11 research outputs found

    Monitoring pollution of coastal lagoon using Liza aurata kidney oxidative stress and genetic endpoints: an integrated biomarker approach

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    Despite the importance of fish kidney in several functions (immune, metabolism and excretion of xenobiotics) its use in coastal water biomonitoring focusing on protection and damage is scarce. Five critical sites in Ria de Aveiro (Portugal; Barra‚ÄĒBAR, Gafanha‚ÄĒGAF, Rio Novo do Principe‚ÄĒRIO; Laranjo‚ÄĒLAR and Vagos‚ÄĒ VAG) were assessed in comparison to a reference site (Torreira‚ÄĒTOR), focusing on Liza aurata kidney antioxidant defences versus damage responses. Non protein thiols were higher at RIO (near a former bleached kraft pulp mill effluent) and total glutathione at RIO, LAR (mercury contaminated) and VAG (polycyclic aromatic hydrocarbons contaminated). Catalase and glutathione S-transferase activities were higher at RIO and LAR whereas no differences were found in glutathione peroxidase activity. However, glutathione reductase was higher at BAR (subject to naval traffic), GAF (harbour water area), RIO and LAR. No peroxidative damage was observed despite the decreased DNA integrity at RIO and VAG. The integrated biomarker response index ranked impacted sites as: LAR>RIO>BAR>GAF>VAG>TOR

    Integrated assessment of water quality of the Costa da Morte (Galicia, NW Spain) by means of mussel chemical, biochemical and physiological parameters

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    Glutathione and its dependent enzymes' modulatory responses to toxic metals and metalloids in fish: a review

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    Toxic metals and metalloid are being rapidly added from multiple pathways to aquatic ecosystem and causing severe threats to inhabiting fauna including fish. Being common in all the type of aquatic ecosystems such as freshwater, marine and brackish water fish are the first to get prone to toxic metals and metalloids. In addition to a number of physiological/biochemical alterations, toxic metals and metalloids cause enhanced generation of varied reactive oxygen species (ROS) ultimately leading to a situ- ation called oxidative stress. However, as an important com- ponent of antioxidant defence system in fish, the tripeptide glutathione (GSH) directly or indirectly regulates the scav- enging of ROS and their reaction products. Additionally, several other GSH-associated enzymes such as GSH reduc- tase (GR, EC 1.6.4.2), GSH peroxidase (EC 1.11.1.9), and GSH sulfotransferase (glutathione-S-transferase (GST), EC 2.5.1.18) cumulatively protect fish against ROS and their reaction products accrued anomalies under toxic metals and metalloids stress conditions. The current review highlights recent research findings on the modulation of GSH, its redox couple (reduced glutathione/oxidised glutathione), and other GSH-related enzymes (GR, glutathione peroxidase, GST) involved in the detoxification of harmful ROS and their reaction products in toxic metals and metalloids-exposed fish
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