2 research outputs found
Capillary electrophoresis-mass spectrometry of Spirulina platensis proteins obtained by pressurized liquid extraction
In this work, the usefulness of CE-MS to monitor and optimize the pressurized liquid
extraction (PLE) of proteins from Spirulina platensis microalga is demonstrated. Crude
and purified PLE extracts from microalga were analyzed by CE-MS. It was observed
that the use of purification protocols of phycobiliproteins (namely, ultrafiltration or precipitation-
dialysis-freeze drying) resulted in better CE resolution and MS signals,
demonstrating that sample matrix plays an important role in CE-MS of proteins in real
samples. Ultrafiltration was found less laborious and much faster than precipitationdialysis-
freeze drying (1 vs. 48 h). Direct analysis of crude extracts was demonstrated
to be also possible by CE-MS, providing less-quality information but enough to characterize
PLE extracts in a much faster way. Therefore, the latter protocol was selected
to monitor and optimize the extraction process of phycobiliproteins from S. platensis.
To do that, different extraction conditions were tested, including time, temperature and
pressure of extraction, nature of pressurized liquid, distribution of microalga inside the
extraction cell, type of packing, etc. It is demonstrated that the combined use of PLE
and CE-MS allows the attainment of extracts rich in phycobiliproteins in short extraction
times (namely, yields of 20% can be obtained in less than 2 h under the optimum
PLE process in an automatic way). To our knowledge, this work shows for the first time
the usefulness of CE-MS for monitoring and optimizing a PLE process.Financial support from AGL2002–04621-C02–02 project
(CICYT, Ministerio de Ciencia y TecnlogÃa) is acknowledged.
M.H. thanks MCYT for an FPI grant. C.S. thanks
ConsejerÃa de Educación y Cultura (Comunidad de
Madrid) for a fellowship.Peer reviewe