24 research outputs found

    Fibroblasts on RADA16 (1% wt)

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    Live cell tracking migration assay with fibroblasts on RADA16 (1% wt)

    Data from: Designer self-assembling hydrogel scaffolds can impact skin cell proliferation and migration

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    There is a need to develop economical, efficient and widely available therapeutic approaches to enhance the rate of skin wound healing. The optimal outcome of wound healing is restoration to the pre-wound quality of health. In this study we investigate the cellular response to biological stimuli using functionalized nanofibers from the self-assembling peptide, RADA16. We demonstrate that adding different functional motifs to the RADA16 base peptide can influence the rate of proliferation and migration of keratinocytes and dermal fibroblasts. Relative to unmodified RADA16; the Collagen I motif significantly promotes cell migration, and reduces proliferation

    Macromolecular approach for targeted radioimmunotherapy in non-Hodgkin's lymphoma

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    Polymers are an attractive anchoring platform for the synthesis of radioimmunoconjugates. They enable independent control over the amount of radioisotope loading and antibody attachment, which is pivotal in developing tailorable formulations for personalised medicine. Herein, we report the synthesis of p(HEMA-ran-GMA) for the conjugation of lutetium ions and rituximab as a functional platform for radioimmunotherapy. We demonstrate the suitability of this platform using non-Hodgkin's lymphoma cells

    Colloidal Polymeric Platform for Facile Click-Assisted Ligand Functionalization and Receptor Targeting

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    Colloidal poly­(glycidyl methacrylate) nanoparticles (NPs) are demonstrated to be platforms facilitating the “click” chemistry approach of surface functionalization for receptor targeting. Folate receptor-targeted NPs were synthesized, physicochemically characterized, confirmed for their biocompatibility, and validated for their selective targeting capabilities for ovarian cancer cells in vitro

    Seasonal and diurnal variation of PM2.5 HULIS over Xi'an in Northwest China: Optical properties, chemical functional group, and relationship with reactive oxygen species (ROS)

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    Humic-like substances (HULIS) in particulate matter (PM) play critical roles in the atmospheric changes in our environment. In this study, high time resolution PM2.5 samples were collected to insight the abundances, spectroscopic characters, chemical groups, and oxidative potential of HULIS in Xi'an, China. The average mass concentrations of HULIS in term of carbon (HULIS-C) was 11.55 +/- 5.85 and 8.28 +/- 2.23 mu g C m(-3) in winter and summer, respectively. The diurnal variations of HULIS displayed three peaks (03:00-07:00, 10:00-14:00, and 18:00-21:00 LT) in winter, but a single peak (08:00-12:00 LT) in summer. The optical parameters show obvious difference between winter and summer. Fourier Transform infrared (FT-IR) spectra revealed that the HULIS mainly consisted of aliphatic chains, aromatic rings, and carboxylic groups. The FT-IR spectra proved that the aromatic compounds and carboxylic acids dominated the diurnal variation in winter, while carboxylic acids were responsible for that in summer. The normalized oxidative potential of HULIS exhibited an inverse trend with the HULIS-C concentration measured by the DCFH assay. The diurnal reactive oxygen species (ROS) productions from HULIS presents three valleys (03:00-07:00, 10:00-14:00, and 18:00-21:00 LT) in winter but only a single valley (08:00-12:00 LT) in summer. In addition, a positive correlation (R-2 approximate to 0.6, p<0.01) between the oxidative potential and specific ultraviolet absorbance at a wavelength of 254 nm (SUVA254) in winter suggested that the oxidative power of HULIS could be more related to the compounds with high light absorbance, high aromaticity,and molecular weight. The results of this study offer more solid knowledge on the spectral and chemical char- acteristics and oxidative potential of the HULIS in PM2.5 of the typical city in northwest China

    Oxidative stress-inducing effects of various urban PM2.5 road dust on human lung epithelial cells among 10 Chinese megacities

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    PM2.5 Road dust samples were collected from 10 representative cities in southern and northern China for examination of chemical components and oxidative stress levels in A549 cells. Downtown road dust was abundance of heavy metals, EC and PAHs compared to nondowntown road dust. Source apportionment also revealed the relative higher contribution of vehicle emission to downtown (35.8%) than nondowntown road dust (25.5%). Consequently, downtown road dust induced much higher intracellular reactive oxidative species (ROS) levels than that from nondowntown (p < 0.05). This study highlights that the ROS-inducing capacity of road dust in China is lower at lower latitudes, which resulted in a significantly higher ROS-inducing capacity of road dust from northern cities than southern ones. Hotspot analysis demonstrated that heavy metals (i.e., Cr, Zn, Cu and Pb) in road dust were the most closely associated with ROS production in A549 cells. Vehicle emission and combustion emission in road dust were identified to be correlated with cellular ROS production. The findings highlight the ROS-inducing effect of PM2.5 road dust and also serve as a reference to make the targeted solutions for urban road dust pollution control, especially from a public health perspective
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