6 research outputs found

    One-Pot Multi-Tracer Synthesis of Novel <sup>18</sup>F‑Labeled PET Imaging Agents

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    <sup>18</sup>F labeled phosphonium salts are increasingly important molecular probes for targeting the mitochondrial membrane potential depletion during apoptosis and for detecting myocardial perfusion deficit. Here, we introduce three new tracers, [<sup>18</sup>F]­MitoPhos_04, [<sup>18</sup>F]­MitoPhos_05, and [<sup>18</sup>F]­MitoPhos_07, that have the potential to act as mitochondrial imaging agents. Moreover, they have the added advantage of being synthesized in the same reaction vial from one radiolabeled synthon, demonstrating a new approach to synthesizing multiple tracers in one-pot, which is a highly useful means for increasing the throughput of radiotracer development. The radiosynthesis of the tracers was carried out on a fully automated system via a facile two-step reaction. Utilizing the radiolabeling of an ethyl azide, a copper-mediated 1,3-cycloaddition reaction and isolation via semiprep high-performance liquid chromatography (HPLC) allowed for the simultaneous synthesis of two or three tracers with a total synthesis time of less than 1 h

    Representative immunofluorescence staining images of cytospin slides of healthy peripheral blood M1 and M2 MDM.

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    <p>Immunofluorescence staining of cytospin slides of healthy peripheral blood MDM with the nuclear stain POPO-1 (to confirm presence of cells), TSPO, the macrophage marker CD68 and merging of the three stains. <b>(A)</b> staining of MDM stimulated with LPS plus IFN<i>-</i>γ for 24 hours; <b>(B)</b> staining of MDM stimulated with IL-4 for 24 hours. x400 magnification. Scale bar = 20μm.</p