006.Supporting information (4 pages, 3 suppl. figures) available at: http://www.springerlink.com/content/870650451902431q/MediaObjects/216_2006_946_MOESM1_ESM.docThe interactions of actinomycin D (ACTD) with
the oligonucleotides 5′-CAAAGCTTTG-3′, 5′-CATGGC
CATG-3′ and 5′-TATGGCCATA-3′ were investigated by
means of acid–base titrations and mole-ratio and melting experiments monitored by molecular absorption and circular dichroism (CD) spectroscopies. For each experiment, CD and molecular absorption spectra were recorded at each point in the experiment, and later analyzed via appropriate multivariate data analysis methods. The study of the interactions between these oligonucleotides and ACTD at 25°C showed the formation of an interaction complex with
a stoichiometry of 1:1 (ACTD:duplex) and values for the log(formation constant) of 5.1 ± 0.3, 6.4 ± 0.2, and 5.6 ± 0.2, respectively. An additional interaction complex at higher
temperatures was also detected, which might be related to the single-stranded forms of the oligonucleotides.We acknowledge two grants from the Spanish
Ministerio de Educación y Ciencia (projects BFU2004-02048/BMC and BQU2003-0191).Peer reviewe