110 research outputs found

    Image_1_LASSO-derived prognostic model predicts cancer-specific survival in advanced pancreatic ductal adenocarcinoma over 50 years of age: a retrospective study of SEER database research.tif

    No full text
    BackgroundThis study aimed to develop a prognostic model for patients with advanced ductal adenocarcinoma aged ≥50 years.MethodsPatient information was extracted from the Surveillance, Epidemiology, and End Results (SEER) database. Least absolute shrinkage and selection operator (LASSO) Cox regression analysis was performed to screen the model variables. Cases from Nanchang Central Hospital were collected for external validation. The new nomogram and the American Joint Committee on Cancer (AJCC) criteria were evaluated using integrated discrimination improvement (IDI) and net reclassification index (NRI) indicators. Survival curves presented the prognosis of the new classification system and AJCC criteria.ResultsIn total, 17,621 eligible patients were included. Lasso Cox regression selected 4 variables including age, chemotherapy, radiotherapy and AJCC stage. The C-index of the training cohort was 0.721. The C-index value of the validation cohort was 0.729. The AUCs for the training cohorts at 1, 2, and 3 years were 0.749, 0.729, and 0.715, respectively. The calibration curves showed that the predicted and actual probabilities at 1, 2, and 3 years matched. External validation confirmed the model’s outstanding predictive power. Decision curve analysis indicated that the clinical benefit of the nomogram was higher than that of the AJCC staging system. The model evaluation indices preceded the AJCC staging with NRI (1-year: 0.88, 2-year: 0.94, 3-year: 0.72) and IDI (1-year: 0.24, 2-year: 0.23, 3-year: 0.22). The Kaplan–Meier curves implied that the new classification system was more capable of distinguishing between patients at different risks.ConclusionsThis study established a prognostic nomogram and risk classification system for advanced pancreatic cancer in patients aged ≥50 years to provide a practical tool for the clinical management of patients with pancreatic ductal adenocarcinoma.</p

    Creation of Room-Temperature Sub-100 nm Antiferromagnetic Skyrmions in an Antiferromagnet IrMn through Interfacial Exchange Coupling

    No full text
    Antiferromagnetic (AFM) skyrmions are magnetic vortices composed of antiparallell-aligned neighboring spins. In stark contrast to conventional skyrmions based on ferromagnetic order, AFM skyrmions have vanished stray fields, higher response frequencies, and rectified translational motion driven by an external force. Therefore, AFM skyrmions promise highly efficient spintronics devices with high bit mobility and density. Nevertheless, the experimental realization of intrinsic AFM skyrmions remains elusive. Here, we show that AFM skyrmions can be nucleated via interfacial exchange coupling at the surface of a room-temperature AFM material, IrMn, exploiting the particular response from uncompensated moments to the thermal annealing and imprinting effects. Further systematic magnetic characterizations validate the existence of such an AFM order at the IrMn/CoFeB interfaces. Such AFM skyrmions have a typical size of 100 nm, which presents pronounced robustness against field and temperature. Our work opens new pathways for magnetic topological devices based on AFM skyrmions

    Powerful Orbital Hybridization of Copper–Silver Bimetallic Nanosheets for Electrocatalytic Nitrogen Reduction to Ammonia

    No full text
    Electrochemical nitrogen reduction (eNRR) is a promising strategy to replace the energy- and capital-intensive Haber–Bosch process. Unfortunately, the low selectivity of the eNRR process impedes the industrial application of this approach. In this work, a highly efficient and stable NRR electrocatalyst is obtained via coreduction of Cu and Ag precursors using the holly leaves as reducing agents. The as-obtained Cu3Ag bimetallic nanosheets exhibit excellent NRR performance with an NH3 production rate of 31.3 μg h–1 mg–1cat. and a Faradaic efficiency of 31.3% at −0.2 V vs RHE. According to density functional theory (DFT) calculation, the outstanding performance of Cu3Ag bimetallic nanosheets could be caused by the fact that Ag optimizes the 3d orbital occupation of Cu and synergistically enhances the charge transfer during the NRR process, resulting in a suitable adsorption strength of the intermediates

    Crashworthiness of nested corrugation square energy-absorbing tubes with circumferentially cosine profile

    No full text
    With the development of railway technology, high-speed trains have been invested in practical operation increasingly. The traffic accident that train runs at high speed will cause casualties and property losses. The energy absorption tube can effectively alleviate the damage of the vehicle in the collision process and protect passengers in the buffering process. In this paper, nested corrugation square energy-absorbing tubes (NCSTs) with circumferentially cosine profile and different inner structures are analyzed by adopting finite element (FE) simulation. The quasi-static compression tests and proposed theoretical model based on Simplified Super Folding Element (SSFE) theory are employed to validate the effectiveness of numerical models. The simulation results indicates that the crushing modes possess certain sensitiveness to cross-sectional conformation and the NCSTs present more stable and orderly deformation modes compared with non-nested tubes. The specific energy absorption (SEA) and crushing force efficiency (CFE) of NCST can be increased by 52.49 and 19.07% compared to ordinary corrugation tube, respectively. In addition, the tube with two-layers octagonal wall (SO1D) is selected as optimal structure because it is ranked first under cases of 40% by adopting the technique for order preference by similarity to ideal solution (TOPSIS) method. Finally, the sensitivity analyses based on SO1D are conducted. The results show that the parametric variation of trib and tout fairly effect on the deformation pattern. And increases in wall thickness t and section size of the middle wall Dmid will all result to increases in initial peak crushing force (IPCF) and significant variation on other crashworthiness criteria. This paper offers certain reference significance for the study of energy-absorbing structures.</p

    Flow and fracture behavior of aluminum alloy 6082-T6 at different tensile strain rates and triaxialities - Fig 3

    No full text
    <p>(a) Split Hopkinson pressure bar (SHTB) testing apparatus; (b) The geometry of the SHTB-specimen; (c) A notched specimen sandwiched between the incident bar and the transmission bar.</p

    Identification of odorant binding proteins and chemosensory proteins in <i>Microplitis mediator</i> as well as functional characterization of chemosensory protein 3

    No full text
    <div><p>Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play important roles in transporting semiochemicals through the sensillar lymph to olfactory receptors in insect antennae. In the present study, twenty OBPs and three CSPs were identified from the antennal transcriptome of <i>Microplitis mediator</i>. Ten OBPs (<i>MmedOBP11–20</i>) and two CSPs (<i>MmedCSP2–3</i>) were newly identified. The expression patterns of these new genes in olfactory and non-olfactory tissues were investigated by real-time quantitative PCR (qPCR) measurement. The results indicated that <i>MmedOBP14</i>, <i>MmedOBP18</i>, <i>MmedCSP2</i> and <i>MmedCSP3</i> were primarily expressed in antennae suggesting potential olfactory roles in <i>M</i>. <i>mediator</i>. However, other genes including <i>MmedOBP11</i>–<i>13</i>, <i>15–17</i>, <i>19</i>–<i>20</i> appeared to be expressed at higher levels in body parts than in antennae. Focusing on the functional characterization of MmedCSP3, immunocytochemistry and fluorescent competitive binding assays were conducted indoors. It was found that MmedCSP3 was specifically located in the sensillum lymph of olfactory sensilla basiconca type 2. The recombinant MmedCSP3 could bind several types of host insects odors and plant volatiles. Interestingly, three sex pheromone components of Noctuidae insects, <i>cis</i>-11-hexadecenyl aldehyde (<i>Z</i>11-16: Ald), <i>cis</i>-11-hexadecanol (<i>Z</i>11-16: OH), and <i>trans</i>-11-tetradecenyl acetate (<i>E</i>11-14: Ac), showed high binding affinities (Ki = 17.24–18.77 μM). The MmedCSP3 may be involved in locating host insects. Our data provide a base for further investigating the physiological roles of OBPs and CSPs in <i>M</i>. <i>mediator</i>, and extend the function of MmedCSP3 in chemoreception of <i>M</i>. <i>mediator</i>.</p></div

    Direct Observation of Magnetocrystalline Anisotropy Tuning Magnetization Configurations in Uniaxial Magnetic Nanomaterials

    Get PDF
    Discovering the effect of magnetic anisotropy on the magnetization configurations of magnetic nanomaterials is essential and significant for not only enriching the fundamental knowledge of magnetics but also facilitating the designs of desired magnetic nanostructures for diverse technological applications, such as data storage devices, spintronic devices, and magnetic nanosensors. Herein, we present a direct observation of magnetocrystalline anisotropy tuning magnetization configurations in uniaxial magnetic nanomaterials with hexagonal structure by means of three modeled samples. The magnetic configuration in polycrystalline BaFe<sub>12</sub>O<sub>19</sub> nanoslice is a curling structure, revealing that the effect of magnetocrystalline anisotropy in uniaxial magnetic nanomaterials can be broken by forming an amorphous structure or polycrystalline structure with tiny grains. Both single crystalline BaFe<sub>12</sub>O<sub>19</sub> nanoslice and individual particles of single-particle-chain BaFe<sub>12</sub>O<sub>19</sub> nanowire appear in a single domain state, revealing a dominant role of magnetocrystalline anisotropy in the magnetization configuration of uniaxial magnetic nanomaterials. These observations are further verified by micromagnetic computational simulations

    SDS-PAGE and western blot analysis of recombinant MmedCSP3.

    No full text
    <p>M: Molecular weight marker; 1: Non-induced pET-30a (+) / MmedCSP3; 2: Induced pET-30a (+) / MmedCSP3; 3: pET-30a (+) / MmedCSP3 supernatant; 4: pET-30a (+) / MmedCSP3 pellet; 5: Purified MmedCSP3 with His-tag; 6: Purified MmedCSP3 without His-tag; 7: Western blot analysis of MmedCSP3.</p

    PD1/PD-L1 signaling sustains the survival and proliferation of cisplatin resistant cells.

    No full text
    <p>A, Resistant and parental H69 cells were treated with indicated doses of cisplatin and subjected to CCK8 assays. Note: CR, Cisplatin resistance. B, The H69R and H82R cells were growing in drug-free medium for 72 hours. The qPCR or Western blot was used to measure the RNA (left panel) or protein (right panel) expression of PD1 or PD-L1. Data represent three independent experiments, and are the mean ±SD, *<i>P</i> < 0.05. Note: PA, Parental; CR, Cisplatin resistance. C, H69R and H82R cells were transfected with PD-L1 shRNA or control vectors for 24 hours, and then exposed to 3 μM of cisplatin for additional 24 hours. Western blot (left panel) was used to measure PD-L1 protein expression, but CCK8 assays for the cell proliferation. Note: Con, Control vectors; shP, PD-L1 shRNA; Cis, Cisplatin. In CCK8 assays, the experiments are done two times independently with 8 replicates. *<i>P</i> < 0.05, **<i>P</i> < 0.01.</p