12 research outputs found

    The Combination of Three Natural Compounds Effectively Prevented Lung Carcinogenesis by Optimal Wound Healing

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    <div><p>The tumor stroma has been described as “normal wound healing gone awry”. We explored whether the restoration of a wound healing-like microenvironment may facilitate tumor healing. Firstly, we screened three natural compounds (shikonin, notoginsenoside R1 and aconitine) from wound healing agents and evaluated the efficacies of wound healing microenvironment for limiting single agent-elicited carcinogenesis and two-stage carcinogenesis. The results showed that three compounds used alone could promote wound healing but had unfavorable efficacy to exert wound healing, and that the combination of three compounds made up treatment disadvantage of a single compound in wound healing and led to optimal wound healing. Although individual treatment with these agents may prevent cancer, they were not effective for the treatment of established tumors. However, combination treatment with these three compounds almost completely prevented urethane-induced lung carcinogenesis and reduced tumor burden. Different from previous studies, we found that urethane-induced lung carcinogenesis was associated with lung injury independent of pulmonary inflammation. LPS-induced pulmonary inflammation did not increase lung carcinogenesis, whereas decreased pulmonary inflammation by macrophage depletion promoted lung carcinogenesis. In addition, urethane damaged wound healing in skin excision wound model, reversed lung carcinogenic efficacy by the combination of three compounds was consistent with skin wound healing. Further, the combination of these three agents reduced the number of lung cancer stem cells (CSCs) by inducing cell differentiation, restoration of gap junction intercellular communication (GJIC) and blockade of the epithelial-to-mesenchymal transition (EMT). Our results suggest that restoration of a wound healing microenvironment represents an effective strategy for cancer prevention.</p></div

    The wound healing microenvironment restored GJIC but did not directly decrease tumor cells.

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    <p><b>(</b>A) The combination of three compounds reversed GJIC loss in urethane-treated BEAS-2B cells. Images were taken at ×10 magnification. (B and C) The combination of three compounds led to angiogenesis and optimal fibroblast proliferation but did not directly decrease A549 cancer cells. The results are presented as mean±SE (n = 5/group) **<i>p</i> < 0.01 <i>vs</i> control group.</p

    PEGylated Self-Growth MoS<sub>2</sub> on a Cotton Cloth Substrate for High-Efficiency Solar Energy Utilization

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    Solar steam generation, utilizing abundant solar energy and floating photothermal materials, has been considered as one of the most sustainable, efficient ways to solve the problem of water shortage. Here, a new system for solar steam generation is fabricated based on a PEGylated MoS<sub>2</sub>-cotton cloth (PMoS<sub>2</sub>-CC). 80.5–90 ± 3.5% of high-efficiency solar steam generation is achieved under a light density of 1–5 kW m<sup>–2</sup> because of the good gas permeability of CC and the hydrophilic property of PMoS<sub>2</sub>-CC. The self-growth PMoS<sub>2</sub>-CC provides good photothermal performances in pure water and saline water. The water evaporation rate with PMoS<sub>2</sub>-CC keeps a stable value after a long-time illumination (4 h) and 32 times cycle tests. Our result provides a way to prepare pure water in the applications for alleviating a scarcity of drinking water

    The screened three wound healing agents suppressed lung carcinogenesis and their combination led to optimal preventive efficacy.

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    <p>(A) Three wound healing agents were administered to animals following the first carcinogen treatment and were found to prevent lung carcinogenesis. (B) They were administered following the last carcinogen treatment but did not prevent lung carcinogenesis, whereas the combination of these three agents remained effective. (C) Summary data of lung tumor incidence (n = 20). (D) Three wound healing agents restored the lung barrier in urethane-induced lung carcinogenesis. The results are presented as mean±SE (n = 10/group). *<i>p</i> < 0.05, **<i>p</i> < 0.01, vs control group.</p

    The wound healing microenvironment prevented lung cell malignant transformation.

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    <p>(A) The combination of three compounds reduced the population expressing stem cell markers or EMT markers examined by flow cytometry and in A549 cells. (B) The combination of three compounds decreased A549 cell self-renew shown as the number of tumor spheres and soft agar colonies. (C) The combination of three compounds decreased the population expressing EMT markers examined by flow cytometry in urethane-treated BEAS-2B cells. The results are presented as mean±SE (n = 5/group) **<i>p</i> < 0.01 <i>vs</i> control group.</p

    The combined models increased lung tumor overall volume but did not negatively impact health or significantly affect the body weights of mice.

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    <p>(A) The combined models increased lung tumor overall volume and decreased carcinogenic preventive efficacy of a single compound. (B-E) The combined models did not negatively impact health or significantly affect the body weights of mice. The results are presented as mean±SE (n = 20/group). **<i>p</i> < 0.01, vs control group.</p

    Gingerol Reverses the Cancer-Promoting Effect of Capsaicin by Increased TRPV1 Level in a Urethane-Induced Lung Carcinogenic Model

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    Both gingerol and capsaicin are agonists of TRPV1, which can negatively control tumor progression. This study observed the long-term effects of oral administration of 6-gingerol alone or in combination with capsaicin for 20 weeks in a urethane-induced lung carcinogenic model. We showed that lung carcinoma incidence and multiplicity were 70% and 21.2 ± 3.6, respectively, in the control versus 100% and 35.6 ± 5.2 in the capsaicin group (<i>P</i> < 0.01) and 50% and 10.8 ± 3.1 in the 6-gingerol group (<i>P</i> < 0.01). The combination of 6-gingerol and capsaicin reversed the cancer-promoting effect of capsaicin (carcinoma incidence of 100% versus 20% and multiplicity of 35.6 ± 5.2 versus 4.7 ± 2.3; <i>P</i> < 0.001). The cancer-promoting effect of capsaicin was due to increased epidermal growth-factor receptor (EGFR) level by decreased transient receptor potential vanilloid type-1 (TRPV1) level (<i>P</i> < 0.01) . The capsaicin-decreased EGFR level subsequently reduced levels of nuclear factor-κB (NF-κB) and cyclin D1 that favored enhanced lung epithelial proliferation and epithelial–mesenchymal transition (EMT) during lung carcinogenesis (<i>P</i> < 0.01). In contrast, 6-gingerol promoted TRPV1 level and drastically decreased the levels of EGFR, NF-κB, and cyclin D1 that favored reduced lung epithelial proliferation and EMT (<i>P</i> < 0.01). This study provides valuable information for the long-term consumption of chili-pepper-rich diets to decrease the risk of cancer development

    Facile Strategy for Electrochemical Analysis of Hydrogen Peroxide Based on Multifunctional Fe<sub>3</sub>O<sub>4</sub>@Ag Nanocomposites

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    The authors are presenting the application of Fe<sub>3</sub>O<sub>4</sub>@Ag nanocomposites to a highly sensitive and selective analysis of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). The morphology and structure of the synthesized porous Fe<sub>3</sub>O<sub>4</sub> nanoparticles and Fe<sub>3</sub>O<sub>4</sub>@Ag nanocomposites are well-characterized by scanning electron microscopy (SEM), X-ray powder diffraction (XRD), UV–vis absorption spectrum, etc. The nanocomposites can be facilely modified on the magnetic glassy carbon electrode, which also exhibit a sharp silver stripping peak owing to the loaded Ag nanoparticles. Since an autocatalytic oxidation reaction of Ag nanoparticles occurs with the existence of H<sub>2</sub>O<sub>2</sub>, the peak current intensity is suitable to track H<sub>2</sub>O<sub>2</sub> levels. The analytical performance is evaluated by the technique of linear sweep voltammetry, which shows a linear range from 0.5 to 20 μM. The limit of detection is as low as 0.16 μM. The detection can be completed in 5 min, which is quite fast and convenient. In addition, an electrochemical track of H<sub>2</sub>O<sub>2</sub> released from living cells is also achieved. Therefore, the potential applications in biological studies and disease theranostics are promised

    Urethane-induced lung carcinogenesis was associated with lung injury independent of pulmonary inflammation.

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    <p>(A) Urethane-induced lung carcinogenesis was promoted by BLM- or macrophage depletion-induced lung injury but was not affected by LPS-induced pulmonary inflammation. (B-D) LPS exposure, BLM exposure and macrophage depletion reversed carcinogenic preventive efficacy of single compound but had slight effect on combined efficacy shown as lung tumor incidence, lung tumor number and lung weight, respectively. The results are presented as mean±SE (n = 20/group). *<i>p</i> < 0.05, **<i>p</i> < 0.01, vs control group.</p

    The screened three wound healing agents desereased lung tumor overall volume and did not negatively impact health or significantly affect the body weights of mice.

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    <p>(A) Three wound healing agents desereased lung tumor overall volume. (B-D) Three wound healing agents did not negatively impact health or significantly affect the body weights of mice. The results are presented as mean±SE (n = 10/group). *<i>p</i> < 0.05, **<i>p</i> < 0.01, vs control group.</p
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