15 research outputs found

    Blood serum metabolome of atopic dermatitis: Altered energy cycle and the markers of systemic inflammation - Fig 4

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    <p><b>PCA plots based only on metabolites that were found to be significant in untargeted analysis for positive (A) and combined (B) datasets.</b> Red circles—cases; blue squares—controls. Points on both plots are visually separable and form very clear clusters that correlate with phenotypes.</p

    E-Cigarette Affects the Metabolome of Primary Normal Human Bronchial Epithelial Cells

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    <div><p>E-cigarettes are widely believed to be safer than conventional cigarettes and have been even suggested as aids for smoking cessation. However, while reasonable with some regards, this judgment is not yet supported by adequate biomedical research data. Since bronchial epithelial cells are the immediate target of inhaled toxicants, we hypothesized that exposure to e-cigarettes may affect the metabolome of human bronchial epithelial cells (HBEC) and that the changes are, at least in part, induced by oxidant-driven mechanisms. Therefore, we evaluated the effect of e-cigarette liquid (ECL) on the metabolome of HBEC and examined the potency of antioxidants to protect the cells. We assessed the changes of the intracellular metabolome upon treatment with ECL in comparison of the effect of cigarette smoke condensate (CSC) with mass spectrometry and principal component analysis on air-liquid interface model of normal HBEC. Thereafter, we evaluated the capability of the novel antioxidant tetrapeptide O-methyl-l-tyrosinyl-γ-l-glutamyl-l-cysteinylglycine (UPF1) to attenuate the effect of ECL. ECL caused a significant shift in the metabolome that gradually gained its maximum by the 5<sup>th</sup> hour and receded by the 7<sup>th</sup> hour. A second alteration followed at the 13<sup>th</sup> hour. Treatment with CSC caused a significant initial shift already by the 1<sup>st</sup> hour. ECL, but not CSC, significantly increased the concentrations of arginine, histidine, and xanthine. ECL, in parallel with CSC, increased the content of adenosine diphosphate and decreased that of three lipid species from the phosphatidylcholine family. UPF1 partially counteracted the ECL-induced deviations, UPF1’s maximum effect occurred at the 5<sup>th</sup> hour. The data support our hypothesis that ECL profoundly alters the metabolome of HBEC in a manner, which is comparable and partially overlapping with the effect of CSC. Hence, our results do not support the concept of harmlessness of e-cigarettes.</p></div

    Characteristics of the individuals involved in the measurement of poly(ADP-ribose) polymerase (PARP)-1 mRNA expression in peripheral blood mononuclear cells.

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    <p>Data are presented as mean ± SEM or n (%). <sup>*</sup>To test the equality of the data across the study groups, Kruskal-Wallis test and Pearson’s chi-square test was applied for numeric and nominal variables, respectively; in case of smoking-related variables, non-smoking controls were omitted from the analysis.</p

    Mass spectrum of primary normal human bronchial epithelial cells cultivated in air-liquid interface after exposure to e-cigarette liquid (ECL) (100 ÎĽM by nicotine) and 10 ÎĽg/mL cigarette smoke condensate (CSC) consisting of 1,822 distinct mass-to-charge signals.

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    <p>The figure represents the proportions of the spectrum that were significantly (p<i><</i>0.05) affected by addition of ECL (392 signals) or CSC (569 signals) during the first 7 h. There were 138 signals that were significantly affected by both stimuli.</p

    PCA plot for targeted analysis based on the whole data.

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    <p>Red circles—cases; blue squares—controls. Initially, both groups largely overlap, also PCs explain a modest amount of variance.</p

    Principal component analysis of the metabolic state of primary normal human bronchial epithelial cells cultivated in air-liquid interface after exposure to (A) e-cigarette liquid (ECL) (100 ÎĽM by nicotine) and (B) 10 ÎĽg/mL cigarette smoke condensate (CSC) (black solid lines).

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    <p>The time points at the arrow turns are 1, 2, 5, 7, and 13 h. Long dashes: Addition of 10 μM O-methyl-l-tyrosinyl-γ-l-glutamyl-l-cysteinylglycine to the cells having been exposed to ECL for 1 h (the arrow starts at 2 h time point), short dashes: Addition of 2 mM N-acetylcysteine to the cells having been exposed to ECL for 1 h (the arrow starts at 2 h time point). For each treatment and for any time point, n = 3 (gray circles). Error bars indicate standard errors of means. PComp1 and PComp2 are the two principal components describing the highest fraction of the effect of cells’ metabolome.</p
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