3,181 research outputs found

    Au@MNPs-based electrochemical immunosensor for vitamin D3 serum samples analysis

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    We report a new sensitive label-free electrochemical immunosensor to detect Vitamin D3 (25-OHD3) in untreated serum samples. To this aim, a graphite screen printed electrode (SPE) was modified using cysteamine (CYM) functionalized core-shell magnetic nanoparticles (Au@MNPs) then, the 25-OHD3 antibody (AbD) was immobilized via glutaraldehyde crosslinking. The several steps involved in the immunosensor development and 25-OHD3 analysis were monitored by using differential pulse voltammetry (DPV). The developed immunosensor showed a LOD of 2.4 ng mL‚ąí1 and a linear range between 7.4 and 70 ng mL‚ąí1. The effectiveness of the immunosensor in human serum analysis was assessed by comparing the results obtained with the chemiluminescence-immunoassay (CLIA) reference method. The high sensitivity and excellent agreement with the reference method suggest its potential use as a POCT to monitor hypovitaminosis 25-OHD levels

    Stoma-free Survival After Rectal Cancer Resection With Anastomotic Leakage: Development and Validation of a Prediction Model in a Large International Cohort.

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    Power plant design for all-electric ships considering the assessment of carbon intensity indicator

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    The design and management of a ship power system determine the amount of CO2 emitted during the ship operations. Recently, the International Maritime Organization has introduced the Carbon Intensity Indicator to limit the environmental impact of ships, pushing designers to define new solutions to reduce polluting emissions. The proposed methodology allows to estimate the environmental performances of a ship during the preliminary design phase, starting from the mission analysis. The tool simulates different power plant configurations, including diesel generators with different type of fuels, battery energy storage systems, fuel cells and hydrogen storage systems. Each source is modelled in the quasi-dynamic domain, where a set of selected state variables is taken into account. The load is derived from the ship mission profile through a stochastic process that considers the ship speed. Different power management strategies are tested to exploit the various resources while maximizing the operating condition requirements. A set of key performance indicators, such as the CO2 emissions, the weight, and volume of the equipment, allows to compare the various power plant configurations so that the preliminary design solution can be identified

    Impacts of a weakened AMOC on precipitation over the Euro-Atlantic region in the EC-Earth3 climate model

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    Given paleoclimatic evidence that the Atlantic Meridional Overturning Circulation (AMOC) may affect the global climate system, we conduct model experiments with EC-Earth3, a state-of-the-art GCM, to specifically investigate, for the first time, mechanisms of precipitation change over the Euro-Atlantic sector induced by a weakened AMOC. We artificially weaken the strength of the AMOC in the model through the release of a freshwater anomaly into the Northern Hemisphere high latitude ocean, thereby obtaining a similar to 57% weaker AMOC with respect to its preindustrial strength for 60 model years. Similar to prior studies, we find that Northern Hemisphere precipitation decreases in response to a weakened AMOC. However, we also find that the frequency of wet days increases in some regions. By computing the atmospheric moisture budget, we find that intensified but drier storms cause less precipitation over land. Nevertheless, changes in the jet stream tend to enhance precipitation over northwestern Europe. We further investigate the association of precipitation anomalies with large-scale atmospheric circulations by computing weather regimes through clustering of geopotential height daily anomalies. We find an increase in the frequency of the positive phase of the North Atlantic Oscillation (NAO+), which is associated with an increase in the occurrence of wet days over northern Europe and drier conditions over southern Europe. Since a similar to 57% reduction in the AMOC strength is within the inter-model range of projected AMOC declines by the end of the twenty-first century, our results have implications for understanding the role of AMOC in future hydrological changes

    Silicone Oil Tamponade Removal: Which Technique Is More Effective? An X-Ray Photoemission Spectroscopy Study

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    Purpose: To compare the efficacy of two surgical techniques used to remove silicone oil (SiO) emulsion tamponade after pars plana vitrectomy: triple air‚Äďfluid exchange (AFX) and balanced salt solution lavage (BSSL). Methods: X-ray photoemission spectroscopy measured silicon content of the dry residue of fluid samples taken during AFX and BSSL. Ten patients underwent AFX and five BSSL. Three fluid samples were taken per patient, and the dry residue of 10 drops per sample were analyzed. A fluid sample from a patient who never received SiO tamponade was also analyzed to set a ‚Äúblank‚ÄĚ reference sample. Results: Patients‚Äô demographics showed no significant difference. Sample 1 of the two groups contained comparable silicon content while samples 2 and 3 of the AFX group contained significantly more silicon than that of the BSSL group (15.0 ¬Ī 0.1 and 12.0 ¬Ī 0.9 for the AFX group vs. 10.7 ¬Ī 1.4 and 5.2 ¬Ī 0.6 for the BSSL group, respectively; P < 0.05). The cumulative amount of silicon in the three successive samples was also significantly higher for the AFX group (42.3 ¬Ī 1.6 vs. 32 ¬Ī 2; P < 0.0001). The average silicon content ratio of consecutive samples was significantly higher for the AFX group compared to the BSSL group (0.90 ¬Ī 0.01 vs. 0.58 ¬Ī 0.06; P = 0.006). Conclusions: Triple AFX removed more silicon than triple lavage. The eye wall actively interacts with silicon emulsion retaining silicon content rather than behaving as a neutral container. Translational Relevance: Triple air‚Äďfluid exchange removed more silicon than BSS lavage. Neither technique behaved as a well-mixed box dilution, suggesting the eye walls actively retain emulsion and a dynamic equilibrium is established between silicon dispersion and the eye wall surface

    Antimicrobial and Antibiofilm Activities of Carvacrol, Amoxicillin and Salicylhydroxamic Acid Alone and in Combination vs. Helicobacter pylori: Towards a New Multi-Targeted Therapy

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    The World Health Organization has indicated Helicobacter pylori as a high-priority pathogen whose infections urgently require an update of the antibacterial treatments pipeline. Recently, bacterial ureases and carbonic anhydrases (CAs) were found to represent valuable pharmacological targets to inhibit bacterial growth. Hence, we explored the underexploited possibility of developing a multiple-targeted anti-H. pylori therapy by assessing the antimicrobial and antibiofilm activities of a CA inhibitor, carvacrol (CAR), amoxicillin (AMX) and a urease inhibitor (SHA), alone and in combination. Minimal Inhibitory (MIC) and Minimal Bactericidal (MBC) Concentrations of their different combinations were evaluated by checkerboard assay and three different methods were employed to assess their capability to eradicate H. pylori biofilm. Through Transmission Electron Microscopy (TEM) analysis, the mechanism of action of the three compounds alone and together was determined. Interestingly, most combinations were found to strongly inhibit H. pylori growth, resulting in an additive FIC index for both CAR-AMX and CAR-SHA associations, while an indifferent value was recorded for the AMX-SHA association. Greater antimicrobial and antibiofilm efficacy of the combinations CAR-AMX, SHA-AMX and CAR-SHA against H. pylori were found with respect to the same compounds used alone, thereby representing an innovative and promising strategy to counteract H. pylori infections

    Non-Redundant near-field to far-field transformation techniques

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    Near-field (NF) measurement techniques and the related NF to far-field (FF) transformations are gaining interest for their ability to allow an accurate evaluation of the radiation characteristics of those antennas whose electric sizes do not make it possible to perform direct FF measurements in a controlled and reflection-free environment, such as the anechoic chamber. This book provides a comprehensive treatment of classical NF-FF transformation techniques and describes the significant improvements achieved in their performance by correctly applying the non-redundant sampling representations of antenna radiated electromagnetic (EM) fields. Non-Redundant Near-Field to Far-Field Transformation Techniques is designed to meet the needs of students of antenna NF measurements, as well as of engineers and physicists working in the area. It has been written keeping in mind the fulfilment of two main objectives. The former is to provide all the analytical details on the derivation of the classical NF-FF transformation techniques without and with probe compensation, which are not easily available elsewhere in the literature. The latter is to give a comprehensive description of effective representations of the EM fields radiated over arbitrary rotational surfaces, which make use of a non-redundant (i.e., minimum) number of samples collected on these surfaces or along proper spirals wrapping them

    Green Smart Port Energy System Design: Optimal Sizing

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    The paper proposes an optimal sizing methodology for a green smart port with the presence of renewable energy resources and two energy carriers: electricity and hydrogen. The problem solution provides the optimal size of all the resources, taking into account a defined optimal energy management strategy. A notional smart port equipped with renewable resources has been used as study case, with the inclusion of data obtained from a real port to emulate the shore to ship power demand. The results show that the management strategy highly affect the optimal sizing solution, being the capital cost counterbalanced by the operational cost in an open market scenario where energy can be exported from the smart port to the national grid

    Association between Sex-Related ALOX5 Gene Polymorphisms and Lung Atopy Risk

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    Atopy is an exaggerated IgE-mediated immune response to foreign antigens in which metabolic abnormalities of the leukotrienes (LTs) pathway play a crucial role. Recent studies have described sex as a key variable in LT biosynthesis, partly explaining why treatment with anti-LT drugs in atopic subjects leads to better control of symptoms in women. In addition, variability in LT production is often associated with single nucleotide polymorphisms (SNPs) in the arachidonate 5-lipoxygenase (ALOX5) gene, which encodes the leukotriene-synthesizing enzyme machinery, 5-lipoxygenase (5-LO). This study aimed to investigate whether two SNPs of ALOX5 are implicated in sex differences in allergic diseases in a prospective cohort of 150 age- and sex-matched atopic and healthy subjects. Rs2029253 and rs2115819 were genotyped using allele-specific RT-PCR, and serum levels of 5-LO and LTB4 were measured by ELISA. Both polymorphisms are significantly more common in women than in men, and their influences on LT production vary as a function of sex, leading to a decrease in men’s and an increase in women’s serum levels of 5-LO and LTB4. These data represent a new resource for understanding sex-related differences in lung inflammatory diseases, partly explaining why women are more likely to develop allergic disorders than men
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