48 research outputs found

    Characteristics of mutation-confirmed PHP1A cohort.

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    Characteristics of mutation-confirmed PHP1A cohort.</p

    Alterations of ocular surface parameters in patients with obstructive sleep apnea syndrome

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    PurposeThis study aimed to evaluate changes in ocular surface parameters among obstructive sleep apnea syndrome (OSAS) patients.Methods44 healthy volunteers (88 eyes) and 27 OSAS patients (54 eyes) were recruited in our cross-sectional study. 14 patients were classified as mild&amp;moderate OSAS, and 13 patients were classified as severe OSAS. For evaluating the ocular surface, the following tests were conducted: the height of tear meniscus (TMH), first non-invasive tear break-up time (FNITBUT), mean non-invasive tear break-up time (MNITBUT), the score of Meibomian gland dropout area (Meiboscore), the tear test of anesthesia-free Schirmer I (SIT), corneal fluorescein staining (CFS), partial blinks rate (PBR), the lipid layer thickness (LLT), ocular surface disease index (OSDI). The results obtained from the study were analyzed and compared among the groups.ResultsFNITBUT, MNITBUT, and TMH were lower. OSDI, CFS, Meiboscore and PBR were higher in the OSAS group than those in the control group. The mild&amp;moderate as well as the severe OSAS subgroups had statistically significantly lower TMH, and higher OSDI and PBR than the control group. Meanwhile, we found there were no significant differences between two OSAS subgroups. CFS was higher in the severe OSAS group than the mild&amp;moderate OSAS group. Significantly lower FNITBUT, MNITBUT and higher Meiboscore were observed in the severe OSAS subgroup than in the control group, and MNITBUT was higher in severe OSAS objects than in the mild&amp;moderate OSAS objects. LLT and SIT did not exhibit significant differences among control and OSAS subgroups. FNITBUT and MNITBUT showed significantly negative correlations with BMI, while Meiboscore showed a significant positive correlation with AHI.ConclusionPatients with OSAS have a tendence of dry eyes, whereas control subjects do not. This indicates us that the OSAS patients should pay more attention to ocular surface care

    Accelerated reduction in spheno-occipital synchondrosis length within E1+/- mice is associated with a reduction in the number of proliferating chondrocytes.

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    (A-F) Representative images of the SOS (yellow line) in (A-C) 1-week-old mice and (D-F) 2-week-old mice. In Fig 6 (A, D) are WT, (B, E) are Gnas E1+/-p, and (C, F) are Gnas E1+/-m mice stained for Alkaline phosphatase (red), EdU (green), and DAPI (blue). (G) Quantification of the percentage of EdU+ chondrocytes to the total number of chondrocytes within the resting and proliferative zone at 1 week of age demonstrates that both Gnas E1+/-p and Gnas E1+/-m mice display a significant reduction in proliferating chondrocytes compared to WT. No significant differences were observed at 2 weeks of age. (H) Quantification of the resting and proliferative zone lengths (defined as ALP negative zone of synchondrosis) (blue line) were significantly reduced in both Gnas E1+/-p and Gnas E1+/-m mice when compared to WT at 1 week of age. No significant differences were observed at 2 weeks of age. (I) Quantification of the hypertrophic zone length (defined as ALP+ zone of synchondrosis) (orange lines) demonstrated no significant differences between WT and Gnas E1+/- mice at 1 or 2 weeks of age. Sample size per genotype per experiment is listed on each graph. All statistical tests were completed using a two-way ANOVA with post-hoc Tukey test for multiple comparisons, and p-values are displayed for each comparison.</p

    Correlation of lifestyle behaviors during pregnancy with postpartum depression status of puerpera in the rural areas of South China

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    BackgroundPostpartum depression (PPD) is among the most common postpartum complications. Its prevalence is associated with strong regional variability. Women in rural areas of China have a high risk of PPD. The aim of this study was to investigate the PPD status of women in rural South China and explore the effects of modifiable lifestyle behaviors during pregnancy on their PPD status, thereby providing a scientific basis for the prevention and intervention of PPD in rural China.MethodsA cohort study was conducted on 261 women from four maternal health institutions situated in rural areas of Guangdong Province and the Guangxi Zhuang Autonomous Region from October 2021 to December 2022. The questionnaires were administered to these women to obtain data about sociodemographic characteristics, health literacy, physical activity during pregnancy, and sleep and dietary status during pregnancy, as well as depression status on the 42nd day after delivery. The lifestyle behaviors during pregnancy and the PPD status of the study population were analyzed. Multiple linear regression models were used to determine the correlation between lifestyle behaviors and PPD status. Path analysis was performed to explore the interaction between various lifestyle behaviors.ResultsA total of 14.6% of women had a PPD status. Women who continued to work during pregnancy had an Edinburgh Postpartum Depression Scale (EPDS) score of 1.386 points higher than that of women who did not (В = 1.386, β = 0.141, p = 0.029). For every 1-point increase in the infant feeding-related knowledge score and pregnancy diet diversity score, the EPDS score decreased by 0.188 and 0.484 points, respectively, and for every 1-point increase in the Pittsburgh sleep quality index score, the EPDS score increased by 0.288 points. Age was related to infant feeding-related knowledge (indirect path coefficient = 0.023). During pregnancy, sedentary time was correlated with sleep quality (indirect path coefficient = 0.031) and employment status (indirect path coefficient = 0.043).ConclusionEmployment status, infant feeding-related knowledge, sleep quality, and diet diversity during pregnancy directly influenced the PPD status, while age and sedentary time during pregnancy indirectly influenced the PPD status. Promoting healthy lifestyle behaviors, including reducing sedentary time, improving sleep quality, and increasing dietary diversity, may be effective in reducing PPD occurrence

    <i>Gnas</i> E1+/-m mice display cranial hyperostosis and enhanced calvarial bone formation <i>in vivo</i>.

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    (A) Representative 3D reconstruction of the cranial vault of 12-week WT, Gnas E1+/-p, and Gnas E1+/-m mice demonstrating hyperostosis within Gnas E1+/-m mice. (B) Representative calcein and alizarin complexone double labeling on the calvaria of 12-week WT, Gnas E1+/-p, and Gnas E1+/-m mice. (C-E) Quantification of (C) Bone formation rate (BFR); (D) mineralizing surface to bone surface (MS/BS); and (E) Mineral apposition rate (MAR) within the calvaria demonstrates Gnas E1+/-m mice display enhanced bone formation when compared to both WT and Gnas E1+/-p mice. No significant differences were observed between WT and Gnas E1+/-p mice. Sample size per genotype per experiment is listed on each graph. All statistical tests were completed using ANOVA with post-hoc Tukey test for multiple comparisons, and p-values are displayed for each comparison.</p

    Prevalence of Chiari malformation type 1 is increased in pseudohypoparathyroidism type 1A and associated with aberrant bone development.

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    BackgroundAlbright hereditary osteodystrophy (AHO) is caused by heterozygous inactivating mutations in GNAS. Patients with maternally-inherited mutations develop pseudohypoparathyroidism type 1A (PHP1A) with multi-hormone resistance and aberrant craniofacial and skeletal development among other abnormalities. Chiari malformation type 1 (CM1), a condition in which brain tissue extends into the spinal canal when the skull is too small, has been reported in isolated cases of PHP1A. It has been hypothesized to be associated with growth hormone (GH) deficiency. Given the adverse clinical sequelae that can occur if CM1 goes unrecognized, we investigated the previously undetermined prevalence of CM1, as well as any potential correlations with GH status, given the known increased prevalence of GH deficiency in PHP1A. We also investigated these metrics for low lying cerebellar tonsils (LLCT), defined as tonsillar descent less than 5 mm below the foramen magnum. In addition, we investigated possible correlations of CM1/LLCT with advanced hand/wrist bone ages and craniofacial abnormalities known to occur in PHP1A to determine whether premature chondrocyte differentiation and/or aberrant craniofacial development could be potential etiologies of CM1/LLCT through both human studies and investigations of our AHO mouse model.MethodsWe examined patients with PHP1A in our clinic and noticed CM1 more frequently than expected. Therefore, we set out to determine the true prevalence of CM1 and LLCT in a cohort of 54 mutation-confirmed PHP1A participants who had clinically-indicated brain imaging. We examined potential correlations with GH status, clinical features, biological sex, genotype, and hand/wrist bone age determinations. In addition, we investigated the craniofacial development in our mouse model of AHO (Gnas E1+/-m) by histologic analyses, dynamic histomorphometry, and micro-computerized tomographic imaging (MCT) in order to determine potential etiologies of CM1/LLCT in PHP1A.ResultsIn our cohort of PHP1A, the prevalence of CM1 is 10.8%, which is at least 10-fold higher than in the general population. If LLCT is included, the prevalence increases to 21.7%. We found no correlation with GH status, biological sex, genotype, or hand/wrist bone age. Through investigations of our Gnas E1+/-m mice, the correlate to PHP1A, we identified a smaller cranial vault and increased cranial dome angle with evidence of hyperostosis due to increased osteogenesis. We also demonstrated that there was premature closure of the spheno-occipital synchondrosis (SOS), a cartilaginous structure essential to the development of the cranial base. These findings lead to craniofacial abnormalities and could contribute to CM1 and LLCT development in PHP1A.ConclusionThe prevalence of CM1 is at least 10-fold higher in PHP1A compared to the general population and 20-fold higher when including LLCT. This is independent of the GH deficiency that is found in approximately two-thirds of patients with PHP1A. In light of potential serious consequences of CM1, clinicians should have a low threshold for brain imaging. Investigations of our AHO mouse model revealed aberrant cranial formation including a smaller cranium, increased cranial dome angle, hyperostosis, and premature SOS closure rates, providing a potential etiology for the increased prevalence of CM1 and LLCT in PHP1A

    Dissolution of antibiotics mycelium in ionic liquids: Performance and mechanism

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    Antibiotics mycelium, byproduct of pharmaceutical industry, contains high percentage of proteins, polysaccharides and lipids, while, the low solubility in traditional solvents limits its utilization. The dissolution process of penicillin mycelium was investigated using ionic liquids (ILs) as solvent. Quantitative correlation of solubility and ILs structure and dissolution mechanism were determined. About 91.45% of penicillin mycelium was dissolved in 1-butyl-3-methylimidazolium acetate ([Bmim]Ac) under the condition of 120.0 degrees C and [Bmim]Ac/mycelium (m/m) ratio of 3.90:1. Synergistic effect of ILs and DMSO was confirmed with the DMSO/Bmim]Ac (v/m) ratio in the range of 0.0-1.0. At 25.0 degrees C, the dissolution of penicillin mycelium increased from 69.74% to 94.50%, with the ratio of DMSO to [Bmim]Ac (v/m) as 1:1. The room temperature dissolution of mycelium provides a novel and energy-saving process for its high-valued utilization. The NMR and FT-IR spectra showed that hydrogen bonds are the dominant driving force for the dissolution in ILs. Quantitative study on the effects of anions and cations of ILs on dissolution using Kamlet-Taft model showed that there was a linearly positive correlation between solubility of penicillin mycelium and beta parameter of the ILs. The solubility of mycelium increased with increasing hydrogen bond accepting ability of anions and donating ability of cations. (C) 2017 The Chemical Industry and Engineering Society of China, and Chemical Industry Press. All rights reserved

    Dissolution of antibiotics mycelium in ionic liquids: Performance and mechanism

    No full text
    Antibiotics mycelium, byproduct of pharmaceutical industry, contains high percentage of proteins, polysaccharides and lipids, while, the low solubility in traditional solvents limits its utilization. The dissolution process of penicillin mycelium was investigated using ionic liquids (ILs) as solvent. Quantitative correlation of solubility and ILs structure and dissolution mechanism were determined. About 91.45% of penicillin mycelium was dissolved in 1-butyl-3-methylimidazolium acetate (BmimAc) under the condition of 120.0 °C and BmimAc °C, the dissolution of penicillin mycelium increased from 69.74% to 94.50%, with the ratio of DMSO to BmimAc (v β parameter of the ILs. The solubility of mycelium increased with increasing hydrogen bond accepting ability of anions and donating ability of cations

    Children and Adults Prefer the Egocentric Representation to the Allocentric Representation

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    We studied the strategy preference of using the egocentric or the allocentric representation in individuals who have acquired the ability to use both representations. Fifty-seven children aged 5–7 years and 53 adults retrieved toys hidden in one of four identical containers in a square room. We varied the type of spatial representation available in four conditions: (1) only self-motion information (egocentric representation); (2) only external landmark cues (allocentric representation); (3) both self-motion and landmark cues (dual representation); (4) self-motion and landmark cues in conflict (conflict trial). We found that, compared with the allocentric representation, the egocentric representation approached maturity earlier in development and was exploited better in early years. More importantly, in the conflict trials, while both children and adults relied more on egocentric representation, still a small portion of participants chose allocentric representation, especially in the adult group. These results provided evidence that egocentric representation is generally preferred more in both young children and adults

    gasassistedmagneticseparationforthepurificationofproteinsinbatchsystems

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    In this paper, gas-assisted magnetic separation (GAMS), a technique that combines magnetic separation with flotation, was investigated for the potential large-scale separation of proteins. The GAMS process includes adsorption of target proteins and magnetic separation to recover protein-loaded magnetic particles from the dilute biosuspension with the assistance of bubbles. Microsized ethylenediamine-functionalized poly(glycidyl methacrylate) superparamagnetic microspheres (MPMs) and bovine serum albumin (BSA) were used as a model system. The feasibility of GAMS for capturing BSA-loaded MPMs from an appropriate medium was shown. High recovery of BSA-loaded MPMs was obtained by simple adjustment of the initial solution pH without extra detergents and antifoaming agents. The GAMS conditions were consistent with the adsorption conditions, and no proteins were desorbed from the MPMs during this process. Under the optimal conditions, the separation rate and recovery percentage reached 410 mL/min and 98% in 0.61 min, respectively. Conformational changes of BSA during the GAMS process were investigated by fluorescence spectroscopy and circular dichroism spectrometry. (C) 2015 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved
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