Multilocus sequence typing of a global collection of pasteurella multocida isolates from cattle and other host species demonstrates niche association

Background- Pasteurella multocida causes disease in many host species throughout the world. In bovids, it contributes to bovine respiratory disease (BRD) and causes haemorrhagic septicaemia (HS). Previous studies have suggested that BRD-associated P. multocida isolates are of limited diversity. A multilocus sequence typing (MLST) scheme for P. multocida was used to determine whether the low levels of diversity reported are due to the limited discriminatory power of the typing method used, restricted sample selection or true niche association. Bovine respiratory isolates of P. multocida (n = 133) from the UK, the USA and France, collected between 1984 and 2008 from both healthy and clinically affected animals, were typed using MLST. Isolates of P. multocida from cases of HS, isolates from other host species and data from the MLST database were used as comparison. Results - Bovine respiratory isolates were found to be clonal (ISA 0.45) with 105/128 belonging to clonal complex 13 (CC13). HS isolates were not related to bovine respiratory isolates. Of the host species studied, the majority had their own unique sequence types (STs), with few STs being shared across host species, although there was some cross over between porcine and bovine respiratory isolates. Avian, ovine and porcine isolates showed greater levels of diversity compared to cattle respiratory isolates, despite more limited geographic origins. Conclusions - The homogeneity of STs of bovine respiratory P. multocida observed, and the differences between these and P. multocida subpopulations from bovine non-respiratory isolates and non-bovine hosts may indicate niche association

\u3ci\u3eTheileria electrophori\u3c/i\u3e n.sp., a Parasite of the Electric Eel \u3ci\u3eElectrophorus electricus\u3c/i\u3e (Osteichthyes: Cypriniformes: Gymnotidae) from Amazonian Brazil

The name Theileria electrophori n. sp. is proposed for a small parasite described in the erythrocytes of the electric eel, Electrophorus electricus, from Amazonian Brazil. Division of the organism in the erythrocyte produces only four bacilliform daughter cells which become scattered in the host cell, without a cruciform or rosette-shaped disposition. Exoerythrocytic meronts producing a large number of merozoites were encountered in Giemsa-stained impression smears of the internal organs, principally in the liver, and are presumably the source of the intraerythrocytic forms of the parasite. This developmental pattern is characteristic of piroplasms within the family Theileriidae, where the author considers the parasite of E. electricus to most appropriately belong. It effectively distinguishes the organism from the dactylosomatid parasites Babesiosoma Jakowska and Nigrelli, 1956 and Dactylosoma Labbé, 1894 also found in fishes. This appears to be the second report of Theileria Bettencourt, Franca and Borges, 1907 in a fish

An instructional development project in grade two : the theme of friendship

The theme of friendship was extended through quality literature experiences and related expressive activities to second grade children at the beginning of the school year. The primary goals were to involve children in the language processes and to develop a community of friendly learners. Teacher-directed and student-initiated activities were provided. The student-initiated activities were presented as options through literature-based learning centers. The goals of the centers included ways to make friends and to be a friend, an understanding that friends can be of all ages, involvement in pals, ways to cope with difficulties encountered in friendships and to recognize each others\u27 individuality, and an appreciation of animals as friends

Draft Genome Sequences of Strains of Pasteurella multocida Isolated from the United Kingdom and the United States

Pasteurella multocida is a major pathogen of farm animals and has worldwide distribution. Here we report the draft genome sequences of four strains that were isolated from animals in the United Kingdom and the United States and represent pathogenic and commensal presentation of the bacterium

Being both narrative practitioner and academic researcher: A reflection on what thematic analysis has to offer narratively informed research

What opportunities are there for narrative practitioners to engage in academic research whilst retaining an alignment with poststructuralist ideas, feminist commitments and narrative practice principles? This paper considers Virginia Braun and Victoria Clarke’s model of thematic analysis (TA) as an approach which can overcome some of the tensions that arise when integrating both narrative practitioner and researcher stance. Drawing on one practitioner-researcher’s experience of navigating some of these dilemmas and incorporating a rich discussion of some of the heritages, understandings and intentions that underpin TA and its development, this paper seeks to assist, inform and encourage narrative practitioners who are reaching for approaches that offer a good fit for their research hopes and aims

Simulation Study Using a New Type of Sample Variance

We evaluate with simulated data a new type of sample variance for the characterization of frequency stability. The new statistic (referred to as TOTALVAR and its square root TOTALDEV) is a better predictor of long-term frequency variations than the present sample Allan deviation. The statistical model uses the assumption that a time series of phase or frequency differences is wrapped (periodic) with overall frequency difference removed. We find that the variability at long averaging times is reduced considerably for the five models of power-law noise commonly encountered with frequency standards and oscillators