2,052 research outputs found

    UTILIZANDO NANOTUBOS DE TiO2 COMO FOTOCATALISADOR NA DEGRADAÇÃO DO CORANTE VERMELHO CONGO

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    Nanotubos de TiO2 foram sintetizados em um sistema hidrot√©rmico. Os nanomateriais foram caracterizados por microscopia eletr√īnica de varredura (MEV) e √°rea superficial. O TiO2 anatase e os nanotubos de TiO2 foram utilizados no processo de fotodegrada√ß√£o do corante vermelho congo. A capacidade de fotodegrada√ß√£o de ambos catalisadores foram comparadas. O corante foi degradado por 90 minutos, utilizando ambos catalisadores, estes apresentaram alta atividade catal√ɬ≠tica pr√≥ximo ao pH 2. A capacidade de fotodegrada√ß√£o do corante utilizando TiO2 anatase e nanotubos de TiO2 em meio i√īnico mostrou-se favor√°vel com a adi√ß√£o de NaCl na concentra√ß√£o de 0,03 mol/L

    Cytogenetics, JAK2 and MPL mutations in polycythemia vera, primary myelofibrosis and essential thrombocythemia

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    BACKGROUND: The detection of molecular and cytogenetic alterations is important for the diagnosis, prognosis and classification of myeloproliferative neoplasms. OBJECTIVE: The aim of this study was to detect the following mutations: JAK2 V617F, JAK2 exon 12 and MPL W515K/L, besides chromosomal abnormalities. Furthermore, molecular and cytogenetic alterations were correlated with the leukocyte and platelet counts, hemoglobin levels and age in all patients and with the degree of fibrosis in primary myelofibrosis cases. METHODS: Twenty cases of polycythemia vera, 17 of essential thrombocythemia and 21 of primary myelofibrosis were selected in the Hematology Department of the Universidade Federal de S√£o Paulo (UNIFESP) between February 2008 and December 2009. The JAK2 V617F, JAK2 exon 12 mutations, MPL W515K and MPL W515L mutations were investigated by real-time PCR and direct sequencing. G-band karyotyping and fluorescence in situ hybridization were used to detect chromosomal abnormalities. RESULTS: Chromosomal abnormalities were observed only in polycythemia vera (11.8%) and primary myelofibrosis cases (17.6%), without correlation to clinical data. Chromosomal abnormalities were not detected by fluorescence in situ hybridization. The JAK2 V617F mutation was observed in polycythemia vera (90%), primary myelofibrosis (42.8%) and essential thrombocythemia (47%). Patients with JAK2 V617F-negative polycythemia vera had lower platelet and leukocyte counts compared to V617F-positive polycythemia vera (p-value = 0.0001 and p-value = 0.023, respectively). JAK2 V617F-positive and MPL W515L-positive primary myelofibrosis cases had a higher degree of fibrosis than V617F-negative cases (p-value = 0.022). JAK2 exon 12 mutations were not detected in polycythemia vera patients. The MPL W515L mutation was observed in one case of primary myelofibrosis and in one of essential thrombocythemia. The MPL W515K mutation was not found in patients with essential thrombocythemia or primary myelofibrosis. The MPL W515L-positive patient with primary myelofibrosis had more severe anemia than other patients with primary myelofibrosis. CONCLUSIONS: This study demonstrates that karyotyping for JAK2 and MPL mutations is useful in the diagnosis of myeloproliferative neoplasms. The precise pathogenetic contribution of these alterations is still unclear. However, this study adds more information about the pathophysiology of polycythemia vera, essential thrombocythemia and primary myelofibrosis.Universidade Federal de S√£o Paulo (UNIFESP) Hematology DepartmentUniversidade Federal de S√£o Paulo (UNIFESP) Rheumatology DepartmentUniversidade Federal de S√£o Paulo (UNIFESP) Genetics DepartmentUNIFESP, Hematology DepartmentUNIFESP, Rheumatology DepartmentUNIFESP, Genetics DepartmentSciEL

    Proveniência e análise sedimentar da porção basal do Grupo Bambuí em Arcos (MG)

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    A origem e evolu√ß√£o da Bacia Bambu√≠ √© tema de grandes debates, em muito intensificados pela recente corrida explorat√≥ria de empresas p√ļblicas e privadas em busca de hidrocarbonetos gasosos. Na por√ß√£o sudoeste da bacia, s√£o raras as oportunidades de se observar o contato basal das rochas sedimentares com seu embasamento, cujas caracter√≠sticas s√£o cruciais para o entendimento dos processos de instala√ß√£o da bacia e do in√≠cio de seu preenchimento. Por meio de testemunhos de sondagem, teve-se acesso a toda a por√ß√£o basal do Grupo Bambu√≠ na regi√£o de Arcos (MG), incluindo seu embasamento. A se√ß√£o amostrada exibe como embasamento um granodiorito arqueano (c. 2,8 - 2,9 Ga), fortemente fraturado √† √©poca da sedimenta√ß√£o. A unidade basal do Grupo Bambu√≠ √© um tilito de alojamento maci√ßo, polim√≠tico, de espessura decim√©trica. Gr√£os de zirc√£o separados da matriz do tilito foram datados e exibem um pico principal de idades em c. 2,8 Ga, indicando proveni√™ncia do pr√≥prio embasamento. Sobre o tilito repousam calc√°rios impuros, que passam gradativamente para um pacote pel√≠tico, em tend√™ncia de preenchimento retrogradacional. Fragmentos terr√≠genos no calc√°rio impuro sugerem que o embasamento tenha continuado a atuar como fonte durante a sedimenta√ß√£o dos metros iniciais da se√ß√£o. As rochas pel√≠ticas que ocorrem para o topo t√™m assinatura litoqu√≠mica compat√≠vel com fontes √°cidas, e possuem idades-modelo Sm-Nd de 1,7 Ga. O acervo de dados sugere que rochas da Faixa Bras√≠lia tenham atuado como fonte para as rochas pel√≠ticas, corroborando a interpreta√ß√£o de um car√°ter foreland para a bacia.The origin and evolution of Bambu√≠ Basin has been a matter of debate, in much intensified by the recent exploratory efforts carried out by public and private companies looking for natural gas. In the southeastern portion of the basin there are rare opportunities to access the contact between the sedimentary rocks and its basement, whose characteristics are crucial to understanding the processes of basin installation and the early sedimentation. The analysis of drill cores allowed us to describe the lowermost Bambu√≠ Group in Arcos (MG) region, including its basement. The sampled section displays as basement an archean granodiorite (ca. 2.8 - 2.9 Ga), fractured at the time of sedimentation. The Bambu√≠ Group basal unit is dm-thick, massive lodgment tillite. Grains of zircon separated from the tillite matrix were dated and show a main age peak at ca. 2.8 Ga, indicating provenance from the own basement. On the tillite rests an impure limestone that passes gradually to a muddy unit, in a retrogradational filling trend. Terrigenous fragments in the impure limestone suggests that the basement has continued to be a source of sediments in the bottom of the section. The pelitic rocks that occurs to the top has a litochemical signature compatible with acidic source rocks, and shows Sm-Nd model ages of 1.7 Ga. These data suggests that rocks from the Bras√≠lia Belt have acted as a source for the pelitic rocks, supporting the interpretation of a foreland set for the basin

    Potentially malignant oral lesions: clinical and morphological analysis of 205 cases

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    Silveira , E. J. D. et al. Les√Ķes orais com potencial de maligniza√ß√£o: an√°lise cl√≠nica e morfol√≥gica de 205 casos. J. Bras. Patol. Med. Lab., v. 45, n. 3, p. 233-238, jun 2009. ISBN 1676-2444.abstract Objective: The objective of this study was to perform a clinical and morphological analysis of potentially malignant oral lesions. Material and method: The clinical and morphological data related to these lesions were obtained from clinical and histopathological records, respectively. Results: Out of 205 cases, the most prevalent lesion was oral leukoplakia (70.7%), followed by actinic cheilitis (16.1%), erythroplakia (9.8%) and erythroleukoplakia (3.4%). There was a significant association between the lesions and gender (p < 0.001). The female gender was the most affected in leukoplakias and erythroplakias. Caucasians were mostly affected in all conditions. As far as anatomical location is concerned, the alveolar ridge was the most prevalent site in oral leukoplakias (21%); the palate was the most affected in erythroplakia (47.4%) and erythroleukoplakia (57.1%). There was no significant difference among the lesions as to age (p > 0.05), although they were more frequent in the 5th, 6th and 7th decades of life. In most cases of erythroplakias and erythroleukoplakias, it was observed that clinical and morphological diagnoses were incompatible. There was a significant association between malignant transformation and lesions (p < 0.001) as the erythroplakia presented a malignization rate of 20%, whereas in leukoplakias the rate was 2.1%. Conclusion: The prevalence of potentially malignant oral lesions was similar to other findings in the medical literature, but with some differences in their epidemiological profile. It is important to highlight that these entities require further medical investigation, mainly erythroplakias, which showed high risk of malignization and the highest rate of clinical misdiagnosis RESUME: Objetivos: O objetivo desta pesquisa foi realizar uma an√°lise cl√≠nica e morfol√≥gica em les√Ķes orais com potencial de maligniza√ß√£o. Material e m√©todo: Os dados cl√≠nicos e morfol√≥gicos referentes a tais les√Ķes foram obtidos a partir das fichas cl√≠nicas e dos laudos histopatol√≥gicos, respectivamente. Resultados: Dos 205 casos analisados, a les√£o mais prevalente foi a leucoplasia (70,7%), seguida da queilite act√≠nica (16,1%), eritroplasia (9,8%) e eritroleucoplasia (3,4%). Verificou-se uma associa√ß√£o significativa entre o sexo e as les√Ķes (p < 0,001), sendo o sexo feminino mais prevalente nas leucoplasias e eritroplasias. A ra√ßa branca foi mais acometida em todas as les√Ķes. Quanto √† localiza√ß√£o anat√īmica, o rebordo alveolar prevaleceu na leucoplasia (21%), na eritroplasia e eritroleucoplasia, o palato foi mais acometido com 47,4% e 57,1%, respectivamente. Com rela√ß√£o √† idade, n√£o houve diferen√ßa significativa entre as les√Ķes (p > 0,05), sendo mais frequentes nas quinta, sexta e s√©tima d√©cadas de vida. Observou-se que, na maioria dos casos de eritroplasias e eritroleucoplasias, os diagn√≥sticos cl√≠nicos foram incompat√≠veis com o morfol√≥gico. Verificou-se associa√ß√£o significativa entre transforma√ß√£o maligna e les√Ķes (p < 0,001), pois a eritroplasia apresentou uma taxa de maligniza√ß√£o de 20%, enquanto na leucoplasia o √≠ndice foi de 2,1%. Conclus√£o: A preval√™ncia das les√Ķes orais com potencial de maligniza√ß√£o foi semelhante ao encontrado na literatura, mas com algumas diferen√ßas em rela√ß√£o ao perfil epidemiol√≥gico das mesmas, ressaltando-se que estas entidades merecem uma aten√ß√£o especial por parte do cl√≠nico, especialmente as eritroplasias, que apresentaram um elevado percentual de maligniza√ß√£o e o maior √≠ndice de equ√≠vocos no diagn√≥stico cl√≠nic

    Influ√™ncia da Temperatura do Solo no Efluxo de Co2 do Solo em uma Floresta Tropical da Amaz√īnia Oriental

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    It was studied the variability of the average hourly flux of CO2associated with the soil temperature and soil moisture in an area of tropicalrain forest in Amazonia, in the experimental site of the NationalForest Caxiuan√£, State of Par√°. Purpose was to evaluate the influence oftemperature and soil moisture on CO2 efflux from soil and estimate thevalue of Q10. The soil temperature did not get a good correlation withsoil CO2 efflux.Estudou-se a variabilidade m√©dia hor√°rio do fluxo de CO2 dosolo associado √† temperatura e umidade do solo em uma √°rea de florestatropical chuvosa na Amaz√īnia, no s√≠tio experimental na Floresta Nacionalde Caxiuan√£, no Estado do Par√°. Objetivo foi avaliar a influ√™ncia datemperatura e umidade do solo no efluxo de CO2 do solo e estimar ovalor do Q10. A temperatura do solo n√£o obteve uma boa correla√ß√£o comefluxo de CO2 do solo

    Adult-onset opsoclonus-myoclonus-ataxia syndrome as a manifestation of brazilian lyme disease-like syndrome: a case report and review of literature

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    Described in 1962, the opsoclonus-myoclonus-ataxia syndrome (OMAS) is a rare, neurologically debilitating disorder with distinct characteristics that may begin in childhood or adult life. Although many cases remain without etiological diagnosis, others are related to neoplasms and infectious diseases. We report a 41-year-old previously healthy male with an 8-day history of headache, vertigo, nausea, vomiting, and nystagmus. After a normal brain computed tomography and lymphocytic pleocytosis in cerebral spinal fluid (CSF), intravenous acyclovir therapy was initiated in the emergency room. On the third day of hospitalization, the diagnosis of OMAS was made based on the presence of chaotic and irregular eye movements, dysarthric speech, gait instability, generalized tremor, and myoclonic jerks. In the face of his neurological worsening, ampicillin followed by nonspecific immunotherapy (methylprednisolone and intravenous immunoglobulin) was prescribed, with mild clinical improvement. After a thorough laboratory workup, the definite diagnosis of neuroborreliosis was established and ceftriaxone (4 g/daily/3wks) and doxycycline (200 mg/day/2 mo) was administered. Toward the end of the ceftriaxone regimen, the neurologic signs substantially improved. We believe this to be the first case description of OMAS as clinical presentation of Brazilian Lyme disease-like syndrome (Baggio-Yoshinari syndrome)
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