14 research outputs found

    Effect of types of auxins on root induction in <i>P</i>. <i>eriocarpum</i>.

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    <p>(A)Number of roots produced per regenerated shoot. (B)Root length. (C) Basal callus formation per explant.</p

    Assessment of genetic fidelity of <i>in vitro</i> raised plants of <i>Pittosporum eriocarpum</i>.

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    <p>(A) SCoT fragments obtained with primer SCoT 35. (B) ISSR fragments obtained from primer number 860. (C)RAPD fragments obtained with primer OPL10. L- 1Kb ladder; MP- mother plant (Control); A1-A9- <i>in vitro</i> raised plants.</p

    Effect of various concentrations of BA and IBA on shoot induction from nodal explants of <i>P</i>. <i>eriocarpum</i>.

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    <p>Effect of various concentrations of BA and IBA on shoot induction from nodal explants of <i>P</i>. <i>eriocarpum</i>.</p

    Amplification products generated with SCoT, ISSR and RAPD markers among mother plant and <i>in vitro</i> raised plants.

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    <p>Amplification products generated with SCoT, ISSR and RAPD markers among mother plant and <i>in vitro</i> raised plants.</p

    Dendrogram illustrating the relatedness of serine protease gene sequence of <i>M. persicae</i> (<i>MySP</i>) with those of different Indian pollinators.

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    <p>Phylogenetic relationship of <i>MySP</i> and its counterpart from Indian pollinators was conducted using MEGA 4.0.2 which is shown along with branch lengths. DNA sequences were aligned using CLUSTAL W and a tree was constructed by neighbour-joining program from a similarity matrix of pairwise comparisons. The Nucleotide/ p-distance option was selected in substitution model section. Bootstrap values (*) were assessed with 1000 replicates and are shown at the dendrogram nodes. The scale bar represents sequence divergence.</p

    Effect of one hour incubation in IBA on number of shoots, leaves and callus diameter per explants.

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    <p>Effect of one hour incubation in IBA on number of shoots, leaves and callus diameter per explants.</p

    <i>Pittosporum eriocarpum</i>.

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    <p>(A) Nodal explant. (B) Shoot induction in MS medium supplemented with BA (mg/l). (C) Root induction in MS medium supplemented with IBA (mg/l). (D) <i>In vitro</i> grown plantlets. (E) <i>In vitro</i> grown plantlets in soil rite. (F) <i>In vitro</i> raised plantlets in natural conditions.</p

    Expression analysis of <i>MySP</i> during nymph developmental stages of <i>M. persicae</i>.

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    <p>(A) Expression profiles of <i>MySP</i> at different developmental stages of <i>M. persicae</i> ranging from early (1–2 day-old new-born nymph), median (3–5 day-old nymph) to late (6–7 day-old apterous adult) was determined by semi-quantitative RT-PCR. cDNA, prepared from 5 µg of total RNA in each sample, was PCR amplified with <i>MySP</i> specific primers (lanes 2–4; upper panel) and 18SrRNA (lanes 2–4; lower panel). The latter was used as endogenous control showing similar RNA concentrations in aphid samples collected at different developmental stages. (B) Integrated density values (IDV) of the PCR products of <i>MySP</i> in the nymphs collected at different development stages as described in (A).</p
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