KŪNQǓ IN PRACTICE: A CASE STUDY

Ph.D

Automatic Objects Removal for Scene Completion

With the explosive growth of web-based cameras and mobile devices, billions of photographs are uploaded to the internet. We can trivially collect a huge number of photo streams for various goals, such as 3D scene reconstruction and other big data applications. However, this is not an easy task due to the fact the retrieved photos are neither aligned nor calibrated. Furthermore, with the occlusion of unexpected foreground objects like people, vehicles, it is even more challenging to find feature correspondences and reconstruct realistic scenes. In this paper, we propose a structure based image completion algorithm for object removal that produces visually plausible content with consistent structure and scene texture. We use an edge matching technique to infer the potential structure of the unknown region. Driven by the estimated structure, texture synthesis is performed automatically along the estimated curves. We evaluate the proposed method on different types of images: from highly structured indoor environment to the natural scenes. Our experimental results demonstrate satisfactory performance that can be potentially used for subsequent big data processing: 3D scene reconstruction and location recognition.Comment: 6 pages, IEEE International Conference on Computer Communications (INFOCOM 14), Workshop on Security and Privacy in Big Data, Toronto, Canada, 201

N′-(5-Bromo-2-hy­droxy­benzyl­idene)-4-nitro­benzohydrazide methanol monosolvate

In the title compound, C14H10BrN3O4·CH4O, the benzohydrazide mol­ecule is nearly planar [maximum deviation = 0.110 (2) Å]. The mean planes of the two benzene rings make a dihedral angle of 8.4 (3)°. In the benzohydrazide mol­ecule, there is an intra­molecular O—H⋯N hydrogen bond and the NH group is hydrogen bonded to the methanol solvent mol­ecule. In the crystal, inter­molecular O—H⋯O hydrogen bonds involving the methanol solvent mol­ecule link the benzohydrazide mol­ecules to form chains which propagate along the a axis

Theoretical study on Λc+→ΛK+Kˉ0\Lambda_c^+ \to \Lambda K^+\bar{K}^0 decay and Ξ∗(1690)\Xi^*(1690) resonance

We present a theoretical study of $\Xi^*(1690)$ resonance in the $\Lambda_c^+ \to \Lambda K^+ \bar{K}^0$ decay, where the weak interaction part proceeds through the Cabibbo-favored process $c \to s + u\bar{d}$. Next, the intermediate two mesons and one baryon state can be constructed with a pair of $q\bar{q}$ with the vacuum quantum numbers. Finally, the $\Xi^*(1690)$ is mainly produced from the final state interactions of $\bar{K}\Lambda$ in coupled channels, and it is shown in the $\bar{K}\Lambda$ invariant mass distribution. Besides, the scalar meson $a_0(980)$ and nucleon excited state $N^*(1535)$ are also taken into account in the decaying channels $K^+\bar{K}^0$ and $K^+\Lambda$, respectively. Within model parameters, the $K^+ \bar{K}^0$, $\bar{K}^0 \Lambda$ and $K^+ \Lambda$ invariant mass distributions are calculated, and it is found that our theoretical results can reproduce well the experimental measurements, especially for the clear peak around $1690$ MeV in the $\bar{K}\Lambda$ spectrum. The proposed weak decay process $\Lambda_c^+ \to \Lambda K^+ \bar{K}^0$ and the interaction mechanism can provide valuable information on the nature of the $\Xi^*(1690)$ resonance.Comment: 9 pages, 5 figure

Noise in Genotype Selection Model

We study the steady state properties of a genotype selection model in presence of correlated Gaussian white noise. The effect of the noise on the genotype selection model is discussed. It is found that correlated noise can break the balance of gene selection and induce the phase transition which can makes us select one type gene haploid from a gene group.Comment: 8 pages, 4 figure

Current Reversals in a inhomogeneous system with asymmetric unbiased fluctuations

We present a study of transport of a Brownian particle moving in periodic symmetric potential in the presence of asymmetric unbiased fluctuations. The particle is considered to move in a medium with periodic space dependent friction. By tuning the parameters of the system, the direction of current exhibit reversals, both as a function of temperature as well as the amplitude of rocking force. We found that the mutual interplay between the opposite driving factors is the necessary term for current reversals.Comment: 9 pages, 7 figure

Maintaining CD4/CD8 ratio and Th1-CTL subsets of chimeric antigen receptor (CAR)-T cells in serum-free culture conditions

Chimeric antigen receptor (CAR) T cells therapy is a promising strategy that significantly controlled the progress of cancer diseases. CAR-T cells could kill cancer cells through cellular immune response; therefore, CD8+ cytotoxic T cells are critical for CAR-T cell therapy. However, recent papers reported that CD4+ T helper cells were important for the response and maintenance of CAR-T cells in vivo. Here, we developed a serum-free CAR-T cell preparation process that maintained the T cell population and controlled the T cell subsets. The CD4+ and CD8+ T cell population in CAR-T cells were maintained at averagely 59.4 % and 34.6%, and the major T cell subsets were Th1 cells and cytotoxic T lymphocytes (CTLs), implying the potentially high cellular immune response. To verifying whether the prepared CAR-T cells were exhausted, the expression of several immune checkpoint markers was determined. Of interest, only less than 20% of CAR-T cells at endpoint were PD-1+ or CTLA4+, but more than 40% of CAR-T cells at the endpoint were TIM-3+, implying most CAR-T cells were not exhausted. These CAR-T cells produced more than 1 ng/mL of IFN-γ in the response to the antigen. Altogether, CAR-T cells could be prepared in our serum-free process in the controlling of T cell subsets, leading to potential high therapeutic potency. Please click Additional Files below to see the full abstract

THE PREDICTION OF FATIGUE-RELATED MUSCLE ADAPTATION IN DIFFERENT WORKLOAD DURING CYCLING ROWING EXERCISE

The purpose of this study was to identify the fatigue-related muscles during the different percentage of the maximal workload. Seven subjects were recruited and, firstly, performed the maximal workload test for 3 minutes. After 24 hours, the different workload tests (60%, 70% and 80% of the maximal workload) were used to reveal the fatigue-related muscle. The result showed that the biceps and triceps had the extreme contribution at the minor and greater workload. However, the neuromuscular fatigue threshold (NFT) is to predict the possibly fatigue muscle condition during the entire exercise. And the most contribution of the EMG expression didn’t stand for the greater value of the EMG number

In vitro high expansion of chimeric antigen receptor (CAR)-T cells in serum-free process conditions

Manufacturing process is an important and complex factor for preparing chimeric antigen receptor (CAR) T cells for therapy. Although serum was widely applied in the culture or expansion of T cells, the quality of serum could be varied from batch to batch, leading to the variation of T cell expansion and quality. In addition, the safety of pathogens from serum and Chemistry, Manufacturing, and Control (CMC) were required to be considered. To overcome the disadvantages of serum application in T cell culture, serum-free and xeno-free culture conditions were required. We intended to develop a rapid serum-free culture condition for the expansion of immune T cells ex vivo. In our optimized serum-free condition, CAR-T cells could be expanded to about 100-200 times to the initial cell number after 6-day culture and the cell viability of all specimens was above 98%. Of interest, the percentage of CAR+ population in all specimens was increases, and the T cell pollutions could be maintained at averagely about 35-40% of CD8+ T cells and averagely about 50-55% of CD4+ T cells after culture. Taken together, our conditions could be applied in the expansion of CAR-T cells for cell therapy to support the minimum requirement of blood or cell samples from patients and to maintain the T cell population. Please click Additional Files below to see the full abstract