On local existence and blow-up of solution for the higher-order nonlinear Kirchhoff-type equation with nonlinear strongly damped terms

In this paper ,we deal with the initial boundary value problems for higher -order kirchhoff-type equation with nonlinear strongly dissipation:At first ,we prove the local existence and uniqueness of the solution by Galerkin methodthen and contracting mapping principle .Furthermore,we prove the global existence of solution , At last,we consider that blow up of solution in finite time under suitable condition

Integrative network analysis of rifampinregulated miRNAs and their functions in human hepatocytes

Rifampin is an important drug used in the treatment of tuberculosis, and it increases the drug metabolism in human hepatocytes. Previous studies have shown that rifampin can indirectly influence drug deposition through the regulation of molecular interactions of miRNA, PXR and other genes. The potential functions of miRNAs associated with rifampin- induced drug disposition are poorly understood. In this study, significantly differentially expressed miRNAs (SDEM) were extracted and used to predict the miRNA-regulated co-expression target genes (MCeTG). Additionally, a miRNA-regulated co-expressed protein interaction network (MCePIN) was constructed for SDEM by extending from the protein interaction network (PIN). The functioning of the miRNAs were analyzed using GO analysis and KEGG pathway enrichment analysis. A total of 20 miRNAs belonging to SDEM were identified, and 632 miRNA-regulated genes were predicted. The MCePIN was constructed by extending from PIN, and 10 miRNAs and 33 genes that are relevant to 7 functions, including response to wounding, wound healing, response to drug, defense response, inflammatory response, liver development and drug metabolism, were discerned. The results provided by this study offer valuable insights into the effect of rifampin on miRNAs, genes and protein levels

Identification and Functional Analysis of ThADH1 and ThADH4 Genes Involved in Tolerance to Waterlogging Stress in Taxodium hybrid ‘Zhongshanshan 406’

The Taxodium hybrid ‘Zhongshanshan 406’ (T. hybrid ‘Zhongshanshan 406’) [Taxodium mucronatum Tenore × Taxodium distichum (L.). Rich] has an outstanding advantage in flooding tolerance and thus has been widely used in wetland afforestation in China. Alcohol dehydrogenase genes (ADHs) played key roles in ethanol metabolism to maintain energy supply for plants in low-oxygen conditions. Two ADH genes were isolated and characterized—ThADH1 and ThADH4 (GenBank ID: AWL83216 and AWL83217—basing on the transcriptome data of T. hybrid ‘Zhongshanshan 406’ grown under waterlogging stress. Then the functions of these two genes were investigated through transient expression and overexpression. The results showed that the ThADH1 and ThADH4 proteins both fall under ADH III subfamily. ThADH1 was localized in the cytoplasm and nucleus, whereas ThADH4 was only localized in the cytoplasm. The expression of the two genes was stimulated by waterlogging and the expression level in roots was significantly higher than those in stems and leaves. The respective overexpression of ThADH1 and ThADH4 in Populus caused the opposite phenotype, while waterlogging tolerance of the two transgenic Populus significantly improved. Collectively, these results indicated that genes ThADH1 and ThADH4 were involved in the tolerance and adaptation to anaerobic conditions in T. hybrid ‘Zhongshanshan 406’

Antiferromagnetic to Ferrimagnetic Phase Transition and Possible Phase Coexistence in Polar Magnets (Fe1−x_{1-x}Mnx_x)2_2Mo3_3O8_8

In the present work, magnetic properties of single crystal (Fe$_{1-x}$Mn$_x$)$_2$Mo$_3$O$_8$ ($0<x<1$) have been studied by performing extensive measurements. A detailed magnetic phase diagram is built up, in which antiferromagnetic state dominates for $x<0.25$ and ferrimagnetic phase arises for $x>0.3$. Meanwhile, sizeable electric polarization of spin origin is commonly observed in all samples, no matter what the magnetic state is. For the samples hosting a ferrimagnetic state, square-like magnetic hysteresis loops are revealed, while the remnant magnetization and coercive field can be tuned drastically by simply varying the Mn-content or temperature. Possible coexistence of the antiferromagnetic and ferrimagnetic phases is proposed to be responsible for the remarkable modulation of magnetic properties in the samples

Finite element analysis and experimental validation of the thermomechanical behavior in laser solid forming of Ti-6Al-4V

A three-dimensional (3D) thermomechanical&nbsp;coupled model&nbsp;for Laser Solid Forming (LSF) of Ti-6Al-4V alloy&nbsp;has been calibrated through experiments of 40-layers&nbsp;metal deposition&nbsp;using different scanning strategies. The sensitivity analysis of the mechanical parameters shows that the thermal expansion coefficient as well as the&nbsp;elastic limit&nbsp;of&nbsp;Ti-6Al-4V&nbsp;have a great impact on the mechanical behavior. Using the validated model and optimal mechanical parameters, the evolution of thermo-mechanical fields in LSF has been analyzed. It has been found that the stresses and distortions develop in two stages, after the deposition of the first layer and during the cooling phase after the manufacturing of the component. The cooling phase is the responsible of 70% of the&nbsp;residual stresses&nbsp;and 60% of the total distortions. The analyses indicate that by controlling the initial&nbsp;substrate temperature&nbsp;(pre-heating phase) and the final cooling phase it is possible to mitigate both distortion and residual stresses. Hence, the influence of different pre-heating procedures on the&nbsp;mechanical fields&nbsp;has been analyzed. The results show that increasing the pre-heating temperature of the substrate is the most effective way to reduce the distortions and residual stresses in&nbsp;Additive Manufacturing

Identification of rifampin-regulated functional modules and related microRNAs in human hepatocytes based on the protein interaction network

BACKGROUND: In combination with gene expression profiles, the protein interaction network (PIN) constructs a dynamic network that includes multiple functional modules. Previous studies have demonstrated that rifampin can influence drug metabolism by regulating drug-metabolizing enzymes, transporters, and microRNAs (miRNAs). Rifampin induces gene expression, at least in part, by activating the pregnane X receptor (PXR), which induces gene expression; however, the impact of rifampin on global gene regulation has not been examined under the molecular network frameworks. METHODS: In this study, we extracted rifampin-induced significant differentially expressed genes (SDG) based on the gene expression profile. By integrating the SDG and human protein interaction network (HPIN), we constructed the rifampin-regulated protein interaction network (RrPIN). Based on gene expression measurements, we extracted a subnetwork that showed enriched changes in molecular activity. Using the Kyoto Encyclopedia of Genes and Genomes (KEGG), we identified the crucial rifampin-regulated biological pathways and associated genes. In addition, genes targeted by miRNAs that were significantly differentially expressed in the miRNA expression profile were extracted based on the miRNA-gene prediction tools. The miRNA-regulated PIN was further constructed using associated genes and miRNAs. For each miRNA, we further evaluated the potential impact by the gene interaction network using pathway analysis. RESULTS AND DISCCUSSION: We extracted the functional modules, which included 84 genes and 89 interactions, from the RrPIN, and identified 19 key rifampin-response genes that are associated with seven function pathways that include drug response and metabolism, and cancer pathways; many of the pathways were supported by previous studies. In addition, we identified that a set of 6 genes (CAV1, CREBBP, SMAD3, TRAF2, KBKG, and THBS1) functioning as gene hubs in the subnetworks that are regulated by rifampin. It is also suggested that 12 differentially expressed miRNAs were associated with 6 biological pathways. CONCLUSIONS: Our results suggest that rifampin contributes to changes in the expression of genes by regulating key molecules in the protein interaction networks. This study offers valuable insights into rifampin-induced biological mechanisms at the level of miRNAs, genes and proteins