7 research outputs found

    Identification of Transcriptome-Derived Microsatellite Markers and Their Association with the Growth Performance of the Mud Crab (<i>Scylla paramamosain</i>)

    Get PDF
    <div><p>Microsatellite markers from a transcriptome sequence library were initially isolated, and their genetic variation was characterized in a wild population of the mud crab (<i>Scylla paramamosain</i>). We then tested the association between these microsatellite markers and the growth performance of <i>S. paramamosain</i>. A total of 129 polymorphic microsatellite markers were identified, with an observed heterozygosity ranging from 0.19 to 1.00 per locus, an expected heterozygosity ranging from 0.23 to 0.96 per locus, and a polymorphism information content (PIC) ranging from 0.21 to 0.95 per locus. Of these microsatellite markers, 30 showed polymorphism in 96 full-sib individuals of a first generation family. Statistical analysis indicated that three microsatellite markers were significantly associated with 12 growth traits of <i>S. paramamosain</i>. Of these three markers, locus Scpa36 was significantly associated with eight growth traits, namely, carapace length, abdomen width (AW), body height (BH), fixed finger length of the claw, fixed finger width of the claw, fixed finger height of the claw, meropodite length of pereiopod 2, and meropodite length of pereiopod 3 (MLP3) (<i>P</i><0.05). Locus Scpa75 was significantly associated with five growth traits, namely, internal carapace width, AW, carapace width at spine 8, distance between lateral spine 2 (DLS2), and MLP3 (<i>P</i><0.05). Locus Spm30 was significantly associated with BH, DLS2, and body weight (<i>P</i><0.05). Further analysis suggested a set of genotypes (BC at Scpa36, BC and BD at Scpa75, and AC at Spm30) that have great potential in the selection of <i>S. paramamosain</i> for growth traits. These findings will facilitate the development of population conservation genetics and molecular marker-assisted selective breeding of <i>S. paramamosain</i> and other closely related species.</p></div

    Association analysis between microsatellite locus Scpa75 and five growth traits of <i>S. paramamosain.</i>

    No full text
    <p>ICW, internal carapace width; AW, abdomen width; CWS8, carapace width at spine 8; DLS2, distance between lateral spine 2; MLP3, meropodite length of pereiopod 3; <sup>a, b</sup> and<sup> ab</sup>, superscripts in different genotypes without a common one are significant different (<i>P</i><0.05).</p

    Comparison of the appearance of CECT and clinical severity of AP.

    No full text
    <p>(A) The incidence of ANP in AP patients with AFL or NAFL; (B) The clinical severity of AP in AP patients with AFL or NAFL. CECT: contrast-enhanced computed tomography; AP: acute pancreatitis; AFL: alcoholic fatty liver; NAFL: non- alcoholic fatty liver; AEP: acute edematous pancreatitis; ANP: acute necrotizing pancreatitis; MAP: mild acute pancreatitis; MSAP: moderately severe acute pancreatitis; SAP: severe acute pancreatitis.</p

    Genetic variation of 30 microsatellite markers in G<sub>1</sub> family of <i>S. paramamosain.</i>

    No full text
    <p><i>N</i><sub>a</sub>, observed number of alleles; <i>N</i><sub>e</sub>, effective number of alleles; <i>H</i><sub>O</sub>, observed heterozygosity; <i>H</i><sub>E</sub>, expected heterozygosity; PIC, polymorphism information content.</p

    Comparison of the appearance of CECT and clinical severity of AP.

    No full text
    <p>(A, B) The incidence of ANP in total AP patients with or without FL; (C, D) The clinical severity of AP in total patients with or without FL; (E, F) The incidence of ANP in non-obese AP patients with or without FL; (G, H) The clinical severity of AP in non-obese patients with or without FL; (I, J) The incidence of ANP in obese AP patients with or without FL; (K, L) The clinical severity of AP in obese patients with or without FL; (B, D, F, H, J, L) FL patients were divided according to the severity of FL, including mild FL, moderate FL and severe FL. CECT: contrast-enhanced computed tomography; AP: acute pancreatitis; FL: fatty liver; AEP: acute edematous pancreatitis; ANP: acute necrotizing pancreatitis; MAP: mild acute pancreatitis; MSAP: moderately severe acute pancreatitis; SAP: severe acute pancreatitis.</p

    Association analysis between microsatellite locus Scpa36 and eight growth traits of <i>S. paramamosain.</i>

    No full text
    <p>CL, carapace length; AW, abdomen width; BH, body height; FFLC, fixed finger length of the claw; FFWC, fixed finger width of the claw; FFHC, fixed finger height of the claw; MLP2, meropodite length of pereiopod 2; MLP3, meropodite length of pereiopod 3; <sup>a, b, ab, bc</sup> and <sup>c</sup>, superscripts in different genotypes without a common one are significant different (<i>P</i><0.05).</p

    Comparison of incidence of complications and outcomes in AP patients with FL or not.

    No full text
    <p>AP: acute pancreatitis; FL: fatty liver; SIRS: systemic inflammatory response syndrome;</p><p>*p<0.05.</p><p>Comparison of incidence of complications and outcomes in AP patients with FL or not.</p
    corecore