Distribution of cattle breed per period.

<p>Cattle sampled were of a range of different breeds found in the UK. 175 cattle were Aberdeen Angus cross, 118 were Limousin cross, 73 were Charolais cross, 48 were Aberdeen Angus, 37 were Simmental, 33 Holstein Friesian, 26 Limousin, 18 British Blue cross, 12 Charolais and 85 were of other less common breeds.</p

Predictive values of a) FEC and b) MHS over a range of prevalences.

<p>Prevalence estimates for each sampling period are shown by dotted lines.</p

Distribution of cattle age per period.

<p>The age of cattle sampled ranged from 369 to 1121, with a mean of 720 days old.</p

Mean posterior estimates and 95% BCIs.

<p>Estimates of the prevalence (pi), sensitivity (Se) and specificity (Sp) for each period (summer 2013 (A), winter 2014 (B), autumn 2014 (C)).</p

Proportions of test positives for each test and number of animals sampled.

<p>Proportions of test positives for each test and number of animals sampled.</p

Geographical distribution of cattle sampled.

<p>Samples used in this study were taken from Scotbeef, one of Scotland’s largest red meat abattoirs, receiving animals from all around Scotland, northern England and Northern Ireland. Figure shows the distribution of cattle sampled i.e the geographical distribution of the general population of cattle slaughtered at the abattoir was well represented. The map was plotted using R package <i>ggmap</i> [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0161621#pone.0161621.ref035" target="_blank">35</a>] using tiles sourced from Stamen Design (using data by OpenStreetMap).</p

Distribution of parasite counts by fibrosis score and MHS classification.

<p>Figure shows the distribution of parasite burden per fibrosis score as recorded during liver necropsy. Among livers where flukes were found, parasite burden ranged from 1 to 86 parasites, with a mean of 8.5 and a median of 4. A fibrosis score was assigned based on a presentation of the liver mimicking the one presented to the MHS. The colour of the points shows the decision taken by the MHS during liver inspection at the abattoir.</p

Mean estimates and 95% BCIs of the prevalence and test sensitivity and specificity per period.

<p>Mean estimates and 95% BCIs of the prevalence and test sensitivity and specificity per period.</p

Proportion of attendance to SP by distance to nearest SP.

<p>Figure shows that the proportion of attendance to SP decreases as distance to nearest SP increases.</p

Univariable analysis results of the association of each factor with attendance to SP.

<p>Univariable analysis results of the association of each factor with attendance to SP.</p