26 research outputs found

    Representative photomicrographs of full-thickness inflamed rat colon showing the distribution pattern of collagen fibers stained with van Gieson (VG, A-D), Sirius Red (SR, E-H) or Sirius Red/Fast Green (SR/FG, I-L).

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    <p>CM and LM: circular and longitudinal muscle, respectively; MP: myenteric plexus. Scale bars represent 50μm. Scatter plots show the percentage of positive pixel (PPP) of collagen ± SEM obtained from 6 rats in the whole colonic wall (M) and <i>tunica muscularis</i> (N). *,° P = 0.028 <i>versus</i> VG and SR, respectively.</p

    Representative photomicrographs of haematoxylin/eosin-stained full-thickness colonic samples from normal rats (A,B), or rats with DNBS-induced colitis at day 6 (C,D).

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    <p>Normal colon displays a normal morphological architecture of the wall and myenteric ganglia. In the inflamed colon, the following alterations are evident: infiltrated <i>tunica submucosa</i>; thickened and infiltrated <i>tunica muscularis</i> and <i>serosa</i>; vacuolated myenteric ganglia with altered cells and abundant eosinophilic infiltrations along the myenteric ridge (D, arrows and arrowheads, respectively). Scale bars represent 50 μm.</p

    Positive control tissues for immunohistochemistry.

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    <p>(A,B) The crypts of normal human colon were selected as control positive structures for Cx26 (A) and Cx43 (B): immunoreactivity is evident in the ephitelial cells of crypts (arrows). (C) Human myocardium was used as positive control for pS368-Cx43: immunoreactivity is localized at level of intercalated disks (arrows); immunoreactivity of myocardium for Cx43 is displayed in the box. (D) A human myenteric neuron, taken as a positive control cell for PKC, displays an intense PKCps immunoreaction. (E) A granulocyte of normal colon, chosen as positive control for RhoA, is markedly immunoreactive. (F) Mast cells (arrows), locked in the <i>tunica muscularis</i> of human normal colon, show a strong immunoreaction for c-Kit. In the same field an intramuscular c-Kit positive ICC is also evident (arrowhead). Scale bar: 50 µm.</p

    RhoA immunostaining and quantitative expression in the neuromuscular compartment of normal and diverticular disease human colon.

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    <p>In normal colon, RhoA is expressed in the circular (CM) and longitudinal muscle (LM) (A), while it is reduced in DD (B); scale bar: 100 µm. At higher magnification, SMCs show a dotted immunoreactivity localized at the membrane (arrows) and cytoplasmic (asterisks) level in normal colon (C,E), while in DD samples the localization is mainly evident in the cytoplasm (asterisks) (D,F); scale bars: 50 µm. (G) Image analysis of RhoA expression. Each column represents the PPP mean ± SD (9</p

    Smooth muscle α-actin (α-SMA) immunostaining.

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    <p>α-SMA is equally expressed in circular (CM) and longitudinal muscle (LM) of normal (A) and diverticular disease (DD) (B) human colon. Scale bar: 100 µm.</p

    PKC phosphorylated substrates immunostaining and quantitative analysis in the neuromuscular compartment of normal and diverticular disease human colon.

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    <p>In normal colon, PKCps immunostaining is expressed in both circular (CM) and longitudinal muscle (LM) (A), while it is reduced in DD (B); scale bars: 100 µm. Myenteric neurons (arrows) are intensely immunostained. (C) Image analysis of PKCps expression. Each column represents the PPP mean ± SD (9</p

    Immunostaining and quantitative analysis of ICCs in neuromuscular compartment of normal and diverticular disease human colon.

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    <p>Colonic specimens from DD patients contain fewer c-Kit positive and nuclear fast red-counterstained ICCs, with blunted and shortened branching (B, D, F), as compared to normal colon. Scale bars: 100 µm (A, B) and 50 µm (C–F). (G) Column graphs display ICC counting which is given as means ± SD of individual ICC densities normalized to area for ICC-CM (C) and ICC-LM (I).*P<.05,significant difference vs normal colon.</p

    Cx26 immunostaining and quantitative analysis in the neuromuscular compartment of normal and diverticular disease human colon.

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    <p>In normal colon, Cx26 is widely expressed in both circular (CM) and longitudinal muscle (LM) (A), while it is decreased in samples from DD colon (B); scale bar: 100 µm. At higher magnification (C–F), SMCs show a punctuated immunoreactivity, which is localized at the membrane (arrows), cytoplasmic (asterisks), paranuclear and nuclear (arrowheads) levels; scale bars: 50 µm. (G) Image analysis of Cx26 expression. Each column represents the PPP mean ± SD (9</p
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