4,735 research outputs found
Role of PknB Kinase in Antibiotic Resistance and Virulence in Community-Acquired Methicillin-Resistant Staphylococcus aureus Strain USA300
The regulation of cellular processes by eukaryote-like serine/threonine kinases is widespread in bacteria. In the last 2 years, several studies have examined the role of serine/threonine kinases in Staphylococcus aureus on cell wall metabolism, autolysis, and virulence, mostly in S. aureus laboratory isolates in the 8325-4 lineage. In this study, we showed that the pknB gene (also called stk1) of methicillin-resistant S. aureus (MRSA) strain COL and the community-acquired MRSA (CA-MRSA) strain USA300 is involved in cell wall metabolism, with the pknB mutant exhibiting enhanced sensitivity to β-lactam antibiotics but not to other classes of antibiotics, including aminoglycosides, ciprofloxacin, bactrim, and other types of cell wall-active agents (e.g., vancomycin and bacitracin). Additionally, the pknB mutant of USA300 was found to be more resistant to Triton X-100-induced autolysis and also to lysis by lysostaphin. We also showed that pknB is a positive regulator of sigB activity, resulting in compromise in its response to heat and oxidative stresses. In association with reduced sigB activity, the expression levels of RNAII and RNAIII of agr and the downstream effector hla are upregulated while spa expression is downmodulated in the pknB mutant compared to the level in the parent. Consistent with an enhanced agr response in vitro, virulence studies of the pknB mutant of USA300 in a murine cutaneous model of infection showed that the mutant was more virulent than the parental strain. Collectively, our results have linked the pknB gene in CA-MRSA to antibiotic resistance, sigB activity, and virulence and have highlighted important differences in pknB phenotypes (virulence and sigB activity) between laboratory isolates and the prototypic CA-MRSA strain USA300
On a problem of A. Weil
A topological invariant of the geodesic laminations on a modular surface is
constructed. The invariant has a continuous part (the tail of a continued
fraction) and a combinatorial part (the singularity data). It is shown, that
the invariant is complete, i.e. the geodesic lamination can be recovered from
the invariant. The continuous part of the invariant has geometric meaning of a
slope of lamination on the surface.Comment: to appear Beitr\"age zur Algebra und Geometri
Assessing NARCCAP climate model effects using spatial confidence regions
We assess similarities and differences between model effects for the North American Regional
Climate Change Assessment Program (NARCCAP) climate models using
varying classes of linear regression models. Specifically, we
consider how the average temperature effect differs for the various
global and regional climate model combinations, including assessment
of possible interaction between the effects of global and regional
climate models. We use both pointwise and simultaneous inference
procedures to identify regions where global and regional climate model effects differ. We also show
conclusively that results from pointwise inference are misleading, and
that accounting for multiple comparisons is important for making
proper inference
Response of VIRGO detectors to pre-big-bang gravitons
The sensitivity achievable by a pair of VIRGO detectors to stochastic and
isotropic gravitational wave backgrounds produced in pre-big-bang models is
discussed in view of the development of a second VIRGO interferometer. We
describe a semi-analytical technique allowing to compute the signal-to-noise
ratio for (monotonic or non-monotonic) logarithmic energy spectra of relic
gravitons of arbitrary slope. We apply our results to the case of two
correlated and coaligned VIRGO detectors and we compute their achievable
sensitivities. We perform our calculations both for the usual case of minimal
string cosmological scenario and in the case of a non-minimal scenario where a
long dilaton dominated phase is present prior to the onset of the ordinary
radiation dominated phase. In this framework, we investigate possible
improvements of the achievable sensitivities by selective reduction of the
thermal contributions (pendulum and pendulum's internal modes) to the noise
power spectra of the detectors. Since a reduction of the shot noise does not
increase significantly the expected sensitivity of a VIRGO pair (in spite of
the relative spatial location of the two detectors) our findings support the
experimental efforts directed towards a substantial reduction of thermal noise.Comment: 23 pages in Latex styl
Ergodic Jacobi matrices and conformal maps
We study structural properties of the Lyapunov exponent and the
density of states for ergodic (or just invariant) Jacobi matrices in a
general framework. In this analysis, a central role is played by the function
as a conformal map between certain domains. This idea goes
back to Marchenko and Ostrovskii, who used this device in their analysis of the
periodic problem
Insecurity for compact surfaces of positive genus
A pair of points in a riemannian manifold is secure if the geodesics
between the points can be blocked by a finite number of point obstacles;
otherwise the pair of points is insecure. A manifold is secure if all pairs of
points in are secure. A manifold is insecure if there exists an insecure
point pair, and totally insecure if all point pairs are insecure.
Compact, flat manifolds are secure. A standing conjecture says that these are
the only secure, compact riemannian manifolds. We prove this for surfaces of
genus greater than zero. We also prove that a closed surface of genus greater
than one with any riemannian metric and a closed surface of genus one with
generic metric are totally insecure.Comment: 37 pages, 11 figure
Description of the Scenario Machine
We present here an updated description of the "Scenario Machine" code. This
tool is used to carry out a population synthesis of binary stars. Previous
version of the description can be found at
http://xray.sai.msu.ru/~mystery//articles/review/contents.htmlComment: 32 pages, 3 figures. Corrected typo
Jet Substructure at the Tevatron and LHC: New results, new tools, new benchmarks
In this report we review recent theoretical progress and the latest
experimental results in jet substructure from the Tevatron and the LHC. We
review the status of and outlook for calculation and simulation tools for
studying jet substructure. Following up on the report of the Boost 2010
workshop, we present a new set of benchmark comparisons of substructure
techniques, focusing on the set of variables and grooming methods that are
collectively known as "top taggers". To facilitate further exploration, we have
attempted to collect, harmonise, and publish software implementations of these
techniques.Comment: 53 pages, 17 figures. L. Asquith, S. Rappoccio, C. K. Vermilion,
editors; v2: minor edits from journal revision
Weak Liouville-Arnold Theorems & Their Implications
This paper studies the existence of invariant smooth Lagrangian graphs for
Tonelli Hamiltonian systems with symmetries. In particular, we consider Tonelli
Hamiltonians with n independent but not necessarily involutive constants of
motion and obtain two theorems reminiscent of the Liouville-Arnold theorem.
Moreover, we also obtain results on the structure of the configuration spaces
of such systems that are reminiscent of results on the configuration space of
completely integrable Tonelli Hamiltonians.Comment: 24 pages, 1 figure; v2 corrects typo in online abstract; v3 includes
new title (was: A Weak Liouville-Arnold Theorem), re-arrangement of
introduction, re-numbering of main theorems; v4 updates the authors' email
and physical addresses, clarifies notation in section 4. Final versio
Frictional drag between quantum wells mediated by phonon exchange
We use the Kubo formalism to evaluate the contribution of acoustic phonon
exchange to the frictional drag between nearby two-dimensional electron
systems. In the case of free phonons, we find a divergent drag rate
(). However, becomes finite when phonon
scattering from either lattice imperfections or electronic excitations is
accounted for. In the case of GaAs quantum wells, we find that for a phonon
mean free path smaller than a critical value, imperfection
scattering dominates and the drag rate varies as over many
orders of magnitude of the layer separation . When exceeds the
critical value, the drag rate is dominated by coupling through an
electron-phonon collective mode localized in the vicinity of the electron
layers. We argue that the coupled electron-phonon mode may be observable for
realistic parameters. Our theory is in good agreement with experimental results
for the temperature, density, and -dependence of the drag rate.Comment: 45 pages, LaTeX, 8 postscript file figure
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