123 research outputs found

    <i>C. elegans</i> operons contain a higher proportion of genes that express multiple transcript isoforms.

    No full text
    <p>(A) <i>C. elegans</i> operonic genes express more transcript isoforms per gene than non-operonic genes do. (B) <i>C. elegans</i> operons contain a higher proportion of genes that express multiple transcript isoforms than non-operonic genes do. (C) Alternatively spliced <i>C. elegans</i> operonic genes and non-operonic genes have a similar number of transcript isoforms per gene. Z-test was performed (Figure 1A and 1C) to evaluate the significance of difference between the means of transcript numbers. Error bars represent standard deviations.</p

    Depletion of wildtype <i>csnk-1</i> transcripts in L4 <i>csnk-1(lf)</i> mutants.

    No full text
    (A) Positions and sequences of PCR primers for detecting all or wildtype-only csnk-1 transcripts. Partial wildtype and mutant csnk-1 sequences are aligned to show the specificity of the primers for wildtype-only transcripts. (B) Relative total csnk-1 transcript levels. (C, D) Relative wildtype-only csnk-1 transcript levels in wildtype, csnk-1(mac494lf) or csnk-1(mac495lf) animals. tba-1 was the loading control. Statistics: two-tailed unpaired Student’s t-test. *: p p p (TIFF)</p

    Geochemical Responses to Anthropogenic and Natural Influences in Ebinur Lake Sediments of Arid Northwest China

    No full text
    <div><p>Geochemical concentrations were extracted for a short sediment core from Ebinur Lake, located in arid northwest China, and mathematical methods were used to demonstrate the complex pattern of the geochemical anomalies resulting from the temporal changes in natural and anthropogenic forces on the lake sediments. The first element assemblage (C1) (aluminum, potassium, iron, magnesium, beryllium, etc.) was predominantly terrigenous; among the assemblage, total phosphorus and titanium were generally consistent with aluminum except with regards to their surface sequences, which inferred the differences of source regions for terrigenous detrital material led to this change around ca. 2000AD. The second assemblage (C2) (calcium and strontium) was found to have a negative relationship with aluminum through a cluster analysis. The third assemblage (C3) included sodium and magnesium, which were influenced by the underwater lake environment and deposited in the Ebinur depression. The concentration ratio of C1/(C1+C2) was used as an indicator for denudation amount of detrital materials, which was supported by the values of magnetic susceptibility. The enrichment factors for heavy metals suggested that the influence of human activities on heavy-metal enrichment in Ebinur Lake region was not severe over the past century. Prior to the 1960s, geochemical indicators suggested a stable lacustrine environment with higher water levels. Beginning in the 1960s, high agricultural water demand resulted in rapid declines in lake water level, with subsequent increases of lake water salinity, as evidenced by enhanced sodium concentration in lake core sediments. During this period, anthropogenic activity also enhanced the intensity of weathering and the denudation of the Ebinur watershed.</p></div

    <i>csnk-1(lf)</i> and <i>skn-1(gf)</i> mutations exhibit synergy on the Sisi phenotype in 50 mM NaI.

    No full text
    csnk-1(lf) and skn-1(gf) mutations exhibit synergy on the Sisi phenotype in 50 mM NaI.</p

    Effects of <i>csnk-1</i> transgenes on the Sisi phenotype of <i>csnk-1(lf)</i> mutants in 5 mM NaI.

    No full text
    Effects of csnk-1 transgenes on the Sisi phenotype of csnk-1(lf) mutants in 5 mM NaI.</p

    Effects of <i>csnk-1</i> mutations or variable feeding RNAis on the Sisi phenotype.

    No full text
    Mammalian orthologs or homologs are indicated in the parentheses. (TIFF)</p

    Genomic target sequences for generating <i>tsp-15</i> knockin and <i>csnk-1</i> knockout strains using the CRISPR/Cas9 method.

    No full text
    ND: not determined. For tsp-15 repair template, the letter in red was for introducing the missense mutation and letters in blue were for introducing silent mutations. (TIFF)</p

    Representative morphologies of double homozygous mutants between <i>csnk-1(lf)</i> and <i>bli-3(lf)</i> or <i>doxa-1(lf)</i>.

    No full text
    bli-3(lf) csnk-1(lf) double homozygous mutants were derived from bli-3(lf) csnk-1(lf)/hT2 heterozygous mutants. csnk-1(lf); doxa-1(lf) double homozygous mutants were derived from csnk-1(lf)/hT2; doxa-1(lf)/hT2 heterozygous mutants. Arrows point to typical blisters. All images are of the same scale. (TIFF)</p

    CSNK-1::mCherry does not colocalize with GFP and DOXA-1::GFP does not colocalize with mCherry.

    No full text
    (A, B, C) A transgenic L3 larvae co-expressing GFP and CSNK-1::mCherry in epithelial cells. (D, E, F) A transgenic 3-fold embryo co-expressing DOXA-1::GFP and mCherry in epithelial cells. For unclear reason, DOXA-1::GFP was strongly expressed in embryos but was not visible at larval stages in these transgenic lines. We therefore observed whether DOXA-1::GFP colocalizes with mCherry in embryos. (TIFF)</p

    PCR primers for generating the listed DNA fragments.

    No full text
    PCR primers for generating the listed DNA fragments.</p
    corecore