7 research outputs found

    Magnetic Molecularly Imprinted Nanoparticles for Rapid and Selective Detection of Dimethyl Phthalate in Water Using SERS

    No full text
    The plastics industry commonly uses dimethyl phthalate (DMP) as a plasticizer. DMP is highly permeable to nature at different pH levels and temperatures, resulting in the contamination of water, soil, and air. As a result of the high cost, low selectivity, and complicated pretreatment in the DMP detection process, this paper synthesized ferromagnetic nanomaterials with molecular imprinting, simplified the pretreatment process by ferromagnetic nanomaterials, selectively adsorbed DMP using the molecular imprinting method, and finally detected DMP with the material by SERS. Molecular imprinting polymers (MIPs) have a higher affinity for DMP than NIPs, which are characterized by fast adsorption rates, strong binding ability, and improved selective adsorption ability. Furthermore, the MIPs are reusable, exhibiting only about a 7% loss in adsorption capacity after seven adsorption–desorption experiments. As a consequence of the adsorption of DMP onto Fe3O4@MIPs@Ag, DMP detection was achieved through SERS characterization, and it was found that the DMP concentration was linearly related to the intensity of the corresponding characteristic peak associated with the DMP, with a detection limit of 4.2 × 10–11 M. According to the tested water samples, the recovery rating ranged from 92.6 to 105%, demonstrating the feasibility of the proposed method for the detection of DMP in real water samples

    LncRNA GAS5 promoted cell proliferation, cell cycle and inhibited cell apoptosis in PCa cells.

    No full text
    <p>(A) The efficiency of siGAS5 was confirmed by qRT-PCR. (B) Knockdown of GAS5 inhibited the proliferation of LNCaP cells. (C) Cell cycle assay was performed in LNCaP cells. Cells were transfected with NC or siGAS5, stained with PI and evaluated with FACScalibur flow cytometer. Knockdown of GAS5 inhibited cell cycle. (D) Cell apoptosis assay was performed in PC-3 cells. Cells were transfected with si-NC or si-GAS5, stained with PI and FITC. GAS5 knockdown increased the percentage of cells in both early apoptosis and late apoptosis. The cell cycle and apoptosis analysis results presented as mean ± SD (n = 3). Significance was defined as p < 0.05 (*p < 0.05; **p < 0.01; ***p < 0.001).</p