12 research outputs found

    Bimodal Electrochemiluminescence of G‑CNQDs in the Presence of Double Coreactants for Ascorbic Acid Detection

    No full text
    How to improve the accuracy of target detection substance in low-content and complex of real sample, which is still a major challenge in the analysis field. There is no doubt that the internal standard method is the best choice in the analysis methods. The internal standard method of ECL strategy can furnish more accurate detection results in the changeable complex environment, and it can dispel the primary vaguest interference in the system through the self-calibration of two emission spectra. Herein, we effectually explored a strong and stable bimodal ECL system based on graphitic carbon nitride quantum dots (g-CNQDs) as single luminophore in the presence of double coreactants potassium persulfate (K<sub>2</sub>S<sub>2</sub>O<sub>8</sub>) and tetrabutylammonium bromide (TBAB) under the optimized conditions. ECL-1 at 2.82 V and ECL-2 at 1.73 V were observed when the potential was scanned between −3 and 3 V at the scan rate of 0.2 V·s<sup>–1</sup>. The ECL-1 was responding to the analyte, that is, ascorbic acid (AA) and the ECL-2 was not for a certain concentration of AA; hence, the developed bimodal ECL system was used as internal standard method for quantitative AA in human serum due to the different sensitivity of the double-peak ECL signals to the target analytes. The linear relationships were obtained based on the ln <i>I</i> (ECL-1/ECL-2) against the concentration of AA in the concentration range of 3.5 to 330 nM, with a detection limit of 110 pM (S/N = 3)

    Synthesis and characterization of a 2, 5-Bis(trimethylsilyl)-substituted Bis(1, 1′-silolide) dianion

    No full text
    <p>A novel silyl-substituted bis(1, 1′-silolide) dianion has been isolated and characterized by single-crystal X-ray analysis for the first time. The <sup>1</sup>H, <sup>29</sup>Si, and <sup>13</sup>C NMR spectra show significant delocalization of the negative charges to the silole ring. X-ray crystallography revealed equalized C−C distances and DFT calculation also indicates for significant aromaticity. Reaction with trimethylchlorosilane gave the expected bis(1, 2, 5-tris(trimethylsilyl)-3, 4-diphenyl-silacyclopentadienyl).</p

    The Combination of Three Natural Compounds Effectively Prevented Lung Carcinogenesis by Optimal Wound Healing

    No full text
    <div><p>The tumor stroma has been described as “normal wound healing gone awry”. We explored whether the restoration of a wound healing-like microenvironment may facilitate tumor healing. Firstly, we screened three natural compounds (shikonin, notoginsenoside R1 and aconitine) from wound healing agents and evaluated the efficacies of wound healing microenvironment for limiting single agent-elicited carcinogenesis and two-stage carcinogenesis. The results showed that three compounds used alone could promote wound healing but had unfavorable efficacy to exert wound healing, and that the combination of three compounds made up treatment disadvantage of a single compound in wound healing and led to optimal wound healing. Although individual treatment with these agents may prevent cancer, they were not effective for the treatment of established tumors. However, combination treatment with these three compounds almost completely prevented urethane-induced lung carcinogenesis and reduced tumor burden. Different from previous studies, we found that urethane-induced lung carcinogenesis was associated with lung injury independent of pulmonary inflammation. LPS-induced pulmonary inflammation did not increase lung carcinogenesis, whereas decreased pulmonary inflammation by macrophage depletion promoted lung carcinogenesis. In addition, urethane damaged wound healing in skin excision wound model, reversed lung carcinogenic efficacy by the combination of three compounds was consistent with skin wound healing. Further, the combination of these three agents reduced the number of lung cancer stem cells (CSCs) by inducing cell differentiation, restoration of gap junction intercellular communication (GJIC) and blockade of the epithelial-to-mesenchymal transition (EMT). Our results suggest that restoration of a wound healing microenvironment represents an effective strategy for cancer prevention.</p></div

    The wound healing microenvironment restored GJIC but did not directly decrease tumor cells.

    No full text
    <p><b>(</b>A) The combination of three compounds reversed GJIC loss in urethane-treated BEAS-2B cells. Images were taken at ×10 magnification. (B and C) The combination of three compounds led to angiogenesis and optimal fibroblast proliferation but did not directly decrease A549 cancer cells. The results are presented as mean±SE (n = 5/group) **<i>p</i> < 0.01 <i>vs</i> control group.</p

    The screened three wound healing agents suppressed lung carcinogenesis and their combination led to optimal preventive efficacy.

    No full text
    <p>(A) Three wound healing agents were administered to animals following the first carcinogen treatment and were found to prevent lung carcinogenesis. (B) They were administered following the last carcinogen treatment but did not prevent lung carcinogenesis, whereas the combination of these three agents remained effective. (C) Summary data of lung tumor incidence (n = 20). (D) Three wound healing agents restored the lung barrier in urethane-induced lung carcinogenesis. The results are presented as mean±SE (n = 10/group). *<i>p</i> < 0.05, **<i>p</i> < 0.01, vs control group.</p

    Genetic diversity and evolution of <i>Apple stem pitting virus</i> isolates from pear in China

    No full text
    <p>To determine the population structure and mechanisms of molecular evolution of <i>Apple stem pitting virus</i> (ASPV) isolates from pear in China, we compared 48 coat protein (CP) sequences from 31 ASPV pear isolates and 66 Triple Gene Block (TGB) sequences from 44 ASPV pear isolates. Phylogenetic analysis based on these sequences and corresponding sequences from GenBank showed that ASPV grouping in phylogenetic trees was correlated to the host of origin (apple, pear and Korla pear), regardless of gene sequences examined. The ASPV isolates from pear could be divided into six evolutionary divergent subgroups (A–F) based on their CP sequences, and two new subgroups (B and F) were identified in this study. The ASPV isolates could be divided into five evolutionarily divergent groups based on their TGB sequences. Multiple alignment analysis indicated continuous nucleotide insertions or deletions were present in CP of ASPV pear isolates in China. Recombination events were detected in CP and TGB sequences in our study. These results suggest that ASPV CP and TGB genes were under negative selection. Our study suggests that insertion or deletion mutation, selection pressure and recombination play important roles in genetic diversity of ASPV pear isolates in China.</p

    The wound healing microenvironment prevented lung cell malignant transformation.

    No full text
    <p>(A) The combination of three compounds reduced the population expressing stem cell markers or EMT markers examined by flow cytometry and in A549 cells. (B) The combination of three compounds decreased A549 cell self-renew shown as the number of tumor spheres and soft agar colonies. (C) The combination of three compounds decreased the population expressing EMT markers examined by flow cytometry in urethane-treated BEAS-2B cells. The results are presented as mean±SE (n = 5/group) **<i>p</i> < 0.01 <i>vs</i> control group.</p

    The combined models increased lung tumor overall volume but did not negatively impact health or significantly affect the body weights of mice.

    No full text
    <p>(A) The combined models increased lung tumor overall volume and decreased carcinogenic preventive efficacy of a single compound. (B-E) The combined models did not negatively impact health or significantly affect the body weights of mice. The results are presented as mean±SE (n = 20/group). **<i>p</i> < 0.01, vs control group.</p

    Gingerol Reverses the Cancer-Promoting Effect of Capsaicin by Increased TRPV1 Level in a Urethane-Induced Lung Carcinogenic Model

    No full text
    Both gingerol and capsaicin are agonists of TRPV1, which can negatively control tumor progression. This study observed the long-term effects of oral administration of 6-gingerol alone or in combination with capsaicin for 20 weeks in a urethane-induced lung carcinogenic model. We showed that lung carcinoma incidence and multiplicity were 70% and 21.2 ± 3.6, respectively, in the control versus 100% and 35.6 ± 5.2 in the capsaicin group (<i>P</i> < 0.01) and 50% and 10.8 ± 3.1 in the 6-gingerol group (<i>P</i> < 0.01). The combination of 6-gingerol and capsaicin reversed the cancer-promoting effect of capsaicin (carcinoma incidence of 100% versus 20% and multiplicity of 35.6 ± 5.2 versus 4.7 ± 2.3; <i>P</i> < 0.001). The cancer-promoting effect of capsaicin was due to increased epidermal growth-factor receptor (EGFR) level by decreased transient receptor potential vanilloid type-1 (TRPV1) level (<i>P</i> < 0.01) . The capsaicin-decreased EGFR level subsequently reduced levels of nuclear factor-κB (NF-κB) and cyclin D1 that favored enhanced lung epithelial proliferation and epithelial–mesenchymal transition (EMT) during lung carcinogenesis (<i>P</i> < 0.01). In contrast, 6-gingerol promoted TRPV1 level and drastically decreased the levels of EGFR, NF-κB, and cyclin D1 that favored reduced lung epithelial proliferation and EMT (<i>P</i> < 0.01). This study provides valuable information for the long-term consumption of chili-pepper-rich diets to decrease the risk of cancer development

    Urethane-induced lung carcinogenesis was associated with lung injury independent of pulmonary inflammation.

    No full text
    <p>(A) Urethane-induced lung carcinogenesis was promoted by BLM- or macrophage depletion-induced lung injury but was not affected by LPS-induced pulmonary inflammation. (B-D) LPS exposure, BLM exposure and macrophage depletion reversed carcinogenic preventive efficacy of single compound but had slight effect on combined efficacy shown as lung tumor incidence, lung tumor number and lung weight, respectively. The results are presented as mean±SE (n = 20/group). *<i>p</i> < 0.05, **<i>p</i> < 0.01, vs control group.</p
    corecore