85 research outputs found

    The fitness landscape of the African salmonella typhimurium ST313 strain D23580 reveals unique properties of the pBT1 plasmid

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    We have used a transposon insertion sequencing (TIS) approach to establish the fitness landscape of the African Salmonella enterica serovar Typhimurium ST313 strain D23580, to complement our previous comparative genomic and functional transcriptomic studies. We used a genome-wide transposon library with insertions every 10 nucleotides to identify genes required for survival and growth in vitro and during infection of murine macrophages. The analysis revealed genomic regions important for fitness under two in vitro growth conditions. Overall, 724 coding genes were required for optimal growth in LB medium, and 851 coding genes were required for growth in SPI-2-inducing minimal medium. These findings were consistent with the essentiality analyses of other S. Typhimurium ST19 and S. Typhi strains. The global mutagenesis approach also identified 60 sRNAs and 413 intergenic regions required for growth in at least one in vitro growth condition. By infecting murine macrophages with the transposon library, we identified 68 genes that were required for intra-macrophage replication but did not impact fitness in vitro. None of these genes were unique to S. Typhimurium D23580, consistent with a high conservation of gene function between S. Typhimurium ST313 and ST19 and suggesting that novel virulence factors are not involved in the interaction of strain D23580 with murine macrophages. We discovered that transposon insertions rarely occurred in many pBT1 plasmid-encoded genes (36), compared with genes carried by the pSLT-BT virulence plasmid and other bacterial plasmids. The key essential protein encoded by pBT1 is a cysteinyl-tRNA synthetase, and our enzymological analysis revealed that the plasmid-encoded CysRSpBT1 had a lower ability to charge tRNA than the chromosomally-encoded CysRSchr enzyme. The presence of aminoacyl-tRNA synthetases in plasmids from a range of Gram-negative and Gram-positive bacteria suggests that plasmid-encoded essential genes are more common than had been appreciated

    A measurement of lifetime differences in the neutral D-meson system

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    Using a high statistics sample of photoproduced charm particles from the FOCUS experiment at Fermilab, we compare the lifetimes of neutral D mesons decaying via D0 to K- pi+ and K- K+ to measure the lifetime differences between CP even and CP odd final states. These measurements bear on the phenomenology of D0 - D0bar mixing. If the D0 to K-pi+ is an equal mixture of CP even and CP odd eigenstates, we measure yCP = 0.0342 \pm 0.0139 \pm 0.0074.Comment: 15 pages, 5 figure

    Синтез и исследование кристаллической структуры гидратированного комплекса додекавольфрамосиликата с протонированным 1,2-фенилендиамином состава (C<sub>6</sub>H<sub>9</sub>N<sub>2</sub>)<sub>3</sub>(Н<sub>3</sub>О)SiW<sub>12</sub>O<sub>40</sub> · 8H<sub>2</sub>O

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    The hydrated dodecatungstatosilicate complex with protonated ortho-phenylenediamine (C6H9N2)(3)(De(3)O)SiW12O40 center dot 8H(2)O has been synthesized and studied by X-ray diffraction. Crystals are triclinic, space group P-1, a = 13.6817(5) angstrom, b =1 4.5915(5) angstrom , c = 15.8844(5) angstrom, alpha = 98.891(3)degrees, beta = 101.836(3)degrees, gamma = 114.156(2)degrees, V = 2729.05(16) angstrom(3), rho(calcd) = 4.100 g/cm(3), Z = 2.Синтезирован и исследован методом РСА гидратированный комплекс додекавольфрамосиликата с протонированным орто-фенилендиамином состава (C6H9N2)3(Н3О)SiW12O40 ⋅ 8H2O. Кристаллы триклинные, пр. гр. Р-1: a = 13.6817(5), b = 1 4.5915(5), с = 15.8844(5) Å, α = 98.891(3)°, β = 101.836(3)°, γ = 114.156(2)°, V = 2729.05(16) Å3, ρвыч = 4.100 г/см3, Z = 2

    Hydrated Dodecatungstatosilicate Complex with Protonated 1,2-Phenylenediamine (C6H9N2)3(H3O)SiW12O40 · 8H2O: Synthesis and Crystal Structure

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    The hydrated dodecatungstatosilicate complex with protonated ortho-phenylenediamine (C6H9N2)3(Н3O)SiW12O40 · 8H2O has been synthesized and studied by X-ray diffraction. Crystals are triclinic, space group Р-1, a = 13.6817(5) Å, b =1 4.5915(5) Å, с = 15.8844(5) Å, α = 98.891(3)°, β = 101.836(3)°, γ = 114.156(2)°, V = 2729.05(16) Å3, ρcalcd = 4.100 g/cm3, Z = 2. © 2018, Pleiades Publishing, Ltd

    Синтез и исследование кристаллической структуры гидратированного комплекса додекавольфрамосиликата с протонированным 1,2-фенилендиамином состава (C<sub>6</sub>H<sub>9</sub>N<sub>2</sub>)<sub>3</sub>(Н<sub>3</sub>О)SiW<sub>12</sub>O<sub>40</sub> · 8H<sub>2</sub>O

    No full text
    The hydrated dodecatungstatosilicate complex with protonated ortho-phenylenediamine (C6H9N2)(3)(De(3)O)SiW12O40 center dot 8H(2)O has been synthesized and studied by X-ray diffraction. Crystals are triclinic, space group P-1, a = 13.6817(5) angstrom, b =1 4.5915(5) angstrom , c = 15.8844(5) angstrom, alpha = 98.891(3)degrees, beta = 101.836(3)degrees, gamma = 114.156(2)degrees, V = 2729.05(16) angstrom(3), rho(calcd) = 4.100 g/cm(3), Z = 2.Синтезирован и исследован методом РСА гидратированный комплекс додекавольфрамосиликата с протонированным орто-фенилендиамином состава (C6H9N2)3(Н3О)SiW12O40 ⋅ 8H2O. Кристаллы триклинные, пр. гр. Р-1: a = 13.6817(5), b = 1 4.5915(5), с = 15.8844(5) Å, α = 98.891(3)°, β = 101.836(3)°, γ = 114.156(2)°, V = 2729.05(16) Å3, ρвыч = 4.100 г/см3, Z = 2

    Hydrated Dodecatungstatosilicate Complex with Protonated 1,2-Phenylenediamine (C6H9N2)3(H3O)SiW12O40 · 8H2O: Synthesis and Crystal Structure

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    The hydrated dodecatungstatosilicate complex with protonated ortho-phenylenediamine (C6H9N2)3(Н3O)SiW12O40 · 8H2O has been synthesized and studied by X-ray diffraction. Crystals are triclinic, space group Р-1, a = 13.6817(5) Å, b =1 4.5915(5) Å, с = 15.8844(5) Å, α = 98.891(3)°, β = 101.836(3)°, γ = 114.156(2)°, V = 2729.05(16) Å3, ρcalcd = 4.100 g/cm3, Z = 2. © 2018, Pleiades Publishing, Ltd

    Clinical Utility of Echocardiography

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