The selection of the amount of anthracene derivatives from raw materials of plant origin and the study of their influence on the rat periodontal

The article presents a new method of ethanol extraction of anthracene derivatives from the herb St. John's wort, the total content of which was evaluated using a calibration graph. The drug improved mineral metabolism, showed anti-inflammatory effects in the bone tissue of the periodontal rats

The effect of anthracene derivatives on the state of the extracellular matrix of the periodontal connective tissue and the oral mucosa of old rats

In the experiments on 27 old female rats, the effect of the anthracene derivative preparation on the state of the intercellular matrix of the connective tissue of the periodontal and oral mucosa in intact animals, as well as on the periodontitis model, was studied

Оценка эффективности комплексного метода лечения скученности зубов

В статті представлені результати клініко-лабораторного обґрунтування комплексного лікування дітей зі скупченістю зубів у віці 12-14 років. З метою спрямованої регуляції процесів резорбції і остеогенезу кісткової тканини щелеп дітям поетапно призначали йодид калію, остеотропні препарати та адаптогени. Отримані результати стали підставою для рекомендацій щодо впровадження в стоматологічну практику розроблений лікувально-профілактичний комплекс, що дозволяє підвищити ефективність лікування скупченість положення зубів у дітей з постійним прикусом.Increase of effectiveness of orthodontic treatment of dentoalveolar anomalies at children in the period of a constant bite by a directional regulation of processes of a resorption and a bone formation of bone tissue of jaws with stage-by-stage use of Iodidum of a potassium, Osteotropic of a preparation and adaptogens .В статье представлены результаты клинико- лабораторного обоснования комплексного лечения исследования детей со скученностью зубов в возрасте 12-14 лет. С целью направленной регуляции процессов резорбции и остеогенеза костной ткани челюстей детям поэтапно назначали йодид калия, остеотропные препараты и адаптогены. Полученные результаты стали основанием для рекомендаций по внедрению в стоматологическую практику разработанный лечебно- профилактический комплекс, позволяющий повысить эффективность лечения скученного положения зубов у детей с постоянным прикусом

Psychosemantic analysis of eye care workers’ percepts of creative abilities

Background: This article puts the emphasis on the notion of “creative abilities” in the context of its interpretation by eye care workers. Creative potential is essential for the general and professional culture of physicians, and personal creativity takes on a new meaning in the age of digitalization and dominance of artificial intelligence in routine medical practice. Purpose: To investigate eye care workers’ percepts of creative abilities by conducting a psychosemantic analysis and considering these percepts as a source of health care workers’ job satisfaction. Material and Methods: The study sample was composed of the eye care workers from the Filatov Institute of Eye Diseases. Two hundred and eleven eye care workers were requested to take part in the study, and the response rate was 85.8% (181/211). The 181 responders included 99 nursing staff members and 82 physicians (ophthalmologists). The study was conducted with the use of Hughes and Powell’s Guiding Principles for Life (GPL) Questionnaire (adapted by Semeniuk), the Kim, Leong, and Lee’s Job Satisfaction Scale (JSS) (adapted by Semeniuk) and the Lüscher 8-color test. Results: Eye care workers rated “Creativity” low among the 22 “Guiding Principles for Life”. In physicians, such guiding principles as “Creativity”, “Devout”, and “Material wealth” were associated with each other, which indicates that the achievement of material wealth was associated with such a virtue as devout, and this achievement requires creativity. It was demonstrated that creativity as a guiding principle was more important for physicians than for the nursing staff, and in male physicians it was associated with unconscious satisfaction with working environment

PREDICTORS OF PROGRESSION OF CHRONIC PYELONEPHRITIS IN CHILDREN

Introduction The search for new diagnostic capabilities adverse forecasting of chronic pyelonephritis in children is a pressing issue today. To determine the degree of destruction of renal parenchyma, structural and functional changes in kidney informative study of fermenturiyi, analyze its relationship with albuminuria and profibrozuyuchymy growth factors. The aim was to identify predictors of progression of chronic pyelonephritis in children to assess the level of kidney function in dynamic observation. Materials and Methods: Pediatric Nephrology at the Department of SI «Institute of Nephrology AMS of Ukraine» at the CST №7 of Kyiv was examined 191 child over 5 years in age from 3 to 17 years. Results: In the group of children with progressive chronic pyelonephritis relapsing course 2fi-MG urine compared with groups of 2fi-MG there PNhighest level of reliable patients who had not frequent relapses and recovered (p <0.001). To determine infringement tubular renal function, analyzed the $2-MG urine and serum levels with enzyme activity NAG, NAG B and in patients with chronic pyelonephritis and established significant coefficients of paired correlation 2fi-MG serum and urine$2-MG between levels and activity Nag, NagB in urine. Discovered reliable positive correlation between levels of albuminuria and TGF-fi, which is profibrotychnym factor (r = 0,48, p <0.001) and identified a significant increase in its levels in patients with chronic pyelonephritis. Conducted stepwise multiple linear regression analysis revealed a reliable connection between the measure glomerular filtration rate decline per year (which is determined by progression) and $2-MG levels of blood TGF-^,$2-MG in urine, albuminuria and indicators renoscintigraphy - ENPstand% inclusion RFP180 min. Conclusions. Predictors of progression of pyelonephritis in children are: increased activity of lysosomal enzymes, maintaining elevated levels $2-MG serum and urine regardless of the degree of inflammatory activity in the kidneys and albuminuri

DYNAMIC CHANGES IN CARDIOVASCULAR RISK BIOMARKERS AND CYTOKINES OF MYOCARDITIS-FREE PATIENTS WITH DECOMPENSATED HEART FAILURE AND ISCHEMIC SYSTOLIC DYSFUNCTION

The development and progression of heart failure is associated with a variety of pathophysiological mechanisms, of particular interest is the study of the inflammatory response as a fundamental link in the pathogenesis of CHF and its main component – decompensation. An open, non-randomized, prospective study was carried out to evaluate the clinical and morphological features of subclinical inflammation in patients with acute decompensation of ischemic chronic heart failure with a reduced ejection fraction. The study included 25 patients with decompensated ischemic CHF with left ventricular ejection fraction &lt; 40% aged 35 to 75 years (60.12±9.3 y. o.). In this study the dynamics of the serum content of C-reactive protein (CRP), N-terminal fragment of the brain natriuretic peptide precursor protein (NT-proBNP), soluble ST2(sST2), insulin-like growth factor-1 receptor (IGF-1R), interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-α (TNFα) was performed by multiplex immunoassay using the FLEXMAP 3D. All studied patients were divided into two groups depending on the diagnosed myocarditis: patients with no signs of myocarditis and patients with myocarditis. It was found that in the group of patients with diagnosed myocarditis there was an increased content of CRP, IGF-1R, IL-6 and IL-10, TNFα compared to the group of patients without myocarditis. The median concentrations of the NT-proBNP and sST2 in both groups did not differ. At the follow-up visit a year later, there was a decrease in the content of CRP, NT-proBNP, IL-6 in both groups. In the group of patients with myocarditis, an increase in the content of sST2, IGF-1R, IL-10 was observed. Thus, the study carried out in dynamics revealed significant differences in the degree of changes in the serum activity of pro- and anti-inflammatory cytokines and biomarkers of cardiovascular risk in patients with decompensated heart failure with systolic dysfunction with diagnosed myocarditis and in its absence

Eotaxin and cardio-ankle vascular index in patients with high and very high cardiovascular risk

Eotaxin is a chemokine, which is a chemoattractant mainly to eosinophils, as well as basophils and Th2 lymphocytes. According to studies, overexpression of eotaxin is found in endothelial and smooth muscle cells of blood vessels in the area of atherosclerotic plaque. In clinical medicine, cardio-ankle vascular index (CAVI) is widely used as an indicator of arteriosclerosis and a predictor of cardiovascular events. Few studies have shown the relationship of eotaxin with coronary atherosclerosis; in other studies, the relationship of eotaxin with atherosclerosis, myocardial infarction and pulse wave velocity was not revealed. The aim of the present study was to assess blood level of eotaxin and cardio-ankle vascular index and their association with major cardiovascular risk factors in patients with high and very high cardiovascular risk. We examined 65 patients with high and very high cardiovascular risk, due to documented coronary artery disease, type 2 diabetes mellitus, or combination of cardiovascular risk factors and who were undergoing generally accepted cardioactive, hypoglycemic therapy and lipid-lowering therapy. All patients were examined for the elastic properties of the vascular wall by volumetric sphygmography with assessment of CAVI. In the blood, the concentrations of eotaxin, high-sensitivity C-reactive protein, glycosylated hemoglobin and lipid spectrum indicators were determined. All examined were divided into two groups: with a normal value of CAVI (less than 8) and elevated. Patients with elevated CAVI had higher concentrations of eotaxin (p = 0.013), total cholesterol (p = 0.009), low-density lipoprotein cholesterol (p = 0.016), were older (p &lt; 0.0001) and less likely to take statins (p = 0.002). In all those examined, correlations were found between serum eotaxin concentration and CAVI (rs = 0.34; p = 0.005), as well as age (rs = 0.32; p = 0.006). The age of the patients correlated with CAVI (rs = 0.35; p = 0.007). Thus, in our study, we for the first time showed the relationship between higher concentrations of eotaxin and an increased cardio-ankle vascular index in patients with high and very high cardiovascular risk. Cardio-ankle vascular index was associated with age, lipid metabolism and lipid-lowering therapy. The obtained results allow us to consider eotaxin as a factor associated with atherogenesis and arterial stiffness

IMMUNOREGULATORY IMBALANCE AND FUNCTIONAL STATE OF THE HEART IN THE PATIENTS WITH DIABETES MELLITUS TYPE 2

Diabetes mellitus type 2 is one of the most important non-infectious diseases in the modern world, being an important risk factor of cardiovascular disorders. Changes in left ventricular myocardial diastolic function are observed in diabetic patients independently from other comorbidities. Etiology of the heart failure during diabetes mellitus type 2 is multifactorial, exhibiting cellular,  molecular and metabolic aspects. However, its pathophysiological mechanisms are not completely understood. The aim of this study was to evaluate numbers of inflammatory T lymphocytes, i.e., T helper type 1 (Th1) and T helper type 17 (Th17) cells, and FoxP3+T regulatory lymphocytes, depending on the functional state of the heart assessed by two-dimensional echocardiography in patients with arterial hypertension and diabetes mellitus type 2. A total of twenty-five patients with a combination of arterial hypertension and diabetes mellitus type 2, and 14 patients with arterial hypertension without carbohydrate disturbances were recruited to a cross-ectional case-control study. All the patients underwent echocardiography with transthoracic access at the M-mode, B-mode and Doppler mode of imaging. We evaluated numbers of Th1 and Th17 lymphocytes by intracellular production of IL-17 and IFNγ by CD4+ lymphocytes, respectively. The numbers of FoxP3+T regulatory lymphocytes were estimated by expression of CD25 and FoxP3 transcription factor. A flow cytometry approach was used in both cases. We revealed some correlations between the numbers of Th17 lymphocytes, FoxP3+T regulatory lymphocytes and functional parameters of myocardium in patients with diabetes mellitus type 2, which were absent in patientswithout carbohydrate impairments. The numbers of FoxP3+T egulatory lymphocytes, Treg/Th17 lymphocyte ratio, and mean fluorescence intensity of IL-17 for Th17 cells was lower in patients with diabetes mellitus and diastolic dysfunction compared to the patients with diabetes free of diastolic dysfunction. Association of diastolic dysfunction with diabetes mellitus type 2 was accompanied by increase of IFNγ+Th1 lymphocyte numbers and concentrations of IL-10, IFNγ and TNFα in serum as compared to the patients with diastolic dysfunction in the absence of carbohydrate metabolism disturbances. The diabetic patients with diastolic dysfunction were characterized by hyperinsulinemia, hyperglycemia, higher index of insulin resistance, increase of waist circumference and visceral adiposity index when compared to the patients with diastolic dysfunction without diabetes. Visceral obesity and decrease of insulin sensitivity may be regarded as pathogenetically significant factors for the development of immune regulatory imbalance and diastolic dysfunction in the patients with diabetes mellitus type 2

SUBCELLULAR LOCALIZATION OF FoxP3 TRANSCRIPTION FACTOR IN PATIENTS WITH ACUTE CORONARY SYNDROME: COMPARATIVE ANALYSIS AND PROSPECTIVE OBSERVATION

The key cellular and molecular factors being involved in the resolution of inflammation following acute myocardial infarction remain poorly understood. T-regulatory (Treg) lymphocytes are characterized by the extreme potential to regulate the strength and direction of immune responses during the myocardial injury. The functional activity of Treg-lymphocytes depends upon the transcription factor forkhead box protein P3 (FoxP3). It may be also expressed in conventional T-lymphocytes at the stage of their activation. Nuclear localization of FoxP3 is a prerequisite factor determining its ability to impact the suppressive functions of Treglymphocytes.The aim of the present study was comparative evaluation of FoxP3+T-lymphocytes frequency and counts, combined with estimation of FoxP3 subcellular localization, in patients with acute myocardial infarction and chronic coronary syndrome and examination of changes of these parameters in the short-term follow-up of patients with myocardial infarction. The study included 14 patients with chronic coronary syndrome (8 males; 6 females; 63.2±9.0 y.o.) and 5 patients with acute anterior ST-segment elevation myocardial infarction (4 males; 1 female; 61.4±11.2 y.o.) at days 1, 3 and 7 after the event. The frequency of FoxP3+ conventional and regulatory T-lymphocytes was evaluated in peripheral blood mononuclear cells together with estimation of the level of FoxP3 nuclear localization by imaging flow cytometry.Patients with infarction were characterized by the decreased counts of FoxP3+Treg-lymphocytes compared to patients with chronic coronary syndrome, and exhibited even further decrease in the counts of FoxP3+Tregcells at day 7 after infarction, while frequency of Treg and conventional T-lymphocytes did not differ significantly. The level of FoxP3 nuclear translocation was lower both in Treg and conventional T-lymphocytes in patients at day 1 post-infarction compared to patients with chronic coronary syndrome. Absolute counts of FoxP3+Tregs with nuclear FoxP3 localization remained significantly lower both at days 1 and 7 post-infarction compared to patients with chronic coronary syndrome.Thus, here we demonstrated that FoxP3 nuclear localization experiences decrease in the course of acute myocardial infarction and may serve as a more sensitive marker of changes in Treg-lymphocyte functioning than simple evaluation of their frequency

Оптимизация внутризаводских перевозок по контактному графику

For the English full text of the article please see the attached PDF-File (English version follows Russian version).ABSTRACT The presented article is a continuation of research on development of an alternative model for organization of transportation according to the contact schedule i n the conditions of a metallurgical enterprise. The mathematical model is based on a dynamic transport problem with delays (DTPD). It is shown in what way an optimal transportation plan is obtained and minimization of transportation costs is ensured with the agreed production programs of supply shops and consumer shops. The improvement of the system of transportation of empty cargo flow according to the contact schedule with the help of DTPD, as the authors argue, allows to abandon the strict assignment of cars to a specific transportation, supply of empty cars is carried out according to the most favorable option for a consumer (with a minimum of transport and production costs). The cars can be delivered to the consumer for loading onto the cargo front from under any used load. The use of a dynamic transportation problem with delays as the basis of a mathematical model for development of an optimal plan for transportation of cars according to the contact schedule helps to more than halve the transport and production costs of the shops of the metallurgical plant. Keywords: dynamic transport problem with delays, internal transportation, optimization of transportation, contact schedule, metallurgical plant, railway transport.Полный текст на англ. языке находится в прилагаемом файле ПДФ (англ. версия следует после русской версии).Представленная статья является продолжением исследований по разработке альтернативной модели организации перевозок по контактному графику в условиях металлургического предприятия. В основу математической модели положена динамическая транспортная задача с задержками (ДТЗЗ). Показано, каким путём получается оптимальный план перевозок и обеспечивается минимизация транспортных расходов при согласованных производственных программах цехов-поставщиков и цехов- потребителей