4 research outputs found

    Indirect immunofluorescence microscopy (A,B,C&D).

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    <p>Anti-Msp1 (A&B) and anti-Msp2 (C&D) rabbit antisera were used on wild-type (A&C) en <i>msp1</i> mutant cells (B&D). Anti-rabbit IgG antibodies conjugated with Alexa Fluor 488 (Invitrogen) were used to visualize the Msp1 and Msp2 localization on the cells.</p

    Main muropeptides from LGG PG digested with or without Msp1.

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    a<p>Peak numbers refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031588#pone-0031588-g005" target="_blank">Figure 5</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031588#pone.0031588.s001" target="_blank">Figure S1</a>.</p>b<p>Di, disaccharide dipeptide (L-Ala-D-iGln); Tri, disaccharide tripeptide (L-Ala-D-iGln-L-Lys); Tetra, disaccharide tetrapeptide (L-Ala-D-iGln-L-Lys-D-Ala); Disaccharide, GlcNAc-MurNAc; Ac, acetylation, iGln, isoglutamine; N, D-Asn; A, D-Ala; K, L-Lys.</p>c<p>Sodiated molecular ions were the most abundant ones on MALDI-TOF mass spectra for all muropeptides.</p>d<p>Percentage of each peak was calculated as the ratio of the peak area over the sum of areas of all the peaks identified in the corresponding chromatogram (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031588#pone-0031588-g005" target="_blank">Figure 5</a>).</p>e<p>ND, non detected.</p

    RP-HPLC separation profile of muropeptides from LGG digested with mutanolysin (A) and digested with mutanolysin and Msp1 (B).

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    <p>Peak numbers refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031588#pone-0031588-t001" target="_blank">Table 1</a>. DS-di, disaccharide dipeptide (GlcNAc-MurNAc-L-Ala-D-Gln). The schematic structure of LGG peptidoglycan and site of cleavage of Msp1 is also represented. GlcNAc, N-acetylglucosamine; MurNAc, N-acetylmuramic acid.</p