15 research outputs found

    Preparations of longitudinal smooth muscle isolated from normal (A) or DNBS-treated rats (colitis) (B).

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    <p>Effects of increasing concentrations of A804598 (0.001ÔÇô10 ┬ÁM) on contractions evoked by sES (0.5 ms, 10 Hz, 30 mA, 10 s) in preparations maintained in standard Krebs solution. Each column represents the mean┬▒SEM obtained from 6 experiments. *P<0.05, versus control (CON).</p

    Double-staining immunohistochemistry showing the distribution of P2X7 receptors (green) and the neuronal marker HuC/D (red) in the myenteric plexus of colonic cryosections from control (A; normal) or DNBS-treated (B; colitis) rats.

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    <p>Nuclei were stained with TOTO-3. Scale bar: 21 ┬Ám. Enlarged view of HuC/D<sup>+</sup> and P2X7<sup>+</sup> cells in the myenteric ganglia of normal and colitis rats from boxed area in overlay (scale barÔÇŐ=ÔÇŐ10 ┬Ám). LM, longitudinal muscle; CM, circular muscle; MG, myenteric ganglia. Isotype fluorescent image was obtained by labeling with streptavidin conjugated with Alexa Fluor 555 in presence of normal mouse antiserum instead of the primary antibody.</p

    Double immunostaining showing the expression of P2X7 receptors (green) and the glial marker GFAP (red) in myenteric plexus of colonic cryosections from control (A; normal) and DNBS-treated (B; colitis) rats.

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    <p>Nuclei were stained with TOTO-3. Scale bar: 21 ┬Ám. Enlarged view of GFAP<sup>+</sup> and P2X7<sup>+</sup> cells in the myenteric ganglia of normal and colitis rats from boxed area in overlay (scale barÔÇŐ=ÔÇŐ10 ┬Ám). LM, longitudinal muscle; CM, circular muscle; MG, myenteric ganglia. Isotype fluorescent image was obtained by labeling with Alexa Fluor 555 conjugated secondary antibody in presence of normal mouse antiserum instead of the primary antibody.</p

    Apigenin decrease eosinophil infiltration in colonic tissues from HFD-mice.

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    <p>Representative microscopic pictures of haematoxylin/eosin-stained colonic sections from mice fed with SD, SD plus treatment with apigenin (10 mg/Kg/day), HFD or HFD plus treatment with apigenin (10 mg/Kg/day). The magnification within the boxed area shows the presence of eosinophils (green arrows) and neutrophils (yellow arrows). Scale bars = 50 ╬╝m; 20 ╬╝m (inset). The column graphs display the mean value of eosinophil density per square millimeter of <i>tunica mucosa/submucosa</i> areas (cell/mm<sup>2</sup>) ┬▒ SEM, n = 5. <sup>a</sup>P<0.05 significant difference <i>vs</i> SD; *P<0.05 significant difference <i>vs</i> HFD.</p
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