138 research outputs found

    Vertically well-aligned ZnO nanorods based biosensor for determination of paeoniflorin as an antidepressant medicine

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    In the study, a novel method for detecting paeoniflorin (PF), a significant antidepressant drug, is described that makes use of vertically aligned zinc oxide nanorods (ZnO NRs). Vertically aligned zinc oxide (ZnO) nanorods and rGO were electrodeposited on the GCE electrode surface, according to structural investigations performed using field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). According to electrochemical experiments, ZnO NRs and rGO increase the electron transfer rate and support catalytic activity, making it easier to detect and quantify PF in the electrochemical sensor. The results exhibited that the linear range and sensitivity of ZnO-rGO/GCE were, respectively, 0.1–1305 μg/mL and 0.03792 μA/μg.mL−1. The PF sensor also achieved a low detection limit of 3.4 ng/mL. It was investigated to see how well a PF sensor worked to analyze a sample of human serum. Relative standard deviations (RSD) for sample detections for the proposed PF sensor were all below 4.13 %, according to analytical analyses utilizing the conventional addition approach, with recoveries ranging from 96.00 % to 99.25 %. These findings affirm that the proposed PF sensor exhibits a high degree of accuracy, rendering it well-suited for clinical applications and the analysis of human bodily fluids

    Multi-omics analysis of human mesenchymal stem cells shows cell aging that alters immunomodulatory activity through the downregulation of PD-L1

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    Abstract Mesenchymal stem cells (MSCs) possess potent immunomodulatory activity and have been extensively investigated for their therapeutic potential in treating inflammatory disorders. However, the mechanisms underlying the immunosuppressive function of MSCs are not fully understood, hindering the development of standardized MSC-based therapies for clinical use. In this study, we profile the single-cell transcriptomes of MSCs isolated from adipose tissue (AD), bone marrow (BM), placental chorionic membrane (PM), and umbilical cord (UC). Our results demonstrate that MSCs undergo a progressive aging process and that the cellular senescence state influences their immunosuppressive activity by downregulating PD-L1 expression. Through integrated analysis of single-cell transcriptomic and proteomic data, we identify GATA2 as a regulator of MSC senescence and PD-L1 expression. Overall, our findings highlight the roles of cell aging and PD-L1 expression in modulating the immunosuppressive efficacy of MSCs and implicating perinatal MSC therapy for clinical applications in inflammatory disorders

    MCCFNet: multi-channel color fusion network for cognitive classification of traditional Chinese paintings.

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    The computational modeling and analysis of traditional Chinese painting rely heavily on cognitive classification based on visual perception. This approach is crucial for understanding and identifying artworks created by different artists. However, the effective integration of visual perception into artificial intelligence (AI) models remains largely unexplored. Additionally, the classification research of Chinese painting faces certain challenges, such as insufficient investigation into the specific characteristics of painting images for author classification and recognition. To address these issues, we propose a novel framework called multi-channel color fusion network (MCCFNet), which aims to extract visual features from diverse color perspectives. By considering multiple color channels, MCCFNet enhances the ability of AI models to capture intricate details and nuances present in Chinese painting. To improve the performance of the DenseNet model, we introduce a regional weighted pooling (RWP) strategy specifically designed for the DenseNet169 architecture. This strategy enhances the extraction of highly discriminative features. In our experimental evaluation, we comprehensively compared the performance of our proposed MCCFNet model against six state-of-the-art models. The comparison was conducted on a dataset consisting of 2436 TCP samples, derived from the works of 10 renowned Chinese artists. The evaluation metrics employed for performance assessment were Top-1 Accuracy and the area under the curve (AUC). The experimental results have shown that our proposed MCCFNet model significantly outperform all other benchmarking methods with the highest classification accuracy of 98.68%. Meanwhile, the classification accuracy of any deep learning models on TCP can be much improved when adopting our proposed framework

    Multicropping Pattern Reveals Human Adaptation at the Wanbei Site (ca. 5.7–4.4 ka cal. BP) in the Middle and Lower Huai River Valley, China

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    The middle and lower Huai River Valley, located between the Yangtze and Yellow Rivers, was a key transitional zone for the northward spread of rice and southward migration of millet agriculture in central-eastern China during the Holocene. Knowing when millets spread here, how they were combined with rice in mixed farming, the reasons for their spread, and the temporal variation of cropping patterns is of crucial significance to the development of our understanding of ancient adaptation strategies adopted by human societies in response to climatic and cultural changes. Focusing on crops, phytolith analyses of the soil samples, in tandem with radiocarbon dating from the Wanbei site, reveal evidence of a multicropping pattern of combining rice (Oryza sative), broomcorn millet (Panicum miliaceum), and foxtail millet (Setaria italica) during the Dawenkou culture period between 5720 and 4426 cal. BP in the middle and lower Huai River Valley, China. The data show that rice was always the principal crop of the pattern, and that domesticated rice was developed during the early and middle Dawenkou culture periods. However, its domestication rate became lower during the late Dawenkou culture period. Broomcorn millet and foxtail millet with domesticated traits appeared only in lower proportions of the total produced throughout the period. The proportions of rice and foxtail millet increased slightly, while the proportions of broomcorn millet decreased over time. Finally, the formation of the multicropping pattern at Wanbei may have been primarily influenced by both the warm and wet climatic environment and the cultural exchange and communication between the Haidai region and the middle and lower Huai River Valley during the Dawenkou culture period. The findings in this paper may not only contribute to mapping the spatiotemporal route for the northward expansion of rice agriculture and southward spread of millet agriculture, but also assist in understanding the human adaptation strategies employed in eastern China during the Holocene

    Sciences for The 2.5-meter Wide Field Survey Telescope (WFST)

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    The Wide Field Survey Telescope (WFST) is a dedicated photometric survey facility under construction jointly by the University of Science and Technology of China and Purple Mountain Observatory. It is equipped with a primary mirror of 2.5m in diameter, an active optical system, and a mosaic CCD camera of 0.73 Gpix on the main focus plane to achieve high-quality imaging over a field of view of 6.5 square degrees. The installation of WFST in the Lenghu observing site is planned to happen in the summer of 2023, and the operation is scheduled to commence within three months afterward. WFST will scan the northern sky in four optical bands (u, g, r, and i) at cadences from hourly/daily to semi-weekly in the deep high-cadence survey (DHS) and the wide field survey (WFS) programs, respectively. WFS reaches a depth of 22.27, 23.32, 22.84, and 22.31 in AB magnitudes in a nominal 30-second exposure in the four bands during a photometric night, respectively, enabling us to search tremendous amount of transients in the low-z universe and systematically investigate the variability of Galactic and extragalactic objects. Intranight 90s exposures as deep as 23 and 24 mag in u and g bands via DHS provide a unique opportunity to facilitate explorations of energetic transients in demand for high sensitivity, including the electromagnetic counterparts of gravitational-wave events detected by the second/third-generation GW detectors, supernovae within a few hours of their explosions, tidal disruption events and luminous fast optical transients even beyond a redshift of 1. Meanwhile, the final 6-year co-added images, anticipated to reach g about 25.5 mag in WFS or even deeper by 1.5 mag in DHS, will be of significant value to general Galactic and extragalactic sciences. The highly uniform legacy surveys of WFST will also serve as an indispensable complement to those of LSST which monitors the southern sky.Comment: 46 pages, submitted to SCMP

    Effects of Long-Term Low-Protein Diets Supplemented with Sodium Dichloroacetate and Glucose on Metabolic Biomarkers and Intestinal Microbiota of Finishing Pigs

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    The objective of this study was to evaluate the effects of low-protein (LP) diets supplemented with sodium dichloroacetate (DCA) and glucose (GLUC) on metabolic markers and intestinal microbiota of finishing pigs. A total of 80 crossbred growing barrows were allocated randomly to one of the five treatments, including the normal protein level diet (CON), the LP diets, LP with 120 mg/kg DCA (LP + DCA) or 1.8% glucose (LP + GLUC), and LP with 120 mg/kg DCA and 1.8% glucose (LP + DCA + GLUC). The LP diet increased the plasma HDL, triglyceride, and cholesterol concentrations and reduced the bile acid, urea nitrogen, albumin, and total protein concentrations compared to the CON diet (p p p < 0.05). Moreover, the LP diets with or without DCA and GLUC supplementation increased the relative abundance of colonic microbiota related to carbohydrate fermentation in finishing pigs. In conclusion, 120 mg/kg DCA or 1.8% GLUC supplementation in an LP diet modulated the hepatic lipid metabolism of pigs, while the DCA along with GLUC supplementation likely improved the lipid metabolism by stimulating bile acid secretion

    Revealing the Regulatory Mechanism of lncRNA-LMEP on Melanin Deposition Based on High-Throughput Sequencing in Xichuan Chicken Skin

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    The therapeutic, medicinal, and nourishing properties of black-bone chickens are highly regarded by consumers in China. However, some birds may have yellow skin (YS) or light skin rather than black skin (BS), which causes economic losses every year. Long noncoding RNAs (lncRNAs) are widely present in living organisms, and they perform various biological functions. Many genes associated with BS pigmentation have been discovered, but the lncRNAs involved and their detailed mechanisms have remained untested. We detected 56 differentially expressed lncRNAs from the RNA-seq of dorsal skin (BS versus YS) and found that TCONS_00054154 plays a vital role in melanogenesis by the combined analysis of lncRNAs and mRNAs. We found that the full length of the TCONS_00054154 sequence was 3093 bp by RACE PCR, and we named it LMEP. Moreover, a subcellular localization analysis identified that LMEP is mainly present in the cytoplasm. After the overexpression and the interference with LMEP, the tyrosinase content significantly increased and decreased, respectively (p &lt; 0.05). In summary, we identified the important lncRNAs of chicken skin pigmentation and initially determined the effect of LMEP on melanin deposition

    Theory and Design of Feasible Active Noise Control Systems for 3D Regions

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    This thesis advances Active Noise Control (ANC) over three-dimensional (3D) space using feasible loudspeaker and microphone array systems. By definition, ANC reduces unwanted acoustic noise by generating an anti-noise signal(s) from secondary loudspeakers. The concept of spatial ANC aims to reduce unwanted acoustic noise over a continuous 3D region, by utilizing multiple microphones and multiple secondary loudspeakers to create a large-sized quiet zone for listeners in three-dimensional space. However, existing spatial ANC techniques are usually impractical and difficult to implement due to their strict hardware requirements and high computation complexity. Therefore, this thesis explores various aspects of spatial ANC, seeking algorithms and techniques to promote the reliability and feasibility of ANC over space in real-life applications. The spherical harmonic analysis technique is introduced as the basis of conventional spatial ANC systems. This technique provides an accurate representation of a given spatial sound field using higher-order microphone (spherical microphone array) recordings. Hence, the residual noise field in a spatial ANC system can be effectively captured spatially by applying the spherical harmonic technique. Incorporating conventional spatial ANC methods, we developed a series of algorithms and methods that optimize conventional methods regarding array geometries and ANC algorithms, towards improving the feasibility of a conventional spatial ANC system involving the spherical harmonic analysis. Overall, motivated by feasible and realistic designs for spatial ANC systems, work included in this thesis mainly solves the three problems of: (i) the impracticality of realizing spherical microphone and loudspeaker arrays, (ii) achieving secondary channel estimation with microphones remote from their desired locations, and (iii) unreasonable delays inherent to frequency domain spatial ANC methods. Based on our work, we have stepped towards achieving a spatial ANC system in a real-world environment for people to enjoy silence in the control region with the reliable usage of resources and algorithms. Several contributions of this work are: (i) designing a 3D spatial ANC system using multiple circular microphone and loudspeaker arrays instead of spherical arrays, (ii) proposing a 3D spatial ANC method with remote microphone technique such that noise reduction over a region is achieved with microphones remote from the region, (iii) proposing a secondary channel estimation method using a moving higher-order microphone such that usage of an error microphone array is not necessary, (iv) deriving a time domain spherical harmonic analysis method for open spherical microphone array recording with less delay than in the frequency domain, and (v) designing a feed-forward adaptive spatial ANC algorithm incorporating the time domain spherical harmonic analysis technique to better minimize the noise in the region of interest

    Livelihood Sustainability of Herder Households in North Tibet, China

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    The livelihood sustainability of rural residents has attracted a great deal of attention across the globe, especially in remote mountain areas. In this study, we interviewed 696 householders who were randomly selected from &lsquo;Changtang&rsquo; (also called &lsquo;North Tibet&rsquo;), Tibetan Autonomous Region, China. Under the sustainable livelihood index (SLI) framework, we evaluated the livelihood sustainability of herder households and examined the differences between low- and high-sustainability groups. Our findings revealed the following: (1) The livelihood sustainability of all herder households was generally low; low-sustainability households accounted for 87.07% of the samples. (2) Social, human, and physical capitals accounted for 81.6% of the variance in the SLI of herder households. Less physical capital was the main reason for a lower SLI. (3) Compared with high-SLI households, low-SLI families were characterized by having a higher dependency on natural resources, fewer fixed assets, weaker personal ability, and less participation in social organizations. Moreover, low-sustainability households were more vulnerable to medical expenses. Our study suggests that policymakers should pay closer attention to skills training, promote livelihood diversification, and strengthen social capital security. These actions are recommended for global poverty reduction and to promote the United Nations&rsquo; Sustainable Development Goals

    Data_Sheet_1_Association between plasma selenium and risk of ischemic stroke: A community-based, nested, and case-control study.docx

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    BackgroundThe prospective association between plasma Se and stroke risk remains inconclusive. The relationship between Se and ischemic stroke among a low circulating Se status population deserves more attention, especially for Chinese people who were a high-risk group for Se deficiency.ObjectiveThe relationship between plasma Se concentration and ischemic stroke risk in a large-scale Chinese community-based population and any potential effect modifiers were investigated.MethodsA nested, case-control study, using data from the “China H-type Hypertension Registry Study” were conducted. A total of 1,904 first ischemic stroke cases and 1,904 controls matched for age, sex, and village were included in this study. The association between plasma Se and first ischemic stroke was evaluated by conditional logistic regression analyses.ResultsThe median value of plasma Se was 65.8 μg/L among total participants. Overall, a significant inverse relationship between plasma Se and first ischemic stroke risk was found (per SD increment; adjusted OR: 0.87; 95% CI: 0.80 and 0.95). Accordingly, a significantly lower risk of first ischemic stroke was found in participants in quartile 3 (65.8−ConclusionPlasma Se was significantly inversely associated with the risk of first ischemic stroke among a large-scale Chinese community-based population (most adults with hypertension and elevated total homocysteine), especially among those with lower LDL-C and lower homocysteine levels.</p