Flies with mutations in CG2904 have rough eyes, defects in IOC sorting, an increase in IOC number (A-F) SEM views of adult fly eyes of various genotypes

<p><b>Copyright information:</b></p><p>Taken from ", required for interommatidial cell sorting and cell death in the pupal retina, encodes a protein with homology to ubiquitin-specific proteases"</p><p>http://www.biomedcentral.com/1471-213X/7/82</p><p>BMC Developmental Biology 2007;7():82-82.</p><p>Published online 5 Jul 2007</p><p>PMCID:PMC1950886.</p><p></p> (G-O) Pupal retinas of various genotypes stained with anti-Dlg. (A, G) Wildtype flies have regularly spaced ommatidia and an invariant number of IOCs. Cell types indicated are bristle (B), 2°, 3°, and asterisk represent extra IOCs. (B,H) flies obtained from the Bloomington Stock center have rough eyes and a modest number of extra 2° and 3° pigment cells. (C,I) GMR-driven RNAi of CG2904 results in flies with rough eyes and a large increase in IOCs, with many stacked side-by-side in parallel rows. (D,J) Flies homozygous for a deletion in CG2904, , have rough eyes, a large increase in IOCs, with many cells stacked side-by-side in parallel rows. (E,K) GMR-dependent expression of ec-SF1 has no effect on the adult eye and does not cause any excess death of IOCs. (F,L) Expression of GMR-ec-SF1 restores normal levels of IOC death to flies. (M,N) Pupal eyes from two independent stocks of outcrossed for 5 generations. There are increased numbers of IOCs as compared with the original stock, and many extra cells are aligned side-by-side in parallel rows. (O) Pupal eyes from flies have a modest increase in IOC number and few defects in cell sorting

Echinus does not require deubiquitinating activity to promote normal IOC death

<p><b>Copyright information:</b></p><p>Taken from ", required for interommatidial cell sorting and cell death in the pupal retina, encodes a protein with homology to ubiquitin-specific proteases"</p><p>http://www.biomedcentral.com/1471-213X/7/82</p><p>BMC Developmental Biology 2007;7():82-82.</p><p>Published online 5 Jul 2007</p><p>PMCID:PMC1950886.</p><p></p> (A-D) SEMs of adult eyes of various genotypes. (E-H) Pupal retinas of various genotypes stained with anti-Dlg. (A,E) GMR-driven expression of a microRNA targeting ec-SF1 results in an echinus phenotype. (B,F) eyes. (C,G) Eyes of genotype ; GMR-ec-SF2/+. (D,H) Eyes of genotype ; GMR-ec-SF3/+. Expression of versions of Echinus that lack essential USP catalytic residues rescues the phenotype

Genetic interactions between and known or potential regulators of cell death in the eye

<p><b>Copyright information:</b></p><p>Taken from ", required for interommatidial cell sorting and cell death in the pupal retina, encodes a protein with homology to ubiquitin-specific proteases"</p><p>http://www.biomedcentral.com/1471-213X/7/82</p><p>BMC Developmental Biology 2007;7():82-82.</p><p>Published online 5 Jul 2007</p><p>PMCID:PMC1950886.</p><p></p> To the right is a schematic depicting known or suggested interactions between death regulators in the fly. The question mark separating Debcl/Buffy from Ark indicates the uncertainy as to the roles these proteins play in regulating Ark activation or activity. GMR-driven transgenes of the indicated genotype were introduced into the background, or into a wildtype background in the presence of GMR-ec-SF1. For each death regulator tested, similar phenotypes were observed in the presence of GMR-ec-SF2 (data not shown)