13 research outputs found

    Multifaceted Role of Heme during Severe Plasmodium falciparum Infections in India

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    Several immunomodulatory factors are involved in malaria pathogenesis. Among them, heme has been shown to play a role in the pathophysiology of severe malaria in rodents, but its role in human severe malaria remains unclear. Circulating levels of total heme and its main scavenger, hemopexin, along with cytokine/chemokine levels and biological parameters, including hemoglobin and creatinine levels, as well as transaminase activities, were measured in the plasma of 237 Plasmodium falciparum-infected patients living in the state of Odisha, India, where malaria is endemic. All patients were categorized into well-defined groups of mild malaria, cerebral malaria (CM), or severe noncerebral malaria, which included acute renal failure (ARF) and hepatopathy. Our results show a significant increase in total plasma heme levels with malaria severity, especially for CM and malarial ARF. Spearman rank correlation and canonical correlation analyses have shown a correlation between total heme, hemopexin, interleukin-10, tumor necrosis factor alpha, gamma interferon-induced protein 10 (IP-10), and monocyte chemotactic protein 1 (MCP-1) levels. In addition, canonical correlations revealed that heme, along with IP-10, was associated with the CM pathophysiology, whereas both IP-10 and MCP-1 together with heme discriminated ARF. Altogether, our data indicate that heme, in association with cytokines and chemokines, is involved in the pathophysiology of both CM and ARF but through different mechanisms.Indo-French Centre for the Promotion of Advanced Research, Associated International Laboratory Systems (LIA; CNRS), Immunology and Genetics of Infectious Diseases (SIGID), Department of Biotechnology from the Ministry of Science and Technology of India (DBT), Tata Institute of Fundamental Research (TIFR) (intramural funds), Université Lille (doctoral contract), IFCPAR (Raman-Charpak award), College Doctoral Lille Nord de France (AAP n10 award), Fondation des Treille, Conseil Régional du Nord-Pas de Calais

    Forest plots of CR1 intron 27 polymorphism in association to severe malaria.

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    <p>Meta-analysis was performed including previous reports and current study by comprehensive meta-analysis software. Random or fixed model of meta-analysis was employed for calculation of the combined effect of all studies. Forest plots evaluating resistance/risk factor of different models to severe malaria are shown.</p

    Haplotype analysis of CR1polymorphisms in <i>P. falciparum</i> malaria patients.

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    <p>NOTE: Data are shown in percentage. UM: uncomplicated malaria; CM: cerebral malaria; MOD: multi-organ dysfunction; NCSM: non-cerebral severe malaria; OR: odds ratio; CI: confidence interval.</p

    Characteristics of all studies included in meta-analysis.

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    <p>NOTE: Data are no. (%) of participants unless otherwise specified. SM; severe malaria, MM; Mild malaria, SMA; severe malaria anemia, PNG; Papua New Guinea, RFLP; restriction fragment length polymorphisms, NA; not associated, ND; not done.</p

    Genotype and allele of CR1 (exon 22 and intron 27) polymorphisms and blood groups distribution in <i>P. falciparum</i> malaria.

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    <p>NOTE: Data are no. (%) of participants unless otherwise specified. UM: uncomplicated malaria; CM: cerebral malaria; MOD: multi-organ dysfunction; NCSM: non-cerebral severe malaria; OR: odds ratio; CI: confidence interval.</p

    Forest plots of CR1 exon 22 polymorphism in association to severe malaria.

    No full text
    <p>Meta-analysis was performed including previous reports and current study by comprehensive meta-analysis software. Random or fixed model of meta-analysis was employed for calculation of the combined effect of all studies. Forest plots evaluating resistance/risk factor of different models to severe malaria are shown.</p
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