14 research outputs found

    Effect of intravenous immunoglobulin on the function of Treg cells derived from immunosuppressed mice with <i>Pseudomonas aeruginosa</i> pneumonia

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    <div><p>Aim</p><p>The present study aimed to investigate the effect of intravenous immunoglobulin (IVIG) on regulatory T (Treg) cells derived from immunosuppressed mice with <i>Pseudomonas aeruginosa</i> (PA) pneumonia.</p><p>Methods</p><p>A total of 108 BALB/c mice were randomly divided into the following groups: control group (Control), immunosuppressed group (IS), PA pneumonia group (PA), PA pneumonia in immunosuppressed group (IS + PA), PA pneumonia with IVIG treatment in immunocompetent group (PA + IVIG) and PA pneumonia with IVIG treatment in immunosuppressed group (IS + PA + IVIG). Each group comprised 18 mice. The combined PA pneumonia in immunosuppressed model and the treatment models were established. The mice in each group were sacrificed at 4, 8, and 24 h time points. The general condition and pathological changes in the lung tissues of the mice were monitored. Reverse transcription-polymerase chain reaction was used to detect the forkhead box P3 (<i>FOXP3)</i> mRNA relative expression level in the lung tissues. The enzyme-linked immunosorbent assay was used to detect the serum concentration of active transforming growth factor beta (TGF-╬▓).</p><p>Results</p><p>No inflammatory response were exhibited in the lung tissues of the mice in Control group and IS group, while varying degrees of acute lung injury were revealed in the mice in PA group, IS + PA group, PA + IVIG group and IS + PA + IVIG group. Lung tissue injury was most apparent at the 8 h time point, and it indicated the greatest effect in IS + PA group. Whereas tissue damages were alleviated in PA + IVIG group and IS + PA + IVIG group compared with IS + PA group. In addition, tissue damage lessened in PA + IVIG group compared with PA group and IS + PA + IVIG group. <i>FOXP3</i> mRNA expression levels in the lung tissues and the serum concentration of TGF-╬▓ were lower in IS group, PA group, IS + PA group and IS + PA + IVIG group at the 4, 8 and 24 h time points, respectively compared with Control group. <i>FOXP3</i> mRNA expression levels decreased in PA + IVIG group at the 4h time point and TGF-╬▓ serum concentrations decreased at the 4 and 8h time points compared with Control group, and subsequently increased.</p><p>Conclusions</p><p>In the immunosuppred model with PA pneumonia, the immune system was greatly compromised. IVIG partially restored the immunosuppressed functions of Treg cells, suppressed the overactivated immune system and ameliorated the development of the disease.</p></div

    1.0% agarose gel electrophoresis of total RNA isolated.

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    <p>A, three intact RNA bands for 28S, 18S and 5S RNA. Lane 1, lane 2 and lane 3 in A, B, C, D and E contain 1 ╬╝g of total RNA from <i>D</i>. <i>huoshanense</i> stem, leaf and flower, respectively. A: modified CHAN method; B: original CHAN method; C: Trizol method; D: RNeasy Plant Mini Kit method; E: RNAprep Pure Plant Kit method.</p

    Concentration and purity of total RNA isolated from <i>D</i>.<i>huoshanense</i> stem, leaf and flower using Trizol method.

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    <p>Concentration and purity of total RNA isolated from <i>D</i>.<i>huoshanense</i> stem, leaf and flower using Trizol method.</p

    Comparison of the appearance of CECT and clinical severity of AP.

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    <p>(A) The incidence of ANP in AP patients with AFL or NAFL; (B) The clinical severity of AP in AP patients with AFL or NAFL. CECT: contrast-enhanced computed tomography; AP: acute pancreatitis; AFL: alcoholic fatty liver; NAFL: non- alcoholic fatty liver; AEP: acute edematous pancreatitis; ANP: acute necrotizing pancreatitis; MAP: mild acute pancreatitis; MSAP: moderately severe acute pancreatitis; SAP: severe acute pancreatitis.</p

    Concentration and purity of total RNA isolated from leaves of <i>Salvia miltiorrhiza</i> and <i>Platycodon grandiflorus</i> using modified CHAN method.

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    <p>Concentration and purity of total RNA isolated from leaves of <i>Salvia miltiorrhiza</i> and <i>Platycodon grandiflorus</i> using modified CHAN method.</p

    Agarose gel electrophoresis of the RT-PCR tubulin products.

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    <p>Lane 1, lane 2 and lane 3 showed RT-PCR products from <i>D</i>. <i>huoshanense</i> stem, leaf and flower. Lanes 0 showed RT-PCR product by substituting <i>D</i>. <i>huoshanense</i> cDNA for ddH<sub>2</sub>O. Marker: DL2,000 DNA marker.</p

    Concentration and purity of total RNA isolated from <i>D</i>.<i>huoshanense</i> stem, leaf and flower using original CHAN method.

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    <p>Concentration and purity of total RNA isolated from <i>D</i>.<i>huoshanense</i> stem, leaf and flower using original CHAN method.</p

    Comparison of the appearance of CECT and clinical severity of AP.

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    <p>(A, B) The incidence of ANP in total AP patients with or without FL; (C, D) The clinical severity of AP in total patients with or without FL; (E, F) The incidence of ANP in non-obese AP patients with or without FL; (G, H) The clinical severity of AP in non-obese patients with or without FL; (I, J) The incidence of ANP in obese AP patients with or without FL; (K, L) The clinical severity of AP in obese patients with or without FL; (B, D, F, H, J, L) FL patients were divided according to the severity of FL, including mild FL, moderate FL and severe FL. CECT: contrast-enhanced computed tomography; AP: acute pancreatitis; FL: fatty liver; AEP: acute edematous pancreatitis; ANP: acute necrotizing pancreatitis; MAP: mild acute pancreatitis; MSAP: moderately severe acute pancreatitis; SAP: severe acute pancreatitis.</p

    Concentration and purity of total RNA isolated from <i>D</i>.<i>huoshanense</i> stem, leaf and flower using the modified CHAN method.

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    <p>Concentration and purity of total RNA isolated from <i>D</i>.<i>huoshanense</i> stem, leaf and flower using the modified CHAN method.</p

    <i>FOXP3</i> mRNA relative expression level in lung tissue in each group at 4, 8 and 24h.

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    <p>Six groups were studied: Control group, IS group, PA group, IS + PA group, PA + IVIG group and IS + PA + IVIG group. Immunosuppression was induced on day -5, -3 and -1 by intraperitoneal injection of CYP, PA pneumonia was induced on day 0 and IVIG mediated treatment models were immediately established following PA intervention on day 0. The left lung tissue of the mice was used for testing <i>FOXP3</i> mRNA relative expression by qRT-PCR. qRT-PCR was carried out using a Thermo Fisher PCR kit with FOXP3 and GAPDH primer. <i>FOXP3</i> mRNA relative expression level was calculated by the 2 <sup>(- ΔΔ CT)</sup> method. <i>FOXP3</i> mRNA expression levels in lung tissue in six groups at 4, 8 and 24h were shown.</p
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