22 research outputs found

    Limit of detection <sup>a</sup> of the fungal Luminex assay.

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    <p>Limit of detection <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0173390#t006fn001" target="_blank"><sup>a</sup></a> of the fungal Luminex assay.</p

    Proof of concept with environmental samples: Culture, microscopic determination and quantification.

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    <p>Proof of concept with environmental samples: Culture, microscopic determination and quantification.</p

    Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to <i>Salmonella enterica</i> serotype Typhimurium and serotype 1,4,[5],12:i:-

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    <div><p>Whole genome sequencing represents a promising new technology for subtyping of bacterial pathogens. Besides the technological advances which have pushed the approach forward, the last years have been marked by considerable evolution of the whole genome sequencing data analysis methods. Prior to application of the technology as a routine epidemiological typing tool, however, reliable and efficient data analysis strategies need to be identified among the wide variety of the emerged methodologies. In this work, we have compared three existing SNP-based subtyping workflows using a benchmark dataset of 32 <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Typhimurium and serovar 1,4,[<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0192504#pone.0192504.ref005" target="_blank">5</a>],12:i:- isolates including five isolates from a confirmed outbreak and three isolates obtained from the same patient at different time points. The analysis was carried out using the original (high-coverage) and a down-sampled (low-coverage) datasets and two different reference genomes. All three tested workflows, namely CSI Phylogeny-based workflow, CFSAN-based workflow and PHEnix-based workflow, were able to correctly group the confirmed outbreak isolates and isolates from the same patient with all combinations of reference genomes and datasets. However, the workflows differed strongly with respect to the SNP distances between isolates and sensitivity towards sequencing coverage, which could be linked to the specific data analysis strategies used therein. To demonstrate the effect of particular data analysis steps, several modifications of the existing workflows were also tested. This allowed us to propose data analysis schemes most suitable for routine SNP-based subtyping applied to <i>S</i>. Typhimurium and <i>S</i>. 1,4,[<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0192504#pone.0192504.ref005" target="_blank">5</a>],12:i:-. Results presented in this study illustrate the importance of using correct data analysis strategies and to define benchmark and fine-tune parameters applied within routine data analysis pipelines to obtain optimal results.</p></div