A calibration point for stellar evolution from massive star asteroseismology

Massive stars are progenitors of supernovae, neutron stars and black holes. During the hydrogen-core burning phase their convective cores are the prime drivers of their evolution, but inferences of core masses are subject to unconstrained boundary mixing processes. Moreover, uncalibrated transport mechanisms can lead to strong envelope mixing and differential radial rotation. Ascertaining the efficiency of the transport mechanisms is challenging because of a lack of observational constraints. Here we deduce the convective core mass and robustly demonstrate non-rigid radial rotation in a supernova progenitor, the $12.0^{+1.5}_{-1.5}$ solar-mass hydrogen-burning star HD 192575, using asteroseismology, TESS photometry, high-resolution spectroscopy, and Gaia astrometry. We infer a convective core mass ($M_{\rm cc} = 2.9^{+0.5}_{-0.8}$ solar masses), and find the core to be rotating between 1.4 and 6.3 times faster than the stellar envelope depending on the location of the rotational shear layer. Our results deliver a robust inferred core mass of a massive star using asteroseismology from space-based photometry. HD 192575 is a unique anchor point for studying interior rotation and mixing processes, and thus also angular momentum transport mechanisms inside massive stars.Comment: 41 pages, 5 figures, 1 table. Version comment: updated erroneous affiliatio

Quantitative Comparison of Constitutive Promoters in Human ES cells

BACKGROUND: Constitutive promoters that ensure sustained and high level gene expression are basic research tools that have a wide range of applications, including studies of human embryology and drug discovery in human embryonic stem cells (hESCs). Numerous cellular/viral promoters that ensure sustained gene expression in various cell types have been identified but systematic comparison of their activities in hESCs is still lacking. METHODOLOGY/PRINCIPAL FINDINGS: We have quantitatively compared promoter activities of five commonly used constitutive promoters, including the human β-actin promoter (ACTB), cytomegalovirus (CMV), elongation factor-1α, (EF1α), phosphoglycerate kinase (PGK) and ubiquitinC (UbC) in hESCs. Lentiviral gene transfer was used to ensure stable integration of promoter-eGFP constructs into the hESCs genome. Promoter activities were quantitatively compared in long term culture of undifferentiated hESCs and in their differentiated progenies. CONCLUSION/SIGNIFICANCE: The ACTB, EF1α and PGK promoters showed stable activities during long term culture of undifferentiated hESCs. The ACTB promoter was superior by maintaining expression in 75-80% of the cells after 50 days in culture. During embryoid body (EB) differentiation, promoter activities of all five promoters decreased. Although the EF1α promoter was downregulated in approximately 50% of the cells, it was the most stable promoter during differentiation. Gene expression analysis of differentiated eGFP+ and eGFP- cells indicate that promoter activities might be restricted to specific cell lineages, suggesting the need to carefully select optimal promoters for constitutive gene expression in differentiated hESCs

SLO-2 Is Cytoprotective and Contributes to Mitochondrial Potassium Transport

Mitochondrial potassium channels are important mediators of cell protection against stress. The mitochondrial large-conductance “big” K+ channel (mBK) mediates the evolutionarily-conserved process of anesthetic preconditioning (APC), wherein exposure to volatile anesthetics initiates protection against ischemic injury. Despite the role of the mBK in cardioprotection, the molecular identity of the channel remains unknown. We investigated the attributes of the mBK using C. elegans and mouse genetic models coupled with measurements of mitochondrial K+ transport and APC. The canonical Ca2+-activated BK (or “maxi-K”) channel SLO1 was dispensable for both mitochondrial K+ transport and APC in both organisms. Instead, we found that the related but physiologically-distinct K+ channel SLO2 was required, and that SLO2-dependent mitochondrial K+ transport was triggered directly by volatile anesthetics. In addition, a SLO2 channel activator mimicked the protective effects of volatile anesthetics. These findings suggest that SLO2 contributes to protection from hypoxic injury by increasing the permeability of the mitochondrial inner membrane to K+

Improved Air Quality Forecasting Report ISB52-08. Visualisation of Dual Doppler Lidar data

This report ISB52-08 was produced under Project 52 of the Invest to Save Scheme, or ISB. The objective of this project is to improve the ability of air quality forecasts and thus impact on the quality of life in and around urban areas

Improved air quality forecasting report ISB52-03. Identification of key flow parameters for visualisation

This report ISB52-03 was produced under Project 52 of the Invest to Save Budget, or ISB. The aim of this project is to improve atmospheric pollution dispersion models with the goal of improving air quality forecasting. During the project life, the team will be developing a better understanding of airflow near the earth’s surface, focussing especially on urban meteorology. This will be achieved through the gathering of accurate 3-Dimensional wind flow data using laser radars, also called lidars, and by incorporating that new knowledge into the dispersion models

Improved Air Quality Forecasting Report ISB52-06. Boundary Layer Measurements of Dispersion Model Parameters using Dual Doppler Lidar at Malvern, UK

This report ISB52-06 was produced under Project 52 of the Invest to Save Budget, or ISB. The aim of this project is to improve atmospheric pollution dispersion models with the goal of improving air quality forecasting. During the project life, the team will be developing a better understanding of airflow near the earth’s surface, focussing especially on urban meteorology. This will be achieved through the gathering of accurate 3-Dimensional wind flow data using laser radars, also called lidars, and by incorporating that new knowledge into the dispersion models

Visualization in meteorology --- A survey of techniques and tools for data analysis tasks

This article surveys the history and current state of the art of visualization in meteorology, focusing on visualization techniques and tools used for meteorological data analysis. We examine characteristics of meteorological data and analysis tasks, describe the development of computer graphics methods for visualization in meteorology from the 1960s to today, and visit the state of the art of visualization techniques and tools in operational weather forecasting and atmospheric research. We approach the topic from both the visualization and the meteorological side, showing visualization techniques commonly used in meteorological practice, and surveying recent studies in visualization research aimed at meteorological applications. Our overview covers visualization techniques from the fields of display design, 3D visualization, flow dynamics, feature-based visualization, comparative visualization and data fusion, uncertainty and ensemble visualization, interactive visual analysis, efficient rendering, and scalability and reproducibility. We discuss demands and challenges for visualization research targeting meteorological data analysis, highlighting aspects in demonstration of benefit, interactive visual analysis, seamless visualization, ensemble visualization, 3D visualization, and technical issues

Accessing lithium−oxygen battery discharge products in their native environments via transmission electron microscopy grid electrode

High-fidelity and facile ex situ transmission electron microscopy (TEM) characterization of lithium−oxygen (Li−O2) batteries is still limited by challenges in preserving the native environment of Li−O2 discharge products. The extreme reactivity and moisture sensitivity of the discharge products means that they are quickly altered during sample retrieval from cycled batteries and transfer for TEM analysis, resulting in loss of original information. We here demonstrate that by using a TEM specimen grid directly in Li−O2 batteries as both support electrode and sample collector overlaid on a standard oxygen diffusion electrode, discharge products that are formed on the grid can be kept pristine.</p