Induced Systemic Resistance (ISR) in Arabidopsis thaliana by Bacillus amyloliquefaciens and Trichoderma harzianum Used as Seed Treatments

International audienceThe Trichoderma fungal species and the bacteria Bacillus species were described as inducers of plant systemic resistance in relation to their antagonistic activity. The objective of this study was to evaluate the effect of selected strains of Bacillus amyloliquefaciens (I3) and Trichoderma harzianum (A) on inducing systemic resistance in Arabidopsis thaliana as a model for plant molecular genetics. The microorganisms were identified and were confirmed for their antagonistic potential in vitro and in vivo in previous studies. In order to explore this mechanism, two mutants of A. thaliana carrying a PR1 promoter (a conventional marker of salicylic acid (SA) pathway) and LOX2 promoter (a marker triggering jasmonic acid (JA) pathway activation) were analyzed after inoculating antagonists. Transgenic reporter line analysis demonstrated that B. amyloliquefaciens I3 and T. harzianum A induce A. thaliana defense pathways by activating SA and JA at a high level compared to lines treated with chemical elicitors of references (acibenzolar-S-methyl (Bion 50 WG (water-dispersible granule)), SA, and methyl jasmonate). The efficacy of B. amyloliquefaciens I3 and T. harzianum A in inducing the defense mechanism in A. thaliana was demonstrated in this study

Hybrid magneto-optical mode converter made with a magnetic nanoparticles-doped SiO2/ZrO2 layer coated on an ion-exchanged glass waveguide

International audienceThis paper describes the possibility to achieve a TE-TM mode conversion in a magneto-optical hybrid waveguide operating at k¼1550 nm. This hybrid device is made by coating a SiO2/ZrO2 layer doped with magnetic nanoparticles on an ion-exchanged glass waveguide. Soft annealing (90 C) and UV treatment, both compatible with the ion exchange process, have been implemented to finalize the magneto-optical film. Optical characterizations that have been carried out demonstrated the efficiency of these hybrid structures in terms of lateral confinement and mode conversion. Indeed, TE to TM mode conversion has been observed when a longitudinal magnetic field is applied to the device. The amount of this conversion is discussed taking into account the distribution of light between the layer and the guide, and the modal birefringence of the structure

IIAC-LAIOS – Laboratoire d’anthropologie des institutions et organisations sociales

Catherine Neveu, directrice de recherche au CNRS Anthropologie des processus de citoyenneté Cette année, le séminaire a principalement exploré la question générale des qualifications de la citoyenneté, à partir de deux de ces qualifications : les citoyennetés dites « ordinaire » et « urbaine ». Liée à la question plus large de « l’ordinaire » en politique, la notion de « citoyenneté ordinaire » a fait l’objet d’un questionnement tant conceptuel que méthodologique : comment cerner ce qui ferai..

IIAC-LAIOS – Laboratoire d’anthropologie des institutions et organisations sociales

Catherine Neveu, directrice de recherche au CNRS Anthropologie des processus de citoyenneté Cette année, le séminaire a principalement exploré la question générale des qualifications de la citoyenneté, à partir de deux de ces qualifications : les citoyennetés dites « ordinaire » et « urbaine ». Liée à la question plus large de « l’ordinaire » en politique, la notion de « citoyenneté ordinaire » a fait l’objet d’un questionnement tant conceptuel que méthodologique : comment cerner ce qui ferai..

Cross-Reactivity of Herpesvirus-Specific CD8 T Cell Lines Toward Allogeneic Class I MHC Molecules

Although association between persistent viral infection and allograft rejection is well characterized, few examples of T-cell cross-reactivity between self-MHC/viral and allogeneic HLA molecules have been documented so far. We appraised in this study the alloreactivity of CD8 T cell lines specific for immunodominant epitopes from human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV). CD8 T cell lines were generated after sorting with immunomagnetic beads coated with either pp65495–503/A*0201, BMLF1259–267/A*0201, or BZLF154–64/B*3501 multimeric complexes. Alloreactivity of the CD8 T cell lines against allogeneic class I MHC alleles was assessed by screening of (i) TNF-α production against COS-7 cells transfected with as many as 39 individual HLA class I-encoding cDNA, and (ii) cytotoxicity activity toward a large panel of HLA-typed EBV-transformed B lymphoblastoid cell lines. We identified several cross-reactive pp65/A*0201-specific T cell lines toward allogeneic HLA-A*3001, A*3101, or A*3201. Moreover, we described here cross-recognition of HLA-Cw*0602 by BZLF1/B*3501-specific T cells. It is noteworthy that these alloreactive CD8 T cell lines showed efficient recognition of endothelial cells expressing the relevant HLA class I allele, with high level TNF-α production and cytotoxicity activity. Taken together, our data support the notion that herpes virus-specific T cells recognizing allo-HLA alleles may promote solid organ rejection

Serge Tousignant : jeux d'espaces, jeux de regards : le catalogue

L'exposition qui fait l'objet de ce catalogue a malheureusement été annulée à cause de la pandémie, mais elle aurait dû se tenir au Carrefour des arts et des sciences de l’Université de Montréal du 8 avril au 12 juin 2020.Catalogue d'exposition préparé à l'hiver 2020 dans le cadre du cours HAR 6080 / MSL 6509 : Muséologie et histoire de l'art, donné par la professeure Christine Bernier.Catalogue préparé sous la direction de Christine Bernie

Mineral trioxyde aggregate versus calcium hydroxide in apexification of non vital immature teeth: Study protocol for a randomized controlled trial

<p>Abstract</p> <p>Background</p> <p>Pulp necrosis is one of the main complications of dental trauma. When it happens on an immature tooth, pulp necrosis implies a lack of root maturation and apical closure. A therapy called apexification is required to induce the formation of a calcified apical barrier allowing a permanent and hermetic root filling. The aim of this prospective randomized clinical trial is to compare Mineral Trioxide Aggregate(MTA)with Calcium Hydroxide(CH)as materials used to induce root-end closure in necrotic permanent immature incisors.</p> <p>Methods/Design</p> <p>This study, promoted by AP-HP, was approved by the ethics committee(CPP Paris Ile de France IV). 34 children aged from 6 to 18 years and presenting a non-vital permanent incisor are selected. Prior to treatment, an appropriate written consent has to be obtained from both parents and from children. Patients are then randomly assigned to either the MTA(experimental)or CH(control)groups. Recalls are performed after 3, 6 and 12 months to determine the presence or absence of a calcified apical barrier through the use of clinical and radiographic exams. Additional criteria such as clinical symptoms, apical radiolucencies, periapical index(PAI)are also noted.</p> <p>Trial registration</p> <p>ClinicalTrials.gov no. <a href="http://www.clinicaltrials.gov/ct2/show/NCT00472173">NCT00472173</a> (First inclusion: May 10, 2007; Last inclusion: April 23, 2009; study completed: April 15, 2010)</p

CC9 Livestock-Associated Staphylococcus aureus Emerges in Bloodstream Infections in French Patients Unconnected With Animal Farming

We report 4 bloodstream infections associated with CC9 agr type II Staphylococcus aureus in individuals without animal exposure. We demonstrate, by microarray analysis, the presence of egc cluster, fnbA, cap operon, lukS, set2, set12, splE, splD, sak, epiD, and can, genomic features associated with a high virulence potential in human