TableA1.xls

This appendix explains our sample sizes (i.e. number of tanks of fish), family sizes, and replicates for which there is missing data for either the pecking assay or opsin assay

tablea2.xls

This data set provides behavioral data for a pecking assay designed to assess the preferences of animals among different colors in a non-mating scenario. This data set provides the total number of pecks at red, orange, yellow, green, blue, white, and black dots summed across two replicate measures of pecking behavior for each tank in each testing environment. Some tanks only pecked on one occasion. For those, we simply report the number of pecks on that trial. Sire ID, Dam ID, Tank ID, Sire Population, Dam Population, and Rearing Environment are as reported in Table A1. Tanks with fewer than 5 total pecks in a given testing environment were excluded from the analysis

Data from: Teasing apart the many effects of lighting environment on opsin expression and foraging preference in bluefin killifish

Coloration and color vision covary with lighting in many taxa. Determining the mechanisms underlying these patterns is difficult because lighting environments can have multiple effects on signaling that occur at multiple time scales. Lighting environments can (1) immediately affect signal propagation and transmission which determine the radiance spectrum reaching the receiver, (2) induce variation in visual systems via developmental plasticity, and (3) lead to genetic differences in visual systems due to a history of selection in different habitats. We tease apart these effects on pecking preference and examine the relationship between pecking preference and opsin expression. Using killifish from two visually distinct populations (clear vs. tea-stained water), we performed crosses (genetics), raised animals under different lighting conditions (developmental plasticity), and assayed preference to peck at different colored dots under different lighting conditions (immediate effects). Pecks are interpreted as foraging preference. Developmental plasticity affected both pecking preference and opsin expression. Lighting environments also had immediate effects on pecking preference, but these depended on the lighting conditions animals experienced during development. Genetic effects were detected in opsin expression, but there were no corresponding effects on pecking preference. Overall, only 3.36% of the variation in pecking preference was accounted for by opsin expression

tablea2.xls

This data set provides behavioral data for a pecking assay designed to assess the preferences of animals among different colors in a non-mating scenario. This data set provides the total number of pecks at red, orange, yellow, green, blue, white, and black dots summed across two replicate measures of pecking behavior for each tank in each testing environment. Some tanks only pecked on one occasion. For those, we simply report the number of pecks on that trial. Sire ID, Dam ID, Tank ID, Sire Population, Dam Population, and Rearing Environment are as reported in Table A1. Tanks with fewer than 5 total pecks in a given testing environment were excluded from the analysis

tablea2.xls

This data set provides the total number of pecks at red, orange, yellow, green, blue, white, and black dots summed across two replicate measures of pecking behavior for each tank in each testing environment. Some tanks only pecked on one occasion. For those, we simply report the number of pecks on that trial. Sire ID, Dam ID, Tank ID, Sire Population, Dam Population, and Rearing Environment are as reported in Table A1. Tanks with fewer than 5 total pecks in a given testing environment were excluded from the analysis

Data from: Intensifying drought eliminates the expected benefits of elevated carbon dioxide for soybean

Stimulation of C3 crop yield by rising concentrations of atmospheric carbon dioxide ([CO2]) is widely expected to counteract crop losses that are due to greater drought this century. But these expectations come from sparse field trials that have been biased towards mesic growth conditions. This eight-year study used precipitation manipulation and year-to-year variation in weather conditions at a unique open-air field facility to show that the stimulation of soybean yield by elevated [CO2] diminished to zero as drought intensified. Contrary to the prevalent expectation in the literature, rising [CO2] did not counteract the effect of strong drought on photosynthesis and yield because elevated [CO2] interacted with drought to modify stomatal function and canopy energy balance. This new insight from field experimentation under hot and dry conditions, which will become increasingly prevalent in the coming decades, highlights the likelihood of negative impacts from interacting global change factors on a key global commodity crop in its primary region of production

Final_Data_Deposit

This directory contains the raw data and the code necessary to replicate the analyses in Gray et al (2016)