19 research outputs found

    E-Cigarette Affects the Metabolome of Primary Normal Human Bronchial Epithelial Cells

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    <div><p>E-cigarettes are widely believed to be safer than conventional cigarettes and have been even suggested as aids for smoking cessation. However, while reasonable with some regards, this judgment is not yet supported by adequate biomedical research data. Since bronchial epithelial cells are the immediate target of inhaled toxicants, we hypothesized that exposure to e-cigarettes may affect the metabolome of human bronchial epithelial cells (HBEC) and that the changes are, at least in part, induced by oxidant-driven mechanisms. Therefore, we evaluated the effect of e-cigarette liquid (ECL) on the metabolome of HBEC and examined the potency of antioxidants to protect the cells. We assessed the changes of the intracellular metabolome upon treatment with ECL in comparison of the effect of cigarette smoke condensate (CSC) with mass spectrometry and principal component analysis on air-liquid interface model of normal HBEC. Thereafter, we evaluated the capability of the novel antioxidant tetrapeptide O-methyl-l-tyrosinyl-γ-l-glutamyl-l-cysteinylglycine (UPF1) to attenuate the effect of ECL. ECL caused a significant shift in the metabolome that gradually gained its maximum by the 5<sup>th</sup> hour and receded by the 7<sup>th</sup> hour. A second alteration followed at the 13<sup>th</sup> hour. Treatment with CSC caused a significant initial shift already by the 1<sup>st</sup> hour. ECL, but not CSC, significantly increased the concentrations of arginine, histidine, and xanthine. ECL, in parallel with CSC, increased the content of adenosine diphosphate and decreased that of three lipid species from the phosphatidylcholine family. UPF1 partially counteracted the ECL-induced deviations, UPF1’s maximum effect occurred at the 5<sup>th</sup> hour. The data support our hypothesis that ECL profoundly alters the metabolome of HBEC in a manner, which is comparable and partially overlapping with the effect of CSC. Hence, our results do not support the concept of harmlessness of e-cigarettes.</p></div

    Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor-7

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    F-β(E) for 48 h. B: Increased expression of Tn was detected by Western blot analysis in cell lysates after stimulation with TGF-β(B) for 48 h. The data were quantified by densitometry and are expressed as fold increases above non-stimulated cells (mean ± SEM) (n = 12). D: Augmented expression of Tn protein was detected by flow cytometry in response to LTDcombined with TGF-β, compared with the effect of LTDalone. Difference from isotype control mean fluorescence intensity (MFI) is presented (mean ± SEM). Presence of 5 mM L-cysteine in the culture media did not affect the expression levels of Tn (D, E). *p < 0.05, **p < 0.01, and ***p < 0.001 vs. non-stimulated cells. NS – non-significant.<p><b>Copyright information:</b></p><p>Taken from "Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor"</p><p>http://respiratory-research.com/content/9/1/44</p><p>Respiratory Research 2008;9(1):44-44.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2412865.</p><p></p

    H. pylori Adheres to Erythrocytes in Capillaries and Post-Capillary Venules of Infected Humans and Rhesus Monkeys

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    <div><p>(A) Genta-stained section of human gastric biopsy. Black spiral- and comma-shaped bacteria are observed in the lumen of the stomach, adherent to gastric epithelial cells, within the mucus globule of the cells. Bacterial cells (arrow) are also present in close contact to an erythrocyte within a capillary located in the supporting connective tissue of lamina propria of the mucosa.</p><p>(B) Section of human gastric biopsy stained with toluidine blue. A capillary vessel lined by endothelial cells is visible in the lamina propria of the mucosa. It contains several erythrocytes to which H. pylori are attached. Insert: higher magnification of two H. pylori (arrows) in close approximation to erythrocytes.</p><p>(C) Section of a Rhesus monkey gastric biopsy. In situ hybridization was performed using probes specific for H. pylori 16S rRNA, demonstrating the presence of several H. pylori apparently attached to erythrocyte surfaces of a post-capillary venule located in the lamina propria of submucosa. Inserts: higher magnification of H. pylori bacterial cells (arrows) in close approximation to erythrocytes.</p><p>(D) Section of a human gastric biopsy. In situ hybridization was performed using probes specific for H. pylori 16S rRNA. This high magnification of a capillary immediately adjacent to a gastric gland (on the top-right corner of the picture) demonstrates the presence of several H. pylori bacterial cells, stained blue, apparently attached to the surfaces of erythrocytes.</p><p>Bars = 5 μm.</p></div

    Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor-5

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    BAY u9773. There was no statistical difference between the inhibitory effects of MLK and BAY u9773. Data are expressed as mean ± SEM (n = 12) *p < 0.05, **p < 0.01, and ***p < 0.001.<p><b>Copyright information:</b></p><p>Taken from "Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor"</p><p>http://respiratory-research.com/content/9/1/44</p><p>Respiratory Research 2008;9(1):44-44.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2412865.</p><p></p

    Characterization of Binding Properties of the SabA Adhesin, and Its siaHA Properties

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    <div><p>(A) A panel of 99 Swedish clinical H. pylori isolates was tested for sia-HA properties and for binding to <sup>125</sup>I-sdiLex conjugate. The numbers on the <i>x</i> axis indicate the shifts in HA titers after sialidase treatment: positive values indicate lowered sia-HA titers (i.e., sia-HA, whereas negative values indicate increased HA titers (i.e., sialic acid–independent HA). No change in HA titer is indicated by 0. The <i>y</i> axis gives the percentage of bound sLex-conjugate.</p><p>(B) SabA was affinity-adsorbed to erythrocytes from a cell-surface protein extract of strain J99. Immunostaining using SabA antibodies confirmed the presence of SabA adsorbed onto the erythrocyte surfaces as a result of binding to sialylated glycans (lane 1), whereas SabA was completely absent when erythrocytes had been depleted of sialic acid by sialidase treatment prior to the test (lane 2). Molecular weight markers (in kDa) are indicated.</p><p>(C) Binding of H. pylori strains J99 and J99<i>sabA</i> to human erythrocyte glycosphingolipids. (i) Chemical detection by anisaldehyde. (ii–iii) Autoradiograms obtained by binding of <sup>35</sup>S-labeled H. pylori strain J99 and the J99<i>sabA</i> mutant, respectively, to separated glycosphingolipids. The lanes contain non-acid glycosphingolipids of human erythrocytes, 40 μg (lane 1); gangliosides of human erythrocytes, 40 μg (lane 2); GM3 ganglioside (NeuAcα2–3Galβ4Glcβ1Cer), 4 μg (lane 3); NeuAcα2–3-neolactotetraocylceramide</p><p>(NeuAcα2–3Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 4 μg (lane 4); NeuAcα2–6-neolactotetraocylceramide</p><p>(NeuAcα2–6Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 4 μg (lane 5); G-10 ganglioside (NeuAcα2–3Galβ4GlcNAcβ6 (NeuAcα2–3Galβ4GlcNAcβ3)Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 1 μg (lane 6); G9-B ganglioside (Galα3(Fucα2)Galβ4GlcNAcβ6</p><p>(NeuAcα2–3Galβ4GlcNAcβ3)Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 1 μg (lane 7); and reference gangliotriaosylceramide (GalNAcβ4Galβ4Glcβ1Cer) of mouse feces, 4 μg (lane 8).</p></div

    Mass spectrum of primary normal human bronchial epithelial cells cultivated in air-liquid interface after exposure to e-cigarette liquid (ECL) (100 μM by nicotine) and 10 μg/mL cigarette smoke condensate (CSC) consisting of 1,822 distinct mass-to-charge signals.

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    <p>The figure represents the proportions of the spectrum that were significantly (p<i><</i>0.05) affected by addition of ECL (392 signals) or CSC (569 signals) during the first 7 h. There were 138 signals that were significantly affected by both stimuli.</p

    Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor-1

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    F-β(E) for 48 h. B: Increased expression of Tn was detected by Western blot analysis in cell lysates after stimulation with TGF-β(B) for 48 h. The data were quantified by densitometry and are expressed as fold increases above non-stimulated cells (mean ± SEM) (n = 12). D: Augmented expression of Tn protein was detected by flow cytometry in response to LTDcombined with TGF-β, compared with the effect of LTDalone. Difference from isotype control mean fluorescence intensity (MFI) is presented (mean ± SEM). Presence of 5 mM L-cysteine in the culture media did not affect the expression levels of Tn (D, E). *p < 0.05, **p < 0.01, and ***p < 0.001 vs. non-stimulated cells. NS – non-significant.<p><b>Copyright information:</b></p><p>Taken from "Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor"</p><p>http://respiratory-research.com/content/9/1/44</p><p>Respiratory Research 2008;9(1):44-44.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2412865.</p><p></p

    Different Sialyl-Dependent Binding Modes of H. pylori Identified by Use of sdiLex, sLea, and sLn Conjugates

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    <p>A total of 39 Swedish clinical isolates were investigated for detailed sialyl-dependent binding properties. Representatives of the different binding modes for <sup>125</sup>I-labeled sialylated glycans are illustrated in the diagram: (i) (in thick line) binds efficiently to all three sialylated glycans with preferential binding to sdiLex; (ii) binds to all three sialylated glycans, with better binding to sLea (“Λ-shaped” hatched line); (iii) binds preferentially to sdiLex and sLn(14) (“V-shaped” dotted line); (iv) binds preferentially to sdiLex but exhibits only modest binding for sLea and sLn(14) sialyl conjugates; and (v) binds modestly for all sialyl conjugates (<5% bound conjugate). The <i>y</i> axis gives the percentage of bound conjugate.</p

    Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor-6

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    O LTD(A) and to LTE(B) for 48 h. The data were quantified by densitometry and are expressed as fold increases above non-stimulated cells (mean ± SEM) (n = 12). C: Immunoperoxidase immunocytochemistry for Tn showing the basal expression of Tn and an increase by treatment with LTDand with LTE. Images are reproduced from a single experiment, but are representative findings of multiple staining experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. non-stimulated cells.<p><b>Copyright information:</b></p><p>Taken from "Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor"</p><p>http://respiratory-research.com/content/9/1/44</p><p>Respiratory Research 2008;9(1):44-44.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2412865.</p><p></p
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