Looking into the Evolution of Mandarin duck Group from Red Rose

《红玫瑰》创刊于1924年7月2日，终刊于1932年1月28日，共出版350期，出版历时长达七年之久，是现代通俗文学期刊中寿命最长的刊物之一，被公认为鸳鸯蝴蝶派的代表刊物，展现了通俗文学杂志的第二代风貌。作为一份大众通俗刊物，《红玫瑰》在二十世纪二三十年代的上海非常流行，深受市民读者喜爱，但在接下来的时间长期遭受人们的非议与批判，并未得到国内学术界应有的客观且公正的评价。八十年代中期后，随着学术界对鸳鸯蝴蝶派及通俗文学研究的深入，这份期刊成为鸳鸯蝴蝶派研究的原始佐证史料不断被提及，但遗憾的是将其作为研究对象的专文专著尚未出现。笔者力图通过翔实的史料研究梳理与突入文本内部的感性体认，还《红玫瑰》..."Red Rose," was published in the July 2, 1924, finally published in the January 28, 1932, a total of 350 publications, lasting for seven years. It is the longest publication in modern popular literature periodicals, and it is recognized as the behalf publications of "Mandarin duck Group", for it shows the "the second-generation style of Popular Literature magazine". As a popular literary publicati...学位：文学硕士院系专业：人文学院中文系_中国现当代文学学号：2005130005

Satellite observation of the temporal and spatial variation of sea surface diurnal warming in the South China Sea

利用搭载在AQuA和TErrA卫星上的MOdIS(MOdErATE rESOluTIOn IMAgIng SPECTrOrAdIOMETEr)、AMSr-E(AdVAnCEd MICrOWAVE SCAnnIng rAdIOMETEr fOr THE EArTH ObSErVIng SySTEM)传感器测量反演的昼夜海表温度(SST),计算海表面日增温(SEA SurfACE dIurnAl WArMIng),分析南海海表面日增温的短期和年变动特征。受观测平台过境时间、传感器测量SST方式、反演算法等影响,MOdIS/AQuA计算的日增温幅度略大于AMSr-E/AQuA和MOdIS/TErrA,但在表征南海海表面日增温的时空分布特征以及变化趋势上三者并未见显著性差异。南海海表面日增温在时间分布上以冬季为最小,春季为最大;在空间分布上则是南部海域大于中部和北部海域,东部海域大于西部海域。春夏之交的吕宋海峡西北部尤其容易发生日增温事件。海表面日增温与太阳辐射、风速、云量等影响有关,其中风速与海表面日增温显著负相关。Satellite-derived sea surface temperature(SST) data from the MODIS(moderate resolution imaging spectroradiometer)/ Aqua, AMSR-E(advanced microwave scanning radiometer for the earth observing system)/Aqua and MODIS/Terra over the past ~10 years are analyzed to investigate the sea surface diurnal warming in the South China Sea(SCS).The results reveal that sea surface diurnal warming derived by MODIS/Aqua is slightly higher than that by AMSR-E/Aqua and MODIS/Terra, due to the differences in satellite transit time, sampling manner and retrieval algorithm, among others.However, there are no significant biases in terms of spatial and temporal distributions, and of the variation of sea surface diurnal warming over the SCS.The magnitude of sea surface diurnal warming in the SCS is the weakest during winter, and the strongest during spring.The magnitude is generally larger in the southern regions than in the central and northern regions, and is larger in the eastern regions than in the western regions.Northwest of the Luzon Strait, the sea surface diurnal warming tends to appear easily from late spring to early summer.It is suggested that the seasonal variation of sea surface diurnal warming in the SCS be affected by solar radiation, wind speed and cloud amount; among them, the wind speed is the most important factor with a significant negative correlation with the diurnal warming.福建省自然科学基金项目(2011J01278); 国家自然科学基金项目(40706041

Cloning and characterization of G protein beta 1 subunit in shrimp Litopenaeus vannamei

根据G蛋白Gβ1亚基的保守序列设计简并引物,通过简并PCR和RACE技术,克隆到凡纳滨对虾(Litopenaeusvannamei)Gβ1基因的全长cDNA序列。利用Blast、DNAstar和Genedoc软件分析,发现该Gβ1编码的蛋白序列与其他物种已知的Gβ1序列具有相当高的保守性,将它命名为pvGβ1。免疫共沉淀分析发现pvGβ1能在体外适当条件下与对虾Gαs或Gαq相互结合。Westernblot-ting分析发现,pvGβ1在对虾身体各部位都有广泛分布,尤其在脑神经、眼和眼柄有大量表达。说明了Gβ1在对虾的神经系统和光信号传导等生命过程中的重要性。 【英文摘要】 The β/γ-subunits of the heterotrimeric GTP-binding proteins are important regulators of G-protein alpha subunits as well as a series of signal transduction receptors and effectors. In this study, a novel G protein β_1 subunit was isolated from shrimp Litopenaeus vannamei, and was termed pvGβ_1. Sequence analysis showed that the pvGβ_1 protein contained all the well-conserved domains and motifs that were critical sites for interaction with receptors and other binding effectors. Co-immunoprecipitation assay d...国家863计划项目(2002AA629060)资

红树林土壤中脂肪酶产生菌的筛选及酶学性质

从福建龙海红树林区土壤中分离获得9株产脂肪酶的细菌,经16S rDNA序列分析表明分别属于红球菌属(Rhodococcus)、库克菌属(Kocuria)、假单胞菌属(Pseudomonas)、芽胞杆菌属(Bacillus)和微小杆菌属(Exiguobacteri-um).对9株细菌所产的脂肪酶进行酶学性质研究,结果显示这些酶的最适作用温度在25~35℃、最适作用pH值在8~10,其中L13菌株所产脂肪酶(L′13)具有适应温度和pH范围较广、对多种金属离子耐受性强、能有效降解不同底物等特点,在环境保护和工业生产方面具有良好的应用前景

Determinants that control the specific interactions between TAB1 and p38α

Previous studies have revealed that transforming growth factor-beta-activated protein kinase 1 (TAB1) interacts with p38 alpha and induces p38 alpha autophosphorylation. Here, we examine the sequence requirements in TAB1 and p38 alpha that drive their interaction. Deletion and point mutations in TAB1 reveal that a proline residue in the C terminus of TAB1 (Pro412) is necessary for its interaction with p38 alpha. Furthermore, a cryptic D-domain-like docking site was identified adjacent to the N terminus of Pro412, putting Pro412 in the (phi(B)+3 position of the docking site. Through mutational analysis, we found that the previously identified hydrophobic docking groove in p38 alpha is involved in this interaction, whereas the CD domain and ED domain are not. Furthermore, chimeric analysis with p38 beta (which does not bind to TAB1) revealed a previously unidentified locus of p38 alpha comprising Thr218 and Ile275 that is essential for specific binding of p38 alpha to TAB1. Converting either of these residues to the corresponding amino acid of p380 abolishes p38 alpha interaction with TAB1. These p38a mutants still can be fully activated by p38 alpha upstream activating kinase mitogen-activated protein kinase kinase 6, but their basal activity and activation in response to some extracellular stimuli are reduced. Adjacent to Thr218 and Ile275 is a site where large conformational changes occur in the presence of docking-site peptides derived from p38 alpha substrates and activators. This suggests that TAB1-induced autophosphorylation of p38 alpha results from conformational changes that are similar but unique to those seen in p38 alpha interactions with its substrates and activating kinases

间充质干细胞在器官移植中发挥免疫抑制作用及机制探讨的研究进展

器官移植是终末期器官衰竭患者最有效的治疗手段。将间充质干细胞(MSC)用于器官移植已成为细胞疗法的重要组成部分。然而,MSC发挥免疫抑制作用的机制还有待进一步地挖掘,且影响MSC发挥免疫抑制作用的因素很多,这些原因导致MSC难以达到预期疗效。在本综述中将通过介绍MSC的免疫抑制作用及机制、影响MSC发挥免疫抑制作用的因素以及MSC的临床应用等方面来阐述MSC在器官移植领域的研究进展。国家重点研发项目(2018YFA0108304)国家自然科学基金(81771721、81671583

Rat glioma C6 cell apoptosis induced by UV radiation via p38-MAPK

目的 :探讨 p38MAPK在紫外线损伤刺激细胞中的特异性信号转导作用 .方法 :流式细胞仪检测紫外线照射 30min后 1,2及 4h的C6细胞周期变化和是否有凋亡发生 ;应用免疫细胞化学技术观察紫外线刺激前后 p38MAPK在C6细胞中的表达强度和分布特征 .结果 :细胞周期结果显示 1,2和 4h后G1期细胞数分数各增多 0 .12 ,0 .2 1和 0 .19,而S期细胞数分数减少 0 .10 ,0 .14和 0 .15 ;各组的凋亡率分别是12 % ,4 9%和 34% ;未受刺激的细胞中 ,p38MAPK在胞质和胞核表达较弱 ;紫外线损伤作用 2h后 ,细胞核区的染色强度即明显增强 ,而胞质区域的染色强度相对降低 .结论 :C6细胞受紫外线损伤后可通过 p38MAPK通路发生凋亡. 【英文摘要】 AIM: To study the signal transduction of p38 mitogen activated protein kinase in rat glioma C6 cells after the stimulation of UV radiation. METHODS: Flow cytometry was applied to measure the fraction number changes in the cell cycle phase and to detect whether UV could induce apoptosis of C6 cells. The level and distribution of p38MAPK expression was examined by immunocytochemical method both before and after the UV radiation. RESULTS: Flow cytometry indicated that the numbers of G1 phase fraction of 1,...高等学校骨干教师计划资助 ;; 留学归国人员科研启动基金([1 999] 747号

AMP as a Low-Energy Charge Signal Autonomously Initiates Assembly of AXIN-AMPK-LKB1 Complex for AMPK Activation

The AMP-activated protein kinase (AMPK) is a master regulator of metabolic homeostasis by sensing cellular energy status. AMPK is mainly activated via phosphorylation by LKB1 when cellular AMP/ADP levels are increased. However, how AMP/ADP brings about AMPK phosphorylation remains unclear. Here, we show that it is AMP, but not ADP, that drives AXIN to directly tether LKB1 to phosphorylate AMPK. The complex formation of AXIN-AMPK-LKB1 is greatly enhanced in glucose-starved or AICAR-treated cells and in cell-free systems supplemented with exogenous AMP. Depletion of AXIN abrogated starvation-induced AMPK-LKB1 colocalization. Importantly, adenovirus-based knockdown of AXIN in the mouse liver impaired AMPK activation and caused exacerbated fatty liver after starvation, underscoring an essential role of AXIN in AMPK activation. These findings demonstrate an initiating role of AMP and demonstrate that AXIN directly transmits AMP binding of AMPK to its activation by LKB1, uncovering the mechanistic route for AMP to elicit AMPK activation by LKB1.http://news.xmu.edu.cn/s/13/t/542/22/a9/info139945.ht

The Lysosomal v-ATPase-Ragulator Complex Is a Common Activator for AMPK and mTORC1, Acting as a Switch between Catabolism and Anabolism

林圣彩教授课题组长期致力于细胞信号转导的研究。近年来，该课题组潜心研究，不断攻关，取得了一系列重大成果，如揭示细胞如何应对生长因子缺乏的内在机理，发现了细胞自噬“路线图”、还发现了细胞如何感应“饥饿”信号AMP的信号传导通路等。其中，“发现细胞自噬‘路线图’”成果曾登上《科学》杂志，并入选2012年度“中国科学十大进展”。AMPK and mTOR play principal roles in governing metabolic programs; however, mechanisms underlying the coordination of the two inversely regulated kinases remain unclear. In this study we found, most surprisingly, that the late endosomal/lysosomal protein complex v-ATPase-Ragulator, essential for activation of mTORC1, is also required for AMPK activation. We also uncovered that AMPK is a residential protein of late endosome/lysosome. Under glucose starvation, the v-ATPase-Ragulator complex is accessible to AXIN/LKB1 for AMPK activation. Concurrently, the guanine nucleotide exchange factor (GEF) activity of Ragulator toward RAG is inhibited by AXIN, causing dissociation from endosome and inactivation of mTORC1. We have thus revealed that the v-ATPase-Ragulator complex is also an initiating sensor for energy stress and meanwhile serves as an endosomal docking site for LKB1-mediated AMPK activation by forming the v-ATPase-Ragulator-AXIN/LKB1-AMPK complex, thereby providing a switch between catabolism and anabolism. Our current study also emphasizes a general role of late endosome/lysosome in controlling metabolic programs

单磷酸腺苷激活的蛋白激酶(AMPK)调控机制的研究进展

代谢是细胞和有机体最基本、最重要的活动之一.细胞和有机体通过感应系统实时监测代谢过程中的物质和能量状态,并不断地通过错综复杂的代谢调控途径来维持其稳态.代谢调控一旦出现紊乱,机体代谢就会发生异变,从而导致如糖尿病、肥胖、心血管疾病乃至肿瘤等多种人类重大疾病的发生,并影响发育、生长、繁殖、衰老等生命过程.单磷酸腺苷激活的蛋白激酶(AMPK)作为在代谢调控中起核心作用的激酶,自其被发现以来一直是研究的热点.对AMPK调控机制的深入研究为揭示代谢性疾病的发生和发展机制、探索其治疗和预防的策略提供了重要的新思路.围绕近年来国内外AMPK研究领域取得的进展,重点阐述AMPK在体内的激活机制以及本课题组在该领域中的一系列重要成果.国家自然科学基金(31370744