459 research outputs found

    Analysis of Chlorogenic Acid Oxidation Pathway in Simulated Enzymatic Honeysuckle Browning System by High Performance Liquid Chromatography and Mass Spectrometry

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    Purpose: To investigate the pathways involved in the oxidation of chlorogenic acid (CA) and phenol metabolism in honeysuckle buds.Methods: A model that mimics CA oxidation by honeysuckle polyphenol oxidase (PPO) by controlling the reaction temperature or reaction duration was employed, and the resulting products were analyzed by high-performance liquid chromatography followed by mass spectrometry (LC-MS).Results: The pathway of CA oxidation by PPO in honeysuckle involves CA, hydroxyl - CA, CA-oquinone derivatives, and CA-p-quinone derivatives.Conclusion: CA oxidation and the enzymatic browning reaction significantly impact the quality of honeysuckle and other fresh agricultural products.Keywords: Honeysuckle, Chlorogenic acid, Enzymatic browning, Mimic system, Oxidation pathway, High-performance liquid chromatography-mass spectrometr

    Intestinal Microecology in Pediatric Surgery-Related Gastrointestinal Diseases Current Insights and Future Perspectives

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    Intestinal microecology is established from birth and is constantly changing until homeostasis is reached. Intestinal microecology is involved in the immune inflammatory response of the intestine and regulates the intestinal barrier function. The imbalance of intestinal microecology is closely related to the occurrence and development of digestive system diseases. In some gastrointestinal diseases related to pediatric surgery, intestinal microecology and its metabolites undergo a series of changes, which can provide a certain basis for the diagnosis of diseases. The continuous development of microecological agents and fecal microbiota transplantation technology has provided a new means for its clinical treatment. We review the relationship between pathogenesis, diagnosis and treatment of pediatric surgery-related gastrointestinal diseases and intestinal microecology, in order to provide new ideas and methods for clinical diagnosis, treatment and research
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