6 research outputs found

    RanBP9 overexpression does not alter synaptic protein levels in the cortex and hippocampus at 3- months of age.

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    <p><b>A:</b> Shows an immunoblotting analysis of rab3A, gap43, drebrin, chromogranin and the house keeping gene actin in brain samples from cortex and hippocampus. Brain homogenates from RanBP9 transgenic (Ran), APΔE9 double transgenic (Dbl), APΔE9/RanBP9 triple transgenic (Tpl) and age-matched wild-type (WT) control mice at 3-months of age were subjected to SDS-PAGE electrophoresis and probed with their respective antibodies. Flag specific monoclonal antibody detected flag-tagged exogenous RanBP9 in the RanBP9 single transgenic and APΔE9/RanBP99 triple transgenic mice only. Actin was used as loading control. The numbers on the left side indicate the molecular weights of each protein. <b>B:</b> Image J quantitation and normalization to actin levels showed no changes in the levels of any of the synaptic proteins at 3 months. The data are mean±SEM, n = 6 for WT and RanBP9 single transgenic, and n = 8 for APΔE9 and APΔE9/RanBP9 genotypes.</p

    Immunohistochemical evidence for the reduced synaptic proteins at 6-months of age in the CA1 region of the hippocampus.

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    <p>Hippocampal brain sections from wild-type (WT) and APΔE9/RanBP9 triple transgenic mice were stained with antibodies against rab3a, gap43, drebrin and chromogranin. A qualitative difference is clearly seen with reduced immunoreactive puncta in the triple transgenic mice compared to WT brains for all the four synaptic proteins (red). The neuronal nuclei are stained blue. Scale bar: 100 µm.</p

    RanBP9 overexpression does not alter synaptic protein levels in the cortex and hippocampus at 4- months of age.

    No full text
    <p><b>A:</b> Shows an immunoblotting analysis of rab3A, gap43, drebrin, chromogranin and the house keeping gene actin in brain samples from cortex and hippocampus. Brain homogenates from RanBP9 transgenic (Ran), APΔE9 double transgenic (Dbl), APΔE9/RanBP9 triple transgenic (Tpl) and age-matched wild-type (WT) control mice at 4-months of age were subjected to SDS-PAGE electrophoresis and probed with their respective antibodies. Flag specific monoclonal antibody detected flag-tagged exogenous RanBP9 in the RanBP9 single transgenic and APΔE9/RanBP99 triple transgenic mice only. Actin was used as loading control. The numbers on the left side indicate the molecular weights of each protein. <b>B:</b> Image J quantitation and normalization to actin levels showed no changes in the levels of any of the synaptic proteins at 4 months. The data are mean±SEM, n = 6 for WT and RanBP9 single transgenic, and n = 8 for APΔE9 and APΔE9/RanBP9 genotypes.</p

    Nondestructive Thickness Mapping of Wafer-Scale Hexagonal Boron Nitride Down to a Monolayer

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    The availability of an accurate, nondestructive method for measuring thickness and continuity of two-dimensional (2D) materials with monolayer sensitivity over large areas is of pivotal importance for the development of new applications based on these materials. While simple optical contrast methods and electrical measurements are sufficient for the case of metallic and semiconducting 2D materials, the low optical contrast and high electrical resistivity of wide band gap dielectric 2D materials such as hexagonal boron nitride (hBN) hamper their characterization. In this work, we demonstrate a nondestructive method to quantitatively map the thickness and continuity of hBN monolayers and bilayers over large areas. The proposed method is based on acquisition and subsequent fitting of ellipsometry spectra of hBN on Si/SiO<sub>2</sub> substrates. Once a proper optical model is developed, it becomes possible to identify and map the commonly observed polymer residuals from the transfer process and obtain submonolayer thickness sensitivity for the hBN film. With some assumptions on the optical functions of hBN, the thickness of an as-transferred hBN monolayer on SiO<sub>2</sub> is measured as 4.1 Å ± 0.1 Å, whereas the thickness of an air-annealed hBN monolayer on SiO<sub>2</sub> is measured as 2.5 Å ± 0.1 Å. We argue that the difference in the two measured values is due to the presence of a water layer trapped between the SiO<sub>2</sub> surface and the hBN layer in the latter case. The procedure can be fully automated to wafer scale and extended to other 2D materials transferred onto any polished substrate, as long as their optical functions are approximately known

    Fast, Noncontact, Wafer-Scale, Atomic Layer Resolved Imaging of Two-Dimensional Materials by Ellipsometric Contrast Micrography

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    Adequate characterization and quality control of atomically thin layered materials (2DM) has become a serious challenge particularly given the rapid advancements in their large area manufacturing and numerous emerging industrial applications with different substrate requirements. Here, we focus on ellipsometric contrast micrography (ECM), a fast intensity mode within spectroscopic imaging ellipsometry, and show that it can be effectively used for noncontact, large area characterization of 2DM to map coverage, layer number, defects and contamination. We demonstrate atomic layer resolved, quantitative mapping of chemical vapor deposited graphene layers on Si/SiO<sub>2</sub>-wafers, but also on rough Cu catalyst foils, highlighting that ECM is applicable to all application relevant substrates. We discuss the optimization of ECM parameters for high throughput characterization. While the lateral resolution can be less than 1 μm, we particularly explore fast scanning and demonstrate imaging of a 4″ graphene wafer in 47 min at 10 μm lateral resolution, i.e., an imaging speed of 1.7 cm<sup>2</sup>/min. Furthermore, we show ECM of monolayer hexagonal BN (h-BN) and of h-BN/graphene bilayers, highlighting that ECM is applicable to a wide range of 2D layered structures that have previously been very challenging to characterize and thereby fills an important gap in 2DM metrology

    DataSheet_1_A delta-radiomics model for preoperative prediction of invasive lung adenocarcinomas manifesting as radiological part-solid nodules.docx

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    PurposeThis study aims to explore the value of the delta-radiomics (DelRADx) model in predicting the invasiveness of lung adenocarcinoma manifesting as radiological part-solid nodules (PSNs).MethodsA total of 299 PSNs histopathologically confirmed as lung adenocarcinoma (training set, n = 209; validation set, n = 90) in our hospital were retrospectively analyzed from January 2017 to December 2021. All patients underwent diagnostic noncontrast-enhanced CT (NCECT) and contrast-enhanced CT (CECT) before surgery. After image preprocessing and ROI segmentation, 740 radiomic features were extracted from NCECT and CECT, respectively, resulting in 740 DelRADx. A DelRADx model was constructed using the least absolute shrinkage and selection operator logistic (LASSO-logistic) algorithm based on the training cohort. The conventional radiomics model based on NCECT was also constructed following the same process for comparison purposes. The prediction performance was assessed using area under the ROC curve (AUC). To provide an easy-to-use tool, a radiomics-based integrated nomogram was constructed and evaluated by integrated discrimination increment (IDI), calibration curves, decision curve analysis (DCA), and clinical impact plot.ResultsThe DelRADx signature, which consisted of nine robust selected features, showed significant differences between the AIS/MIA group and IAC group (p ConclusionThe DelRADx method has the potential to assist doctors in predicting the invasiveness for patients with PSNs. The integrated nomogram incorporating the DelRADx signature with the radiographic features could facilitate the performance and serve as an alternative way for determining management.</p
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