54 research outputs found
Novel, acid-labile, hydroxydiether lipid cores in methanogenic bacteria.
Polar ether lipids extracted from 15 methanogenic bacteria, representative of seven genera, were screened by nuclear magnetic resonance and thin layer chromatography for the presence of hydroxyl groups on the C20-phytanyl moieties. Major amounts of hydroxydiether core lipid were confirmed for Methanosaeta concilii and discovered in two Methanosarcina species, Methanococcus voltae, and tentatively in several Methanobacterium species. Signals at 1.24 and 1.8-1.9 ppm in 1H NMR spectra are characteristic of Methanosaeta concilii lipids hydroxylated on carbon-3 (sn-3 chain). Related signals, which were shifted slightly, appeared in spectra of the polar lipids extracted from both Methanosarcina species. Following mild hydrolysis to remove the polar head groups, only two chromatographically distinct core lipids were found in significant amounts in Methanosarcina barkeri (and Methanosarcina mazei) consisting of 43% 2,3-di-O-phytanyl-sn-glycerol (C20,20-diether) and 57% C20,20-hydroxydiether. This latter core lipid differed from the hydroxydiether from M. concilii by hydroxylation, on carbon-3, of the phytanyl chain in ether linkage to the sn-2 carbon of glycerol. The structural assignment was based on identification of the novel hydroxydiether core and its methylation products by 1H NMR, 13C NMR, and mass spectroscopy. The hydroxy core lipid degraded to various products during standard methanolic HCl and sulfuric acid procedures, including a methoxy derivative (methanolic HCl) and the 3-mono-O-phytanyl-sn-glycerol
Archaeosome lipid vesicles as new vaccine delivery systems
NRC publication: Ye
New ether lipid structures in methanogenic Archaeobacteria and their possible applications
NRC publication: Ye
Ammonia toxicity in pure cultures of methanogenic bacteria
NRC publication: Ye
Archaeosomes as adjuvant carriers for soluble proteins, tested in BALB/c mice
Peer reviewed: YesNRC publication: Ye
Cation toxicity and ammonium/potassium exchange in methanogenic bacteria
Peer reviewed: YesNRC publication: Ye
Stability of liposomes prepared from total polar lipids of Archaeobacteria and egg phospatidylcholine mixtures
Peer reviewed: YesNRC publication: Ye
Ionic requirements for methane production by washed cells of Methanothrix concilii GP6
Peer reviewed: YesNRC publication: Ye
In vitro evaluation of archaeosomes for oral delivery and vaccines
Peer reviewed: YesNRC publication: Ye
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