38 research outputs found

    Assessing Students┬┤ Needs in English Langugae Teaching

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    The present article is based on the analysis of the data gathered in a questionnaire survey conducted in the selected groups of the 1st year students who began studying English at the School of Business Administration in Karvin├í in the summer semester of the academic year 2015-2016. The goal of the study is to detect studentsÔÇÖ needs and requirements concerning their foreign language (FL) study at SBA in Karvin├í. The data collecting was carried out by distributing questionnaires to respondents and evaluating them. The evaluation of the answers has shown that there exists a discrepancy between students' awareness of what their command of English should be and what their future employers would expect and their lack of motivation

    Advantages and Disadvantages of Online Teaching and Learning Business English Based on a Survey Conducted among SBA Students

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    The article presents results of a survey conducted using the method of the Semantic Differential among students of Business English at School of Business Administration in Karviná, Silesian University in Opava, related to respondents´ attitudes to advantages and disadvantages of online learning during the first part of the Coronavirus pandemic.  Students´ answers have shown the lack of unanimous responses, which indicates the fact that they were not able to evaluate the situation in a distinct way placing their answers mainly in the middle of the offered spectrum. The authors of the article especially appreciate the data collected in the two last questions as they can contribute to optimization of online learning and teaching in the future

    Students┬┤ Attitudes to Cultural and Religious Diversity and Changing Conditions on Labour Market and in Education System

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    The presented paper deals with the questionnaire survey conducted by means of the Semantic Differential Method in the groups of students studying at School of Business Administration in Karviná, Silesian University in Opava. The research focuses on students´ attitudes towards some controversial aspects of the globalized society characterized by migration and plurality trends. The evaluation of the respondents´ opinions is especially related to the areas of religious and cultural diversity as well as the education system and its ability to adequately react to the current changes. Students´ answers also show their attitudes to the ethnic diversity at a potential workplace and focus on professional intercultural communication and problems that might occur in it. The paper also involves a short comparison of selected responses in the surveys conducted in 2016 and 2020.

    Vitamin C Enhances the Generation of Mouse and Human Induced Pluripotent Stem Cells

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    SummarySomatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. However, the low efficiency and slow kinetics of the reprogramming process have hampered progress with this technology. Here we report that a natural compound, vitamin C (Vc), enhances iPSC generation from both mouse and human somatic cells. Vc acts at least in part by alleviating cell senescence, a recently identified roadblock for reprogramming. In addition, Vc accelerates gene expression changes and promotes the transition of pre-iPSC colonies to a fully reprogrammed state. Our results therefore highlight a straightforward method for improving the speed and efficiency of iPSC generation and provide additional insights into the mechanistic basis of the reprogramming process

    Multicultural team conflict management

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    The article presents the potential problems related to conflict resolution while cooperating in multicultural teams. Special attention is paid to specific character of such teams as well as to the concept of productive conflict and the ways of resolving it. The experiences gained in the Erasmus Intenstive Programme - Effective Working in Multicultural Teams were used

    Molecularly Engineered PEG-TRAP Conjugates for Platelet Activation and Accelerated Hemostasis

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    Synthetic peptide conjugates that can molecularly target and activate blood platelets are designed for hemostasis applications. An engineered thrombin receptor-activating peptide (TRAP-Cys, SFLLRNPNC) is covalently attached to cell-compatible 8-arm poly (ethylene glycol) maleimides via Michael addition. It is proved that the ability of TRAP to activate platelets is retained in the conjugated form by means of thromboelastometry, platelet aggregation assay and flow cytometry. A 0.5 % PEG-TRAP solution can efficiently activate platelets and shorten the clotting time to ~45 %

    Correction to: The role of fibrinolysis inhibition in engineered vascular networks derived from endothelial cells and adipose-derived stem cells

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    The original article [1] contains numerous value errors in the graphs in Fig. 2b regarding the markers describing the values for total tubule length and mean tubule length without aprotinin at 2.5 mg/ml concentration of fibrinogen. The corrected version of this figure can be viewed ahead

    The role of fibrinolysis inhibition in engineered vascular networks derived from endothelial cells and adipose-derived stem cells

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    Abstract Background Co-cultures of endothelial cells with mesenchymal stem cells currently represent one of the most promising approaches in providing oxygen and nutrient supply for microvascular tissue engineering. Still, to translate this model into clinics several in vitro parameters including growth medium and scaffold degradation need to be fine-tuned. Methods We recently described the co-culture of adipose-derived stem cells with endothelial cells in fibrin, resulting in capillary formation in vitro as well as their perfusion in vivo. Here, we aimed to further characterise microvascular tube formation in fibrin by determining the role of scaffold degradation, thrombin concentration and culture conditions on vascularisation. Results We observed that inhibition of cell-mediated fibrin degradation by the commonly used inhibitor aprotinin resulted in impaired vascular network formation. Aprotinin had no effect on laminin and collagen type IV deposition or formation of tube-like structures in scaffold-free co-culture, indicating that poor vascularisation of fibrin clots is primarily caused by inhibition of plasminogen-driven fibrinolysis. Co-culture in plasminogen- and factor XIII-depleted fibrin did not result in different vascular network density compared to controls. Furthermore, we demonstrate that thrombin negatively affects vascular network density at high concentrations. However, only transient activation of incorporated endothelial cells by thrombin could be observed, thus excluding a long-term inflammatory response in tissue-engineered micro-capillaries. Finally, we show that vascularisation of fibrin scaffolds in basal medium is undermined because of increased fibrinolytic activity leading to scaffold destabilisation without aprotinin. Conclusions Taken together, our data reveal a critical role of fibrinolysis inhibition in in vitro cell-mediated vascularisation of fibrin scaffolds

    Additional file 4: of The role of fibrinolysis inhibition in engineered vascular networks derived from endothelial cells and adipose-derived stem cells

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    Aprotinin does not impair network formation of endothelial cells in a 2D setup. CD31 staining of endothelial cells (either HUVEC or ECFC) reveals tube-like structures when co-cultured with ASC in a 2D-setup devoid of fibrin. Aprotinin (100 KIU/ml) has no effect on HUVEC 2D tube formation. Scale bar: 100 μm or 50 μm as indicated. (DOC 370 kb

    Additional file 5: of The role of fibrinolysis inhibition in engineered vascular networks derived from endothelial cells and adipose-derived stem cells

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    Thrombin transiently activates EC. HUVEC-ASC clots were fixed and immunofluorescence was performed against E-Selectin after 4┬áh, 1┬áday, 4┬ádays and 7┬ádays. Staining revealed transient activation of EC via thrombin after 4┬áh. TNF-╬▒ (10┬áng/ml), serving as a positive control, activated EC more strongly after 4┬áh than thrombin alone. No aprotinin was used in any sample. nÔÇë=ÔÇë8 from two independent experiments. Scale bar: 200┬á╬╝m. (DOC 322 kb
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