2 research outputs found
In situ and postâsynthesis immobilization of enzymes on nanocrystalline MOF platforms to yield active biocatalysts
[Background] Very recently, metalâorganic framework (MOF) materials have been postulated as emerging supports to achieve solidâstate enzymeâcontained biocatalysts. In this work, postâsynthesis and in situ strategies to immobilize ÎČâglucosidase and laccase on different MOF materials were studied. The MOFâbased supports, i.e. MILâ53(Al), NH2âMILâ53(Al) and MgâMOFâ74, were prepared under soft and sustainable conditions (room temperature and pH values compatible with enzymatic activity), allowing development of the in situ strategy.[Results] In both postâsynthesis and in situ approaches, the intercrystalline mesoporosity of the MOFâbased support favored the immobilization efficiency or the specific activity. The latter expressed as units per milligram of immobilized enzyme was higher in the postâsynthesis immobilization, whereas the biocatalysts prepared in situ gave much higher enzyme loading (over 85%) and lower enzyme leaching (around 5%). The in situ approach even worked in a nonâaqueous (N,Nâdimethylformamide) media in which the free enzyme was completely inactive. The immobilized enzymes are much larger than the structural pores of the MOFs.[Conclusions] Enzymes can be efficiently immobilized on nanocrystalline MOFs prepared under soft and sustainable conditions despite the supports lacking large enough pores to host the enzymes. The in situ approach is very efficient capturing enzymes and preserving some of their activity even under adverse conditions. © 2017 Society of Chemical IndustryThis work has been partially financed by the Spanish State Research Agency (Agencia Española de InvestigaciĂłn, AEI) and the European Regional Development Fund (Fondo Europeo de Desarrollo Regional, FEDER) through the Project MAT2016â77496âR (AEI/FEDER, UE).Peer reviewe