2 research outputs found
Comparative methods for the evaluation of protein adsorption
AFM, Immunogold labeling and SPR have been successfully applied to the analysis of protein
adsorption on fibroin substrates, chosen as a paradigmatic biomaterial. The process was
evaluated both under static and dynamic conditions, also considering the effect of protein
competition. AFM via antibody-modified tips provided maps of protein adsorption and
information on protein-protein interactions. Double labeling Immunogold imaged protein
distribution upon competitive adsorption. SPR
described the dynamics of the process, offering
also quantitative data both from individual and
two-component protein solutions. The integration
of these analytical techniques and the
critical evaluation of the results can provide a
detailed description of protein adsorption.Peer reviewe