The Evolution of Lyman alpha Absorbers in the Redshift Range 0.5<z<1.9

We investigate the evolution and the statistical properties of the Lya absorbers of the intergalactic medium (IGM) in the largely unexplored redshift range z=0.5-1.9. We use high-resolution (R > 30,000) UV (STIS) and optical (VLT/UVES and Keck/HIRES) spectra of nine bright quasars with z_em < 1.94. The main results for the combined Lya line sample are summarized as follows: 1. The evolution of the number density of the absorbers can be described by the power law dn/dz ~ (1+z)^gamma. The number density of the low column density lines decreases with decreasing z with gamma=0.74+-0.31 in the interval z=0.7-1.9. A comparison with results at higher redshifts shows that it is decelerated in the explored redshift range and turns into a flat evolution for z -> 0. The stronger absorbers thin out faster (gamma=1.50+-0.45). The break in their evolution predicted for z=1.5-1.7 cannot be seen down to z=0.7. On the other hand, a comparison with values from the literature for the local number density gives a hint that this break occurs at lower redshift. 2. The distribution of the column densities of the absorbers is complete down to N_HI=10^12.90 cm^-2. It can be approximated by a single power law with the exponent beta=1.60+-0.03 over almost three orders of magnitude. beta is redshift independent. 3. The Lya lines with lower column densities as well as the higher column density lines show marginal clustering with a 2 sigma significance over short distances (Delta v < 200 km/s and Delta v < 100 km/s, respectively). We do not see any difference in the clustering with either column density or redshift.Comment: 15 pages, 18 figures, accepted for publication in Astronomy & Astrophysic

Energy-saving wastewater treatment systems : formulation of cost functions

Natural interactions between water, soil, atmosphere, plants and microorganisms include physical, chemical and biological processes with decontaminating capacities. Natural or energy-saving wastewater treatment systems utilize these processes and thereby enable a sustainable management in the field of wastewater treatment, offering low investment and operation costs, little or no energy consumption, little and low-skill labor requirements, good landscape integration and excellent feasibility for small settlements, especially when remote from centralized sewer systems. The objective of this work is the development of cost functions for investment and operation of energysaving wastewater treatment technologies. Cost functions are essential for making cost estimations based on a very reduced number of variables. The latter are easily identified and quantified and have a direct bearing on the costs in question. The formulated investment and operation cost functions follow a power law, and the costs decrease with the increase of the population served. The different energy-saving wastewater treatment systems serving small population settlements, between 50 p.e. and 250 p.e., present associated investment costs varying from 400 e/p.e. to 200 e/p.e. and annual operation costs in the range of 70 €/p.e. to 20 €/p.e., respectively.(undefined

Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system

The production and characterization of recombinant antibodies to aflatoxin B[SUB1] (AFB[SUB1]), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor (BIAcore), which measures biomolecular interactions in 'real-time'. Single chain Fv (scFv) antibodies were generated to aflatoxin B1 from an established phage display system, which incorporated a range of different plasmids for efficient scFv expression. The scFv's were used in the development of a competitive ELISA, and also for the development of surface plasmon resonance (SPR)-based inhibition immunoassays. They were found to be suitable for the detection of AFB[SUB1], in this format, with the assays being sensitive and reproducible

Nitrogen-deficient and iron-rich associated absorbers with oversolar metallicities towards the quasar HE0141-3932

HE0141-3932 (zem=1.80) is a bright blue radio-quite quasar which reveals an emission line spectrum with an unusually weak Ly-alpha line. In addition, large redshift differences (Delta z=0.05) are observed between high ionization and low ionization emission lines. Absorption systems identified at z=1.78, 1.71, and 1.68 show mild oversolar metallicities (Z ~= 1-2Zsolar) and can be attributed to the associated gas clouds ejected from the circumnuclear region. The joint analysis of the emission and absorption lines leads to the conclusion that this quasar is seen almost pole-on. Its apparent luminosity may be Doppler boosted by ~10 times. The absorbing gas shows high abundance of Fe, Mg, and Al ([Fe, Mg, Al/C] ~= 0.15+/-0.10) along with underabundance of N ([N/C]<=-0.5). This abundance pattern is at variance with current chemical evolution models of QSOs predicting [N/C]>0 and [Fe/C]<0 at Z ~ Zsolar. Full details of this work are given in Reimers et al. (2005).Comment: 2 pages, to appear in the Proceed. of IAU Symp.228 "From Lithium to Uranium: Elemental Tracers of Early Cosmic Evolution", eds. V.Hill, P.Francois and F.Prima

Opposing effects of Elk-1 multisite phosphorylation shape its response to ERK activation.

Multisite phosphorylation regulates many transcription factors, including the serum response factor partner Elk-1. Phosphorylation of the transcriptional activation domain (TAD) of Elk-1 by the protein kinase ERK at multiple sites potentiates recruitment of the Mediator transcriptional coactivator complex and transcriptional activation, but the roles of individual phosphorylation events had remained unclear. Using time-resolved nuclear magnetic resonance spectroscopy, we found that ERK2 phosphorylation proceeds at markedly different rates at eight TAD sites in vitro, which we classified as fast, intermediate, and slow. Mutagenesis experiments showed that phosphorylation of fast and intermediate sites promoted Mediator interaction and transcriptional activation, whereas modification of slow sites counteracted both functions, thereby limiting Elk-1 output. Progressive Elk-1 phosphorylation thus ensures a self-limiting response to ERK activation, which occurs independently of antagonizing phosphatase activity