Similarity Breeds Proximity: Pattern Similarity within and across Contexts Is Related to Later Mnemonic Judgments of Temporal Proximity

SummaryExperiences unfold over time, but little is known about the mechanisms that support the formation of coherent episodic memories for temporally extended events. Recent work in animals has provided evidence for signals in hippocampus that could link events across temporal gaps; however, it is unknown whether and how such signals might be related to later memory for temporal information in humans. We measured patterns of fMRI BOLD activity as people encoded items that were separated in time and manipulated the presence of shared or distinct context across items. We found that hippocampal pattern similarity in the BOLD response across trials predicted later temporal memory decisions when context changed. By contrast, pattern similarity in lateral occipital cortex was related to memory only when context remained stable. These data provide evidence in humans that representational stability in hippocampus across time may be a mechanism for temporal memory organization

Lagrangian formalism and Lie group approach for commutative semigroup of differential equations

A set of linear second-order differential equations is converted into a semigroup, whose algebraic structure is used to generate many novel equations. Two independent methods that can be used to derive the equations of the semigroup are considered, namely, the Lagrangian formalism and the Lie group approach. The advantages and disadvantages of each method are discussed, and it is shown that the Lagrangian formalism can be established for all equations of the semigroup, however, the Lie group approach is only limited to a certain sub-semigroup . The obtained results are discussed in the context of their applications in mathematical physics.Comment: 17 page

Therapeutic efficacy of perfloxacin in treatment of ampicillin-resistant typhoid fever in 7 days versus 10 days

AbstractObjective: Typhoid fever is endemic in Albania and is becoming increasingly resistant to ampicillin, chloramphenicol, and trimethoprim-sulfamethoxazole. Perfloxacin has been shown to have significant activity in vitro against Salmonella species. the authors studied its therapeutic efficacy in patients with typhoid fever.Methods: Thirty patients with ampicillin-resistant typhoid fever, who were admitted to the Infectious Disease Clinic at the University Hospital Center of Tirana, were enrolled in this prospective, nonblinded clinical trial. Patients were treated with oral perfloxacin at 400 mg twice daily. They were randomized to receive treatment for either 7 days (group I) or 10 days (group II).Conclusions: Excellent therapeutic responses were observed in all patients in both groups. In every case, there was significant clinical improvement with defervescence and sterile blood cultures by day 4 and three consecutive negative urine and stool cultures at the end of treatment. Perfloxacin was highly effective in treatment of typhoid fever. Treatment for 7 days appeared to be as effective as treatment for 10 days. In both groups, bile cultures at the end of treatment and at 2 months follow-up were sterile, suggesting that both regimens were effective in preventing a chronic carrier state

They saw a movie: Long-term memory for an extended audiovisual narrative.

We measured long-term memory for a narrative film. During the study session, participants watched a 27-min movie episode, without instructions to remember it. During the test session, administered at a delay ranging from 3 h to 9 mo after the study session, long-term memory for the movie was probed using a computerized questionnaire that assessed cued recall, recognition, and metamemory of movie events sampled ∼20 sec apart. The performance of each group of participants was measured at a single time point only. The participants remembered many events in the movie even months after watching it. Analysis of performance, using multiple measures, indicates differences between recent (weeks) and remote (months) memory. While high-confidence recognition performance was a reliable index of memory throughout the measured time span, cued recall accuracy was higher for relatively recent information. Analysis of different content elements in the movie revealed differential memory performance profiles according to time since encoding. We also used the data to propose lower limits on the capacity of long-term memory. This experimental paradigm is useful not only for the analysis of behavioral performance that results from encoding episodes in a continuous real-life-like situation, but is also suitable for studying brain substrates and processes of real-life memory using functional brain imaging. Experimental protocols that probe brain correlates of episodic memory formation commonly use paradigms in which memoranda are presented as individual items devoid of continuous context outside of the laboratory setting Movies are capable of simulating aspects of real-life experiences by fusing multimodal perception with emotional and cognitive overtones Here, we describe the use of a 27-min narrative movie to investigate long-term cued recall and recognition as well as metamemory judgments. We measured memory performance of several groups of participants, each at a different delay ranging from 3 h to 9 mo after watching the movie, by probing memory for events occurring in the movie ∼20 sec apart. Participants remembered many events in the movie that they had seen only once without prior instructions to remember it, even months after watching it. We have dissected multiple facets of memory and metamemory as a function of time and type of occurrence in the movie. Our analysis also suggests lower limits on the capacity of long-term memory for a real-life-like situation. Results Memory of the movie persists for months The first set of experimental groups watched the movie and answered the questionnaire ( Heuristic subdivisions in long-term memory Pairwise comparisons among all groups, corrected for multiple comparisons, reveal superior performance of the 3-h, 1-wk, and 3-wk groups compared with the 3-mo and 9-mo groups ( Recall attempts over time Participants made fewer attempts at recall as more time passed between study and test sessions ( Memory confidence over time The proportions of all answers made at different levels of confidence were compared across time-interval groups, revealing a surprisingly stable proportion of high-confidence recognition (HCR) answers over time, and a significant decrease in the use of recall over time. In contrast, the proportion of low-confidence recognition (LCR) and guess answers significantly increased over time ( One might expect a smaller proportion of correct answers as confidence declines with the passage of time. To test this assumption, the proportion of correct answers was calculated from all answers made in each level of confidence Furthermore, the main group effect suggests that distribution of correct answers between the four possible levels of confidence is uneven among time-interval groups. The interaction between these two main effects, time and confidence-level, was further explored by performing pairwise contrasts, revealing performance differences that further support differentiation of ST and LT groups. As can be seen in The findings described above indicate that confidence measures of memory are time-sensitive: more high-confidence answers (using recall and HCR) are used after short time durations, while more lower-confidence answers (LCR and guessing) are used after longer time durations. We further sought to characterize the temporal dynamics of metamemory measures using analysis of answers by confidence level. We find that on the one hand, proportion of overall use of recall declines over time Memory density The availability of memory performance scores that sample events every 20 sec in a 27-min episode renders it tempting to attempt tapping into long-term memory capacity per unit time, or density (Dudai 1997). Any such attempt is bound to yield only rough estimates. First, formal units that might be used for quantifying the stored memory, such as bits Using the algorithm specified in the Materials and Methods, we estimate that 56% of information in the movie is remembered after 3 h, while 53%, 39%, 25%, and 19% are remembered after 1 wk, 3 wk, 3 mo, and 9 mo, respectively. Equating for the sake of calculation a memory unit as a questionnaire item to be answered correctly and assuming independence among items, Long-term memory of a movie Learning & Memory 459 www.learnmem.org Cold Spring Harbor Laboratory Press on December 7, 2007 -Published by www.learnmem.org Downloaded from hence, 77 items that could be potentially encoded over 27 min, this implies retrievability ranging from 1.6 items per minute movie after 3 h to 1.4 and 1.0 items per 2 min after 3 and 9 mo, respectively. The robustness of the assumptions involved and the relevance to previous estimates of long-term memory capacity are discussed below. Recall and recognition as function of memory content Six independent raters were asked to classify the questions into eight predetermined categories (while allowing overlap of categories). Questions were then grouped into nonoverlapping clusters based on 66% agreement among raters. Clusters included plot themes, social interactions, couple relationship, and jokes and minor details (13, 19, 6, 5, and 14 questions, respectively). "Social interactions" is a nonoverlapping broader category than "couple relationship," and did not include questions relating to interactions between the central character and his partner, which were included in the "couple relationship" cluster. Twenty of 77 items in the memory questionnaire were not entered into analysis, as the agreement criterion was not reached. For each content cluster, we examined proportion of recall and recognition attempts We find that narrative elements and social interactions between characters are remembered best ("plot themes" questions elicited close to 100% accuracy in all ST groups). Questions about "jokes" and "details" elicit less recall attempts and answer accuracy declines more rapidly and drastically than for all other question clusters. Better performance of ST groups relative to LT groups is established for all content clusters, while comparison of time-dependent performance decline (i.e., curve slope) between content clusters approached significance only for correct recall performance. Significant main effects of content and time-interval group were found (recall attempts: content F (4,3224) = 62.27, P < 0 . 0 0 0 1 , t i m e -i n t e r v a l g r o u p F (4,44.13) = 4.96, P < 0.002; correct recall: content F (4,874) = 8.02, P < 0.0001; timei n t e r v a l g r o u p F ( 4 , 7 5 . 5 4 ) = 8 . 4 9 , P < 0.0001). The interaction between these effects (difference in performance between content clusters as a function of time-interval group, or comparison of slopes) was insignificant for both analyses, but approached significance for correct recall (recall attempts: F (16,3224) = 1.1, P < 0.35; correct recall: F (16,874) = 1.52, P < 0.09). In order to further explore main effects, pairwise contrasts were performed separately according to time-interval groups It is noteworthy that the division of LTM performance into ST and LT groups, introduced above on the basis of analysis of correct and incorrect answers, is also supported by the analysis of content-based correct recall answers, and is partially supported by analysis of content-based recall attempts. Subjects in the ST groups made significantly more recall attempts and more correct recall answers, per content cluster, than subjects in the LT groups. (Two exceptions are the "couple relationship" question cluster, which elicited a high proportion of correct recall answers throughout the tested span, and proportion of recall attempts of the 3-wk group that did not differ from both LT groups; Tables 1, 3.) While superior performance across content clusters is found Cold Spring Harbor Laboratory Press on December 7, 2007 -Published by www.learnmem.org Downloaded from for the ST groups, different content clusters elicited significantly different performance profiles over time. This is illustrated by observing that the starting point of maximal value for forgetting curves varies between plots of different content clusters We should qualify our finding that memory performance can be differentiated according to content elements, as it seems that time since encoding is also an important factor in rendering this differentiation evident. Further examination of correct recall data, where interaction between content and group effects was found to only approach significance, was performed using post hoc comparisons. When examining content effects within timeinterval groups, significant differences between content clusters were found in all but the 3-h and 1-wk groups Manipulation of movie and questionnaire did not diminish memory We further tested whether certain manipulations of the movie during the study session, or of the questionnaire in the test session, or both, will affect memory performance. A separate set of experimental groups all participated in the test session 3 wk after watching the movie, but were subjected to manipulated study or test material. Manipulations were either in the order of content material (scrambling the order of scenes in the movie or the questions in the test) or in perceptual attributes (eliminating color from the movie). One experimental group performed an interference protocol, in which subjects watched a different episode from the same sitcom at the beginning of the test session, and immediately afterward completed the original computerized questionnaire. No significant differences were found in performance (correct answers, collapsing confidence levels) between manipulation protocols and the original 3-wk group (unaltered movie and test, n = 8, 78.79 ‫ע‬ 2.02% correct; scrambled version of the movie followed by regular test, n = 10, 79.87 ‫ע‬ 2.38% correct; unaltered movie followed by scrambled test, n = 6, 73.81 ‫ע‬ 2.97% correct; scrambled movie followed by scrambled test, n = 7, 73.1 ‫ע‬ 2.76% correct; interference protocol, n = 7, 73.28 ‫ע‬ 2.39% correct; regular movie in black and white and regular test using black and white frames as visual cues, n = 6, 70.35 ‫ע‬ 2.99% correct; F (5,40.25) = 2.24, P < 0.07). It is noteworthy that although some of the manipulations did show a trend for decreased performance, scrambling of the order of the scenes in the movie itself had no effect whatsoever. The potential implication of this finding to the encoding of the study material is discussed below. Discussion We describe a memory paradigm in which the study material is a 27-min narrative movie. This paradigm was intended to mimic aspects of "real-life" learning and memory under controlled experimental settings. We tested the memory once, in delays ranging from 3 h to 9 mo after the study session. The test targeted events that occur in the movie every ∼20 sec. We found that details from the movie, which the participants watched only once without prior instruction to remember it, were remembered well over several months. Multiple performance measures indicate that long-term memory after hours-to-weeks is different from memory performance after several months. Recall answers, which we considered as the highest confidence answers, proved to be reliable measures of memory only for shorter durations, while HCR answers were highly reliable throughout the measured time span. Despite manipulation of movie and test materials, meant to disrupt narrative construction during encoding and/or retrieval, memory performance was unaffected. One possible explanation is that subjects were still able to successfully reconstruct the narrative from the scrambled segments. We further demonstrate that information content significantly influences memorability over time (though some time is needed for this effect to become evident). Finally, we use the unique resolution of our memory questionnaire to suggest that memory capacity for real-life information might be higher than previously estimated. In clinical neurology and in cellular neurobiology, memory after 3 h is already long-term memory, and memory after 9 mo could be considered remote memory. Studies of human longterm memory and remote memory are abundant in the literature (for review, se

Effect of oral administration of pioglitazone on follicular dynamics in Holstein dairy cows

This study investigated the effects of oral administration of pioglitazone (PGT), a specific and synthetic ligand of peroxisome proliferator-activated receptors gamma (PPARγ), on follicular dynamics and corpus luteum (CL) functionality in dairy cows. Cows exhibiting strong signs of estrus after 2 injections of PGF2α (given14 d apart) at d 30 postpartum (n = 28) were allotted to four groups (n = 7 cows/treatment) and orally received 6 mg PGT/kg body weight/day according to the following protocol: no PGT (control); PGT for 14 d from 7 d before expected estrus (10 d after 1st injection of PGF2α) to 7 d after observed estrus (PGT14); PGT for 21 d after observed estrus (PGT21); and PGT for 28 d, 7 d before expected estrus to 21 d after observed estrus (PGT28). During the first follicular wave, number of follicles (total and small) increased in PGT14 and PGT28 cows compared to the control group (P < 0.05). During the ovulatory wave, number of total and small follicles increased in PGT28 (P < 0.05) and PGT21 (P < 0.10) compared with PGT14 and control cows. Size of the largest follicle at first wave was greater in PGT28 (P < 0.05), PGT14 (P < 0.05) and PGT21 (P < 0.10) compared to the control cows. Maximal size of the ovulatory follicle was greater in PGT28 (P < 0.05) and PGT21 (P < 0.10) groups compared to the control group. Growth rate of the largest follicle at first wave was higher (P < 0.05) in PGT-treated cows, while growth rate of the ovulatory wave was higher in PGT28 and PGT21 groups, leading to shorter days from luteolysis to ovulation. Pioglitazone administration did not affect CL size, but increased progesterone (P4) concentration. The PGT14 and PGT28 cows had higher maximal plasma P4 concentration and shorter intervals to reach maximal plasma P4 compared to the control group. In conclusion, oral administration of PGT had some positive effects on follicular development and circulating P4 levels which may be conducive to better reproductive performance