35 research outputs found

    Tratamiento anticoagulante con Heparina IV a dosis terapéuticas y hemorragia retroperitoneal

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    Se presenta un caso clínico de un paciente tratado con heparina intravenosa a dosis terapéuticas por un cuadro compatible con tromboembolismo pulmo­nar y muerte por shock hipovolémico debida a he­morragia retroperitoneal

    Gallbladder dyskinesia in acalculous biliary colic

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    Objetivos: probar las hip√≥tesis de que: 1) el vaciamiento postprandial de la ves√≠cula biliar puede estar alterado en pacientes con c√≥lico biliar aliti√°sico; y que 2) la himecromona puede mejorar los s√≠ntomas. M√©todos: se estudiaron 12 pacientes que presentaban c√≥lico biliar sin colelitiasis. y contraste en la ves√≠cula biliar 24 horas despu√©s de una colecistograf√≠a oral. Sus resultados se compararon a los de doce voluntarias sanas que no presentaban s√≠ntomas. y que no reten√≠an contraste tras la colecistograf√≠a oral. El vaciado vesicular se estudi√≥ mediante ecograf√≠a en ambos grupos. antes y despu√©s de la administraci√≥n oral de himecromona. Resultados: El volumen de la ves√≠cula biliar fue parecido en ambos grupos (23 ¬Ī 10 mi vs. 31 ¬Ī 11 mi; NS). El vaciado vesicular en el grupo control fue de tipo exponencial (R2 = 0.948 ¬Ī 0.059) con una constante de 32 ¬Ī 16 minutos. El vaciado vesicular de las pacientes fue bimodal. La ves√≠cula aument√≥ de volumen hasta un 120% en los primeros 5 minutos post-prandiales, para vaciarse de forma exponencial despu√©s (R2 = 0,964 ¬Ī 0.040. Constante: 29 ¬Ī 22 minutos). Este hecho determin√≥ una reducci√≥n en el volumen vesicular vaciado a los 40 minutos en comparaci√≥n al grupo control (30% ¬Ī 27% pacientes vs. 54% ¬Ī 14% control p = 0.012). El pretratamiento de los controles con himecromona result√≥ en un patr√≥n de vaciado parecido al de las pacientes. En las pacientes, la himecromona no modific√≥ el vaciado pero los s√≠ntomas mejoraron . Conclusiones: se identific√≥ un patr√≥n de vaciado vesicular anormal en las pacientes con c√≥lico biliar aliti√°sico. La himecromona fue parcialmente eficaz en el alivio del dolor. a pesar de un efecto m√≠nimo sobre el vaciado vesicular

    An√°lisis ecol√≥gico de la culturizaci√≥n del paisaje de alta monta√Īa desde el neol√≠tico : los parques nacionales de monta√Īa como modelo

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    Ajuts: Agradecemos al Organismo Aut√≥nomo Parques Nacionales, la financiaci√≥n del proyecto 998/2013 y las facilidades que en √©ste y otros estudios anteriores - base para el mismo- han brindado los gestores de Parque Nacional de Sierra Nevada, Parque Nacional de Aig√ľestortes i Estany de San Maurici, Parque Nacional de Ordesa y Monte Perdido y Parque Nacional de Picos de Europa.La presencia inicial de los humanos en la alta monta√Īa desde hace poco menos de 8000 a√Īos presenta m√°s ele-mentos de domesticaci√≥n de lo que inicialmente se hab√≠a estimado. Aunque la extensi√≥n geogr√°fica de las explo-raciones arqueol√≥gicas es todav√≠a limitada, hay evidencias de uso de ganado dom√©stico y, en algunos casos, unaprobable agricultura utilizando mezcla de cereales a cotas intermedias. Por el momento, no se ha podido constatarimpacto en el paisaje de esta actividad. En los Pirineos, hay una variaci√≥n en la distribuci√≥n de yacimientos ar-queol√≥gicos en la alta monta√Īa asociada con el cambio clim√°tico de mitad del Holoceno. Decrece la ocupaci√≥n deabrigos y el n√ļmero de yacimientos en su conjunto. Sin embargo, el impacto humano sobre el paisaje es evidentedurante la Edad del Bronce y supone la apertura de espacios para el pastoreo, probablemente, en muchas ocasio-nes utilizando fuego. La Edad Media supone la transformaci√≥n definitiva del paisaje y una organizaci√≥n del usodel territorio que se ha mantenido en buena medida hasta la actualidad. Al uso ganadero se a√Īade una mayorvariedad de explotaciones, como la miner√≠a y metalurgia, que inciden tambi√©n en una fuerte presi√≥n sobre los bos-ques. En Sierra Nevada, el desarrollo del sistema de acequias por los andalus√≠es condiciona la hidrolog√≠a hasta laactualidad, seguramente con otras implicaciones sobre el paisaje todav√≠a por especificar. Es probable que la huellamedieval est√© todav√≠a presente en la distribuci√≥n actual de las especies y en los procesos ed√°ficos que se dan en laalta monta√Īa. La proyecci√≥n espacial y continuidad temporal sobre c√≥mo se dio la culturizaci√≥n del paisaje de laalta monta√Īa, desde el mosaico inicial de incipientes transformaciones hasta la contundencia y diversidad de laEdad Media, todav√≠a requiere de una exploraci√≥n m√°s profunda a lo largo del territorio.The initial presence of humans in the high mountains almost since 8000 years ago shows more elements ofdomestication than initially estimated. Although the geographic extent of archaeological explorations is stilllimited, there is evidence of domestic livestock use and, in some cases, likely mixed-cereal agriculture at in-termediate altitudes. It has not been possible to verify an impact of this activity on the landscape yet. In thePyrenees, the distribution of archaeological sites in the high mountain changes associated with climate in themiddle of the Holocene. The occupation of shelters and the number of archaeological sites decline. However,the human impact on the landscape is evident in some palaeoecological registers during the Bronze Age andinvolves the forest opening for grazing, probably using fire, on many occasions. The Middle Ages constitute thedefinitive transformation of the landscape and an organization of the use of the territory that has remained lar-gely to present. A greater variety of land use, such as mining and metallurgy, added to livestock also with strongimpact on forests. In the Sierra Nevada, the development of the irrigation ditch system by the Andalusiansmodified the hydrology to the present, with other implications on the landscape yet to be determined. Likely,the medieval imprint is still present in the current distribution of the species and the edaphic processes that oc-cur in the high mountain. From the initial mosaic of incipient transformations to the forcefulness and diversityof the Middle Ages, the spatial projection and temporal continuity on how the landscape acculturation of thehigh-mountain took place still require a deeper exploration throughout the territory

    miRNA let-7e targeting MMP9 is involved in adipose-derived stem cell differentiation toward epithelia

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    miRNA let-7e is involved in stem cell differentiation, and metalloproteinases are among its potential target genes. We hypothesized that the inhibitory action of let-7e on regulation of MMP9 expression could represent a crucial mechanism during differentiation of adipose-derived stem cells (ASCs). ASCs were differentiated with all-trans retinoic acid (ATRA) to promote differentiation, and the effect of let-7 silencing during differentiation was tested. Results indicate that ASCs cultured with ATRA differentiated into cells of the epithelial lineage. We found that ASCs cultured with ATRA or transfected with miRNA let-7e expressed epithelial markers such as cytokeratin-18 and early renal organogenesis markers such as Pax2, Wt1, Wnt4 and megalin. Conversely, the specific knockdown of miRNA let-7e in ASCs significantly decreased the expression of these genes, indicating its vital role during the differentiation process. Using luciferase reporter assays, we also showed that MMP9 is a direct target of miRNA let-7e. Thus, our results suggest that miRNA let-7e acts as a matrix metalloproteinase-9 (MMP9) inhibitor and differentiation inducer in ASCs

    miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation

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    This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCő≤, GSK3ő≤ phosphorylation (GSK3ő≤(P)) and ő≤-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCő≤ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3ő≤(P) and ő≤-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3ő≤ phosphorylation and ő≤-catenin production

    Leiomiosarcoma en el recte

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    Els tumors de parts blanes comprenen una gran varietat de tumors malignes que s'agrupen per llur similitud clínica, anatomopatológica i evolutiva. Es caracteritzen perquè la majoria deriven de teixit conjuntiu, musculatura llisa i d'altres estructures mesodèrmiques

    Nucleotides modulate renal ischaemia-reperfusion injury by different effects on nitric oxide and superoxide

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    1. The present study investigated the effects of kidney ischaemia duration on nitric oxide (NO) and superoxide (O2 -) generation at reperfusion and the role of xanthine and adenosine as mediators of NO/O2 - generation. 2. The effect of the duration of ischaemia on renal nucleotide levels was studied in two ischaemic groups (10 and 30 min). The role of adenosine and xanthine was studied in ischaemic-reperfused groups (subjected to 10 and 30 min ischaemia and 60 min reperfusion). 3. Tissue levels of adenosine decreased significantly after 30 min ischaemia, whereas xanthine/hypoxanthine levels increased concomitantly with renal dysfunction and histological damage. 4. Nitric oxide production increased significantly after 10 min ischaemia and 60 min reperfusion, whereas lipoperoxidation increased significantly after 30 min ischaemia and 60 min reperfusion. The administration of theophylline (40 mg/kg, i.p.) reversed the early increase in NO production. 5. Xanthine supplementation decreased renal function and increased lipoperoxidation. 6. In conclusion, NO/O2 - production and the subsequent renal injury/dysfunction may be modified by changes in the adenosine and xanthine levels of the injured kidney, although the present data show a significant in vivo role only for xanthine.This study was supported by the following grants: EC grant QLK6-2000-00064, FISS 01/1691 and SAF2000-3090-CE (Ministerio de Ciencia y Tecnología)Peer Reviewe

    Role of changes in tissular nucleotides on the development of apoptosis during ischemia/reperfusion in rat small bowel

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    The aim of this study was to evaluate whether xanthine and adenosine, substances modified proportionally to the duration of ischemia, can determine cell demise (apoptosis/necrosis) during intestinal ischemia/reperfusion (I/R) and to determine the role of nitric oxide (NO) during this process. The following experimental groups were studied: I, cold ischemia; I+X, effect of xanthine; I+T, effect of adenosine (blocking its receptor by theophylline); I+A, effect of excess adenosine; I+T+X, effect of xanthine alone, and I+T+ spermine NONOate (NONOs), I+A+NONOs, I+X+NONOs, role of NO. DNA fragmentation, xanthine/adenosine levels, caspase-3 activity, NO generation, and histological analysis were measured in tissue samples. The rats treated with xanthine or adenosine showed increased levels of caspase-3 activity and DNA fragmentation. In contrast, theophylline-treated rats showed decreased levels of DNA fragmentation and tended to show lower mean values of caspase-3 activity. Administration of xanthine or NONOs to theophylline-treated rats reversed these effects. The results of histological evaluation were in agreement with these previous results. In conclusion, the present study indicates that xanthine and adenosine induced an apoptotic response during cold ischemic preservation of rat small intestine. In particular, the action of adenosine on apoptotic events was mediated by NO. We consider that identification of the role of these factors may help to define the best conditions of tissue preservation before intestinal transplantation.Supported by FIS Fondo de Investigacio¬īn Santitaria 01/1691Peer Reviewe

    Nitric oxide enhances endothelin production in pancreas transplantation

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    The role of endothelin and its relationship with nitric oxide (NO) production in ischemia-reperfusion associated with pancreas transplantation has been explored. For this purpose, pancreatic levels of endothelin were evaluated in an experimental model of pancreas transplantation after different periods of cold preservation. The effects of NO synthase inhibition were also evaluated. Results show posttransplantation increases in lipase and endothelin production. The release of lipase and endothelin was only prevented by N(G)-nitro-L-arginine methyl ester after a short ischemic period. Thus, endothelin synthesis could be a consequence of stimulation with NO in the ischemia-reperfusion associated with pancreas transplantation.Peer Reviewe
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