2 research outputs found
Messenger RNAs bearing tRNA-like features exemplified by interferon alfa 5 mRNA
Abstract The purpose of this work was to ascertain
whether liver mRNA species share common structural
features with hepatitis C virus (HCV) mRNA that allow
them to support the RNase-P (pre-tRNA/processing enzyme)
cleavage reaction in vitro. The presence of RNase-P
competitive elements in the liver mRNA population was
determined by means of biochemical techniques, and a set
of sensitive mRNA species were identified through microarray
screening. Cleavage specificity and substrate
length requirement of around 200 nts, were determined for
three mRNA species. One of these cleavage sites was
found in interferon-alpha 5 (IFNA5) mRNA between
specific base positions and with the characteristic RNase-P
chemistry of cleavage. It was mapped within a cloverleaflike
structure revealed by a comparative structural analysis
based on several direct enzymes and chemical probing
methods of three RNA fragments of increasing size, and
subsequently contrasted against site-directed mutants. The
core region was coincident with the reported signal for the
cytoplasmic accumulation region (CAR) in IFNAs.This research was funded by the Ministerio de Ciencia e Innovaci贸n
BIO2010-1521 and BFU2012-35898. Proyecto Excelencia Junta de
Andaluc铆a CVI-03050 and CIBERehd.Peer reviewe