7 research outputs found

    Deleterious effect of short-term gavage of an ethanol extract of cogon grass (Imperata cylindrica L.) roots on testis and epididymal sperm quality

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    Background and Aim: Cogon grass (Imperata cylindrica L.) (CGG) is a herbal medicine that could be developed into a male antifertility agent. The present study aims to determine the effect of an ethanol extract of CGG roots on mice testicular activity, reproductive hormone levels, and epididymal sperm quality. Materials and Methods: This study was designed as completely randomized with three different doses, such as an ethanol extract of CGG roots at 0 (control), 90, and 115 mg/kg body weight. In total, 21 male DDY mice strain were treated with the CGG extract (by gavage) for 14 days, followed by an evaluation of reproductive organs, epididymal sperm quality, testis histology, histomorphometry, and reproductive hormone assays. All quantitative data were analyzed by analysis of variance, followed by Tukey's post hoc test at α=0.05. Results: The results showed that the administration of the CGG root ethanol extract disrupted the testis interstitial area and seminiferous tubules, resulting in decreased epididymal sperm quality as well as serum testosterone levels in a dose-dependent pattern. Conclusion: Oral administration of a CGG root ethanol extract induced testicular damage, decreased epididymal sperm quality, and impaired testosterone secretion

    Kualitas, Kemampuan Implantasi dan Viabilitas in-vivo Embrio Mencit (Mus muculus) Galur Swiss Webster Setelah Pembekuan Dengan Metode Vitrifikasi

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    Reproductive technologies including in vitro fertilization (IVF), embryo manipulation, gamete and embryo freezing, thawing and embryo transfer were rapidly developed. Vitrification is an embryo freezing technique that is the most developed. In this experiment, we vitrified mouse embryos and then examined the embryos i.e: (i) the quality of the embryos after thawing, (ii) the implantation rate of the embryos and (iii) viability of the embryos in vivo. Morulae and blastocycsts were collected from female mice that were pregnant a day 3,5. The embryos were equilibraten in mPBS +10% etilene glycol. Vitrification was carried out by using VABEDS medium, containing 6-10 embryos that were dropped into a tip of a straw, then frozen in liquid nitrogen for 24 hours. Thawing was carried out by flushing the embryos using mPBS suplemented with 0.5, 0.25, 0.1 and 0 M sucrose. After being incubated in M2 medium at 37oC for 1-2 hours, the recovery embryos were then transferred into the uteri of day 2.5 of pseudopregnat females. The females were then sacrificed at day 16 of gestation and the total implantaion, total life and death fetuses, as well as resorpted embryos, were taken as data. The results showed that vitrification significantly (p<0,05) reduced the quality of the embryos, as well as their implantation rate and the viability of the fetuses, which may be caused by the unoptimal combination of the cryoprotectant in the vitrification medium, temperature and exposure time during vitrification

    Protein yang Terkait dengan Teratogenisitas Anggota Tubuh Mencit Swiss Webster Akibat Perlakuan dengan Asam Metoksiasetat (MAA)

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    Telah diteliti protein yang terkait dengan teratogenesis anggota tubuh mencit Swiss Webster akibat perlakuan dengan MAA. Mencit umur kebuntingan 11 hari diberi perlakuan dosis tunggal MAA 10 mmol/kg berat badan secara gavage, sedangkan kelompok kontrol hanya diberi pelarut akuabides steril. Mencit bunting dibunuh secara dislokasi leher 4 jam setelah perlakuan dengan MAA. Tunas anggota tubuh depan diisolasi dari kelompok kontrol dan perlakuan lalu dihomogenisasi. Ekstrak kasar kemudian difraksinasi dengan amonium sulfat dan masing-masing fraksi dianalisis dengan teknik l-D dan 2-D SDS-PAGE. Elektroforegram l-D dan 2-D menunjukkan bahwa pada kelompok perlakuan fraksi protein ammonium sulfat 20-40 % (F-lI), dapat dideteksi protein 31,0-36,5 kDa serta bercak protein 35,1 kDa, pI 6,2 yang tidak terdapat pada kontrol. Pada kelompok perlakuan fraksi protein ammonium sulfat 40-60% (F-lll), dapat dideteksi protein 66,3-97,4 kDa serta bercak protein 8 I,7 kDa, pl 7,3 yang tidak terdapat pada kontrol. Sedangkan pada kelompok kontrol F-llI, protein 36,5-55,4 kDa sertab ercakp rotein 41,6 kDa, pI 6,4 terdeteksi, tetapi tidak terdeteksi pada kelompok perlakuan. Dari penelitian ini dapat disimpulkan bahwa pada tunas anggota tubuh depan mencit, perlakuan dengan MAA menginduksi ekspresi dua protein(35,1 kDa, pl 6,2 dan 81,7 kDa, pl 7,3) dan menghambat ekspresi satu protein(41,6 kDa, pl 6,4). Proteins which are Linked with Swiss Webster Mouse Limb Teratogenesis as the Effects of Methoxyacetic Acid (MAA) TreatmentThe analysis of proteins, which are linked with limb teratogenesis as the effects of MAA treated in Swiss Webster mouse has been investigated. A single dose of MAA 10 mmol/kg body weight was given by gavage on gestation day 11 , whereas the control group were administered sterilized distilled water. Pregnant mice were sacrificed by cervical dislocation at 4 hours after MAA treatment. The forelimb buds were isolated from both control and treated group embryos and were then homogenized. The crude extracts were Then fractionated with ammonium sulfate and each fraction was analyzed by 1-D and 2-D SDS-PAGE techniques respectively. The l-D and 2 -D electrophoregrams revealed that in the treated group of protein fraction 20-40% ammonium sulfate ( F-II), a protein of 31.0-36.5 kDa and a protein spot 35.1 kDa, pl 6.2 could be detected, which was not found in the control. In the treated group of protein fraction 40-60% ammonium sulfate( F-lll) a protein of 66.3-97.4 kDa and a protein spot 81.7 kDa, pl 7.3 could be detected which was not found in the control, whereas in the control group a protein of 36.5-55.4 kDa, which is a protein spot4 1.6 and p l 6.4, was detected but not detected in the treated group.It could be concluded from this experiment that in the mouse forelimb buds, MAA treatment induce the protein expression of two proteins(35.1 kDa, pl 6.2 and 81.7 kDa, 7.3) and inhibit the expression of one protein (41.6p, l 6.4)

    Perbandingan Analisa Perkerasan Metode Bina Marga Revisi Juni 2017 dan AASHTO 1993 (Studi Kasus pada Pekerjaan Rencana Preservasi Ruas Jalan Jatibarang-Langut TA 2017)

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    ABSTRAKJalan raya merupakan salah satu prasarana transportasi di Indonesia yang sering digunakan untuk menunjang kegiatan perekonomian khususnya pada jalan nasional. Namun, seringkali di jalan nasional terdapat kerusakan-kerusakan pada perkerasan jalan yang membuat kenyamanan pengendara terganggu. Salah satu ruas jalan nasional tersebut adalah jalan Jatibarang-Langut yang berada di jalur Pantura Jawa Barat. Pengujian yang dilakukan untuk mengetahui penyebab kerusakan perkerasan jalan tersebut yaitu survei traffic counting dan pengujian lendutan dengan alat Falling Weight Deflectometer (FWD) yang dalam perencanaan perhitungan tebal perkerasan akan dibandingkan menggunakan metode Bina Marga Revisi Juni 2017 dan AASHTO 1993. Data sekunder yang diperlukan adalah data daya dukung tanah dasar. Data primer yang diperoleh yaitu volume lalu lintas dan pengujian lendutan. Hasil perhitungan modulus tanah dasar 34,34 MPa dan modulus perkerasan 1.806,80 MPa. Sedangkan hasil perhitungan tebal perkerasan dengan CESA metode Bina Marga Revisi Juni 2017 47,42 cm dan CESA AASHTO 1993 38,74 cm.Kata kunci: Jatibarang-Langut, survei traffic counting, Falling Weight Deflectometer (FWD), Bina Marga Revisi Juni 2017, AASHTO 1993. ABSTRACTThe highway is one of the transportastion infrastructure in Indonesia which is often used to support economy activities especially on national road. However, often on the national road there are damages on pavement that makes disturbed rider’s comfort. The one of the national road segment is road Jatibarang-Langut located on the path Pantura, west Java. The testing done determine the cause of the pavement damege is survey traffic counting and deflection testing with Falling Weight Deflectometer (FWD) in planning pavement thickness calculation will be compared by using Bina Marga method Revision June 2017 and AASHTO 1993. The secondary data required is ground carrying capacity data. The primary data required os traffic volume and deflection testing. The calculation results of the basic soil modulus 34,34 MPa and pavement modulus 1.806,80 MPa. While the results of pavement thickness calculation by CESA method Bina Marga Revision June 2017 47,42 cm dand CESA AASHTO 1993 38,74 cm.Keywords: Jatibarang-Langut, survey traffic counting, Falling Weight Deflectometer (FWD), Bina Marga Revision June 2017, AASHTO 1993

    A teratoproteomics analysis: heat shock protein 70 is upregulated in mouse forelimb bud by methoxyacetic acid treatment

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    Methoxyacetic acid (MAA) causes fetal limb abnormalities when the substance is administrated on gestation day (GD) 11 in mice. Limb abnormalities are caused mainly by extensive cell death in the mesoderm of the limb plate. This investigation focused on identifying a protein that is linked with mouse limb teratogenicity.A single dose of MAA at 10 mmol/kg body weight was administered by gavage on GD 11; controls were administered vehicle only. Dams were killed by cervical dislocation 4 hr after treatment and forelimb buds were isolated from both the control and treated embryos. Proteins in forelimb buds GD 11 + 4 hr were precipitated out using 40-60% ammonium sulfate and were then analyzed by 2D SDS-PAGE. Excised protein spots were identified by mass spectrometry and amino acid internal sequence analysis. Identified protein was further confirmed by Western blotting.Two-dimensional gel analysis indicated that 1 protein spot of 81.7 kDa/pI 7.3 was overexpressed, and the protein matched heat shock protein 70 (HSP70; accession no. P08109, SwissProt).The results suggest that MAA, when administered to pregnant mice, upregulates HSP70 in the forelimb buds

    A teratoproteomics analysis: heat shock protein 70 is upregulated in mouse forelimb bud by methoxyacetic acid treatment

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    BACKGROUND: Methoxyacetic acid (MAA) causes fetal limb abnormalities when the substance is administrated on gestation day (GD) 11 in mice. Limb abnormalities are caused mainly by extensive cell death in the mesoderm of the limb plate. This investigation focused on identifying a protein that is linked with mouse limb teratogenicity. METHODS: A single dose of MAA at 10 mmol/kg body weight was administered by gavage on GD 11; controls were administered vehicle only. Dams were killed by cervical dislocation 4 hr after treatment and forelimb buds were isolated from both the control and treated embryos. Proteins in forelimb buds GD 11 + 4 hr were precipitated out using 40-60% ammonium sulfate and were then analyzed by 2D SDS-PAGE. Excised protein spots were identified by mass spectrometry and amino acid internal sequence analysis. Identified protein was further confirmed by Western blotting. RESULTS: Two-dimensional gel analysis indicated that 1 protein spot of 81.7 kDa/pI 7.3 was overexpressed, and the protein matched heat shock protein 70 (HSP70; accession no. P08109, SwissProt). CONCLUSIONS: The results suggest that MAA, when administered to pregnant mice, upregulates HSP70 in the forelimb buds
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