Nodal network generator for CAVE3

A new extension of CAVE3 code was developed that automates the creation of a finite difference math model in digital form ready for input to the CAVE3 code. The new software, Nodal Network Generator, is broken into two segments. One segment generates the model geometry using a Tektronix Tablet Digitizer and the other generates the actual finite difference model and allows for graphic verification using Tektronix 4014 Graphic Scope. Use of the Nodal Network Generator is described

CAVE3: A general transient heat transfer computer code utilizing eigenvectors and eigenvalues

The method of solution is a hybrid analytical numerical technique which utilizes eigenvalues and eigenvectors. The method is inherently stable, permitting large time steps even with the best of conductors with the finest of mesh sizes which can provide a factor of five reduction in machine time compared to conventional explicit finite difference methods when structures with small time constants are analyzed over long time periods. This code will find utility in analyzing hypersonic missile and aircraft structures which fall naturally into this class. The code is a completely general one in that problems involving any geometry, boundary conditions and materials can be analyzed. This is made possible by requiring the user to establish the thermal network conductances between nodes. Dynamic storage allocation is used to minimize core storage requirements. This report is primarily a user's manual for CAVE3 code. Input and output formats are presented and explained. Sample problems are included which illustrate the usage of the code as well as establish the validity and accuracy of the method

Theory of Q-degradation and nonlinear effects in Nb-coated superconducting cavities

Amplitude-dependent absorption of RF power in superconducting cavity with a wall of normal metal (Cu ) covered by a film of superconductor (Nb ) is determined by three factors: (1)depairing effect of high-current density on superconducting energy gap; (2)increase of electromagnetic power penetrating to normal metal and Drude-absorbed in it, at higher RF amplitude; (3)heating of both superconducting coating and normal substrate resulting in increase of quasiparticle excitation density at higher RF power (P). Cavity Q-factor is calculated as a hnction of RF amplitude A = fi and is shown to follow a relationship 1n(Q/ Q,) = -const-P" with a=l at low temperature T T* where T* is characteristic temperature T* X TcS I d, S is the London penetration depth and d the thickness of superconducting coating

The Human SLC25A33 and SLC25A36 Genes of Solute Carrier Family 25 Encode Two Mitochondrial Pyrimidine Nucleotide Transporters

The human genome encodes 53 members of the solute carrier family 25 (SLC25), also called the mitochondrial carrier family, many of which have been shown to transport inorganic anions, amino acids, carboxylates, nucleotides, and coenzymes across the inner mitochondrial membrane, thereby connecting cytosolic and matrix functions. Here two members of this family, SLC25A33 and SLC25A36, have been thoroughly characterized biochemically. These proteins were overexpressed in bacteria and reconstituted in phospholipid vesicles. Their transport properties and kinetic parameters demonstrate that SLC25A33 transports uracil, thymine, and cytosine (deoxy)nucleoside di- and triphosphates by an antiport mechanism and SLC25A36 cytosine and uracil (deoxy)nucleoside mono-, di-, and triphosphates by uniport and antiport. Both carriers also transported guanine but not adenine (deoxy)nucleotides. Transport catalyzed by both carriers was saturable and inhibited by mercurial compounds and other inhibitors of mitochondrial carriers to various degrees. In confirmation of their identity (i) SLC25A33 and SLC25A36 were found to be targeted to mitochondria and (ii) the phenotypes of Saccharomyces cerevisiae cells lacking RIM2, the gene encoding the well characterized yeast mitochondrial pyrimidine nucleotide carrier, were overcome by expressing SLC25A33 or SLC25A36 in these cells. The main physiological role of SLC25A33 and SLC25A36 is to import/export pyrimidine nucleotides into and from mitochondria, i.e. to accomplish transport steps essential for mitochondrial DNA and RNA synthesis and breakdown

Transcriptional Regulation of the Mitochondrial Citrate and Carnitine/Acylcarnitine Transporters: Two Genes Involved in Fatty Acid Biosynthesis and E-oxidation

Transcriptional regulation of genes involved in fatty acid metabolism is considered the major long-term regulatory mechanism controlling lipid homeostasis. By means of this mechanism, transcription factors, nutrients, hormones and epigenetics control not only fatty acid metabolism, but also many metabolic pathways and cellular functions at the molecular level. The regulation of the expression of many genes at the level of their transcription has already been analyzed. This review focuses on the transcriptional control of two genes involved in fatty acid biosynthesis and oxidation: the citrate carrier (CIC) and the carnitine/ acylcarnitine/carrier (CAC), which are members of the mitochondrial carrier gene family, SLC25. The contribution of tissue-specific and less tissue-specific transcription factors in activating or repressing CIC and CAC gene expression is discussed. The interaction with drugs of some transcription factors, such as PPAR and FOXA1, and how this interaction can be an attractive therapeutic approach, has also been evaluated. Moreover, the mechanism by which the expression of the CIC and CAC genes is modulated by coordinated responses to hormonal and nutritional changes and to epigenetics is highlighte

Using object-based geomorphometry for hydro-geomorphological analysis in a Mediterranean research catchment

Abstract. The aim of the paper is to apply an object-based geomorphometric procedure to define the runoff contribution areas and support a hydro-geomorphological analysis of a 3 km2 Mediterranean research catchment (southern Italy). Daily and sub-hourly discharge and electrical conductivity data were collected and recorded during a 3-year monitoring activity. Hydro-chemograph analyses carried out on these data revealed a strong seasonal hydrological response in the catchment that differed from the stormflow events that occur in the wet periods and in dry periods. This analysis enabled us to define the hydro-chemograph signatures related to increasing flood magnitude, which progressively involves various runoff components (baseflow, subsurface flow and surficial flow) and an increasing contributing area to discharge. Field surveys and water table/discharge measurements carried out during a selected storm event enabled us to identify and map specific runoff source areas with homogeneous geomorphological units previously defined as hydro-geomorphotypes (spring points, diffuse seepage along the main channel, seepage along the riparian corridors, diffuse outflow from hillslope taluses and concentrate sapping from colluvial hollows). Following the procedures previously proposed and used by authors for object-based geomorphological mapping, a hydro-geomorphologically oriented segmentation and classification was performed with the eCognition (Trimble, Inc.) package. The best agreement with the expert-based geomorphological mapping was obtained with weighted plan curvature at different-sized windows. By combining the hydro-chemical analysis and object-based hydro-geomorphotype map, the variability of the contribution areas was graphically modeled for the selected event, which occurred during the wet season, by using the log values of flow accumulation that better fit the contribution areas. The results allow us to identify the runoff component on hydro-chemographs for each time step and calculate a specific discharge contribution from each hydro-geomorphotype. This kind of approach could be useful when applied to similar, rainfall-dominated, forested and no-karst catchments in the Mediterranean eco-region

Evidence for Non-Essential Salt Bridges in the M-Gates of Mitochondrial Carrier Proteins

Mitochondrial carriers, which transport metabolites, nucleotides, and cofactors across the mitochondrial inner membrane, have six transmembrane α-helices enclosing a translocation pore with a central substrate binding site whose access is controlled by a cytoplasmic and a matrix gate (M-gate). The salt bridges formed by the three PX[DE]XX[RK] motifs located on the odd-numbered transmembrane α-helices greatly contribute to closing the M-gate. We have measured the transport rates of cysteine mutants of the charged residue positions in the PX[DE]XX[RK] motifs of the bovine oxoglutarate carrier, the yeast GTP/GDP carrier, and the yeast NAD+ transporter, which all lack one of these charged residues. Most single substitutions, including those of the non-charged and unpaired charged residues, completely inactivated transport. Double mutations of charged pairs showed that all three carriers contain salt bridges non-essential for activity. Two double substitutions of these non-essential charge pairs exhibited higher transport rates than their corre-sponding single mutants, whereas swapping the charged residues in these positions did not increase activity. The results demonstrate that some of the residues in the charged residue positions of the PX[DE]XX[KR] motifs are important for reasons other than forming salt bridges, probably for playing specific roles related to the substrate interaction-mediated conformational changes leading to the M-gate opening/closing